Primary cells of human myocardium

All products of our company are for scientific experiments only and are not intended for use outside of scientific experiments!

Product Name:Primary cells of human myocardium
English name:Human Cardiomyocyte Cells
Specifications5×10⁵Cells/T25culture flask
Item No.GOY-01X0955
Classification:Human primary cells

Growth characteristics: Wall adhesion

Cell morphology:Shuttle shaped, polygonal shaped

Isolation of human cardiac myocytes from cardiac tissue; The heart is an important organ in the vertebrate body, primarily responsible for providing pressure for blood flow and transporting blood to various parts of the body. The heart is composed of myocardial tissue, consisting of four chambers: the left atrium, left ventricle, right atrium, and right ventricle. The left and right atria and ventricles are separated by a septum, so they are not connected to each other. There are valves (atrioventricular valves) between the atria and ventricles, which allow blood to flow only from the atria into the ventricles and not backwards. The function of the heart is to promote blood flow, provide sufficient blood flow to organs and tissues, with oxygen and various nutrients, and carry away metabolic end products (such as carbon dioxide, inorganic salts, urea, and uric acid), allowing cells to maintain normal metabolism and function. Cardiomyocytes have irregular shapes such as diamonds and polygons; cell culture2hThen start to stick to the wall, forming a shuttle shape; to12hOn both sides, cells begin to extend pseudopodia, forming diamond shaped and polygonal shapes; Cell isolation and culture48hIn the future, most of the pseudopodia will extend and form a palmar shape, and some myocardial cells will experience pulsation; Cardiomyocytes are terminally differentiated cells and do not proliferate in vitro. In vitro cultured cardiomyocytes can maintain certain structural and functional characteristics, and have spontaneous rhythmic beats. The cultivation of cardiomyocytes is characterized by simplicity, quantification, good reproducibility, and is not affected by neurohumoral factors. The use of cultured myocardial cells to explore the regulation of myocardial hypertrophy caused by non hemodynamic factors, study the biomechanics, apoptosis, receptor downregulation, ischemic preconditioning, signaling pathways of myocardial cells, screen and evaluate the properties of new drugs acting on the heart, and extract valuable biological factors from cultured myocardial cells have broad application prospects. It is of great significance for studying their physiological functions, drug effects, and pathological and physiological changes under various pathogenic factors.

Method Introduction:

The human cardiomyocytes isolated in the company laboratory were treated with collagenase-It is prepared by combining the combined digestion method with differential adhesion method and screening with chemical reagent inhibition method, with a total cell count of approximately5×10?cells/Bottle.

Quality inspection:

The human cardiomyocytes isolated from the company's laboratory were subjected to alpha irradiation-Sarcometric actinImmunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.

1. Preparation of culture medium and culture cryopreservation conditions:

Prepare DMEM high sugar, 10% high-quality fetal bovine serum.

1) Cultivation conditions: Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 degrees Celsius, humidity in the incubator is 70% -80%.

2) Cryogenic solution: 90% culture medium, 10% DMSO, ready to use and prepared. Liquid nitrogen storage.

2. Cell processing:

1) Resuscitate cells: Quickly shake and thaw a cryovial containing 1mL of cell suspension in a 37 ℃ water bath, then add 4mL of culture medium and mix well. Centrifuge at 1000RPM for 4 minutes, discard the supernatant, add 1-2mL of culture medium, and blow evenly. Then add all cell suspensions to the culture bottle and culture overnight (or add the cell suspensions to a 10cm dish, add about 8ml of culture medium, and culture overnight). The next day, change the solution and check the cell density.

2) Cell passage: If the cell density reaches 80% -90%, passage culture can be carried out.

3) Cell cryopreservation: When the cells are in good growth condition, cell cryopreservation can be performed. When freezing adherent cells, discard the culture medium and add a small amount. After the cells become round and fall off, add about 1ml of serum containing culture medium to the freezing tube, and then add 10% DMSO for freezing.

1. After receiving the cells, first observe whether the cell bottle is intact and whether there is any leakage or turbidity in the culture medium. If any of the above phenomena occur, please contact us in a timely manner.

2. Carefully read the cell manual to understand cell related information, such as cell morphology, culture medium used, serum ratio, required cytokines, etc.

3. Wipe the surface of the cell vial with 75% alcohol and observe the cell state under a microscope. Due to transportation issues, a small amount of adherent cells may detach from the bottle wall. The cells should be placed in a culture box and incubated overnight before being taken out for observation the next day. At this point, most cells will adhere to the wall. If the cells still cannot adhere to the wall, please use trypan blue staining to determine cell viability. If it is confirmed that the cell viability is normal, please centrifuge the cells and culture them again with fresh culture medium; If the staining result shows that the cells are inactive, please take a photo and contact us in a timely manner. After confirming the information, we will send it to you again for free.

