Primary cells of human rectal mucosal epithelium

All products of our company are for scientific experiments only and are not intended for use outside of scientific experiments!

Product Name:Primary cells of human rectal mucosal epithelium
English name:Human Rectal Mucosal Epithelial Cells
Specifications5×10⁵Cells/T25culture flask
Item No.GOY-01X0844
Classification:Human primary cells

Growth characteristics: Wall adhesion

Cell morphology:Epithelial like cells

Isolation of human rectal mucosal epithelial cells from rectal tissue; The rectum is the terminal segment of the large intestine, located within the pelvis. Upper end flat3The coccyx is connected to the sigmoid colon, descending along the front of the sacrum and coccyx, passing through the pelvic diaphragm, and ending at the lower end. The upper end of the rectum is similar in size to the colon, and its lower end expands into the ampulla of the rectum, which is a temporary storage site for feces before excretion. The lower end becomes thinner. The position of the rectum in the pelvic cavity is closely related to the ventral surface of the sacrum and has the same curvature as the sacrum. The rectum is surrounded by fat and lacks longitudinal bands, located on the dorsal side of the bladder and reproductive organs. The arterial blood supply to the rectum mainly comes from the superior rectal artery of the inferior mesenteric artery, the middle rectal artery of the internal iliac artery, and the inferior rectal artery of the internal iliac artery. Intestinal mucosal epithelial cells are an important barrier for the internal and external environment of the body, continuously exposed to a large amount of antigens, and also the first line of defense for the body against pathogenic microorganisms. Therefore, in addition to important physiological functions such as absorption, secretion, and transport, intestinal mucosal epithelial cells also play an important role in the innate and acquired immune defense mechanisms of the mucosa. As the first cells to come into contact with antigens, intestinal mucosal epithelial cells play a crucial role in the initial stage of mucosal immune response, determining the occurrence, nature, and intensity of mucosal immune response.

Method Introduction:

The human rectal mucosal epithelial cells isolated in the company's laboratory were prepared by mechanical separation followed by collagenase digestion and selected through epithelial cell specific culture medium. The total number of cells was approximately5×10?cells/Bottle.

Quality inspection:

The human rectal mucosal epithelial cells isolated from the company laboratoryCytokeratin-18Immunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.

1. Preparation of culture medium and culture cryopreservation conditions:

Prepare DMEM high sugar, 10% high-quality fetal bovine serum.

1) Cultivation conditions: Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%.

2) Cryogenic solution: 90% culture medium, 10% DMSO, ready to use and prepared. Liquid nitrogen storage.

2. Cell processing:

1) Resuscitate cells: Quickly shake and thaw a cryovial containing 1mL of cell suspension in a 37 ℃ water bath, then add 4mL of culture medium and mix well. Centrifuge at 1000RPM for 4 minutes, discard the supernatant, add 1-2mL of culture medium, and blow evenly. Then add all cell suspensions to the culture bottle and culture overnight (or add the cell suspensions to a 10cm dish, add about 8ml of culture medium, and culture overnight). The next day, change the solution and check the cell density.

2) Cell passage: If the cell density reaches 80% -90%, passage culture can be carried out.

3) Cell cryopreservation: When the cells are in good growth condition, cell cryopreservation can be performed. When freezing adherent cells, discard the culture medium and add a small amount. After the cells become round and fall off, add about 1ml of serum containing culture medium to the freezing tube, and then add 10% DMSO for freezing.

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1. After receiving the cells, first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please contact us in a timely manner.

2. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.

3. Wipe the surface of the cell vial with 75% alcohol and observe the cell state under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall. The cells should be placed in a culture box and incubated overnight before being taken out for observation the next day. At this point, most cells will adhere to the wall. If the cells still cannot adhere to the wall, please use trypan blue staining to determine cell viability. If it is confirmed that the cell viability is normal, please centrifuge the cells and culture them again with fresh culture medium; If the staining result shows that the cells are inactive, please take a photo and contact us in a timely manner. After confirming the information, we will send it to you again for free.

4. Please ask the customer to use the same culture medium for cell culture under the same conditions. Excess culture medium in the culture bottle can be collected for future use. When cells are passaged, they can be mixed in a certain proportion with the culture medium provided by the customer to gradually adapt to the culture conditions; Suggest purchasing the provided culture medium directly.

5. It is recommended that customers take several photos of the cells in the first 3 days after receiving them, recording the cell status for communication and exchange with the technical department of the Microbial Strain Query Network.

6. This cell can only be used for scientific research and cannot be used for clinical applications.