4. Please ask the customer to use the same culture medium for cell culture under the same conditions. Excess culture medium in the culture bottle can be collected for future use. When cells are passaged, they can be mixed in a certain proportion with the culture medium provided by the customer to gradually adapt to the culture conditions; Suggest purchasing the provided culture medium directly.

5. It is recommended that customers take several photos of the cells in the first 3 days after receiving them, recording the cell status for communication and exchange with the technical department of the Microbial Strain Query Network.

6. This cell can only be used for scientific research and cannot be used for clinical applications.

CM-R032Rat intestinal vein endothelial cellsC3H/10T1/2, Clone 8(Mouse embryonic fibroblasts) LRRN3 Others HumanpersonLRRN3Rod-shaped virus-Insect cell lysate OPTN Others HumanpersonOptineurin / OPTNRod-shaped virus-Insect cell lysis of mouse primary glomerular mesangial cells and mouse primary ovarian endometrial cells

TSFP1/SP1(transcription factor Sp1 0.5mgTSFP1/SP1(transcription factor Sp1) SP1Transcription growth factor antigen

ITIH3 Protein HumanRecombinant humanITIH3protein(Fclabel)

G-250Protein imprinting membrane rapid staining reagent2*100ml

IL12B Protein HumanRecombinant humanIL12B / P40protein

MAPK8Recombinant humanJNK1 / MAPK8protein(GSTlabel) Protein

Human tumor suppressorM(OSM)ELISAtest kit

Human ansferrin receptor (TFR/CD71) ELISA KitHuman transferrin receptor(TFR/CD71)test kit

Humahymidinephosphorylase,TPELISAKitHuman nucleoside0PNPase(TP)test kit96T/48TImport packaging

HumanIerleukin16,IL-16Reagent kit for human interleukin16(IL-16)Kit specifications:96T/48T

Organic soluble total protein preparation kit20time

Humane lefoilfactor, itfelisakitHuman intestinal trefoil factor(ITF)Kit specifications:96T/48T

Human apolipoproteinEtest kit Human apolipoproteinEtest kit Specification Model96T/48T Human apolipoproteinEReagent kit, specification and model:96T/48TSource: reagent kit (imported packaging), product alias: human apolipoproteinEReagent kit, human apolipoproteinEtest kit Storage conditions:2-8℃ quality guarantee period:6A month.

Human apolipoproteinC3test kit Human apolipoproteinC3test kit Specification Model96T/48T Human apolipoproteinC3Reagent kit, specification and model:96T/48TSource: reagent kit (imported packaging), product alias: human apolipoproteinC3Reagent kit, human apolipoproteinC3test kit Storage conditions:2-8℃ quality guarantee period:6A month.

Human apolipoproteinB100test kit Human apolipoproteinB100test kit Specification Model96T/48T Human apolipoproteinB100Reagent kit, specification and model:96T/48TSource: reagent kit (imported packaging), product alias: human apolipoproteinB100Reagent kit, human apolipoproteinB100test kit Storage conditions:2-8℃ quality guarantee period:6A month.

Human apolipoproteinA5test kit Human apolipoproteinA5test kit Specification Model96T/48T Human apolipoproteinA5Reagent kit, specification and model:96T/48TSource: reagent kit (imported packaging), product alias: human apolipoproteinA5Reagent kit, human apolipoproteinA5test kit Storage conditions:2-8℃ quality guarantee period:6A month.

Primary cells of human myocardiumGuinea pig interleukin10(IL-10)Reagent kit, English name:IL-10 ELISA Kit

Human non neuronal enolase (NNE) ELISA KitHuman non neuronal enolase(NNE)test kit

rabbitiestinalfattyacidbindingprotein,iFABPELISAKitRabbit intestinal fatty acid binding protein(iFABP)test kit96T/48TImport packaging

Cliakiskan 2 -gp 1 IgA / g / meliillnessRat β2glycoprotein1antibodyIgA/G/M

Bacterial nutrient agar plate50a

RabbitEpidermalgrowthfactor,EGFELISAKitRabbit epidermal growth factor(EGF)Kit specifications:96T/48T

Crystal violet staining solution100ml

TrK A (h-NGFR 0.5mgTrK A (h-NGFR) (p140- TrK A)A type of nerve growth factor(antigen)

CD226Recombinant humanCD226 / DNAM-1proteinProtein

GYPC Protein HumanRecombinant humanGYPC / Glycophorin-Cprotein

NBL1 Protein HumanRecombinant humanNBL1 / DAND1 / DANprotein