Precautions for operating the bovine growth hormone (GH) ELISA kit

Preparation of experimental materials and reagents:

1. Instruments and materials: ELISA reader (preheated for 30 minutes before use), micropipette, pipette, distilled water or deionized water, filter paper.

2. Dilution of 20 x washing buffer: Dilute distilled water at a ratio of 1:20, which means adding 19 parts of distilled water to 1 part of 20 x washing buffer.

Sample collection, processing, and preservation:Precautions for operating the bovine growth hormone (GH) ELISA kit

Cell culture supernatant: suitable for detecting secreted components of cells cultured in vitro. Collect the cell supernatant using a sterile tube, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

Serum: At room temperature, blood naturally coagulates, centrifuge at 1000 × g for 15 minutes, and collect the supernatant for testing.

【 Plasma 】: EDTA or sodium citrate should be selected as anticoagulants according to the requirements of the specimen. After mixing, let it stand for 10-20 minutes, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

【 Body fluids 】: including pleural and peritoneal fluid, cerebrospinal fluid, secretions, etc. Collect using a centrifuge tube without pyrogen and endotoxin, centrifuge at 1000 × g for 15 minutes, and collect the supernatant.

: The sample cannot contain NaN3, as NaN3 inhibits the activity of horseradish peroxidase (HRP).

: If the sample cannot be immediately detected, it should be packaged and stored at -80 ℃ to avoid repeated freezing. If precipitation occurs during storage, it should be centrifuged again. Do not use hemolytic or hyperlipidemic blood samples as much as possible. If there are a large number of particles in the serum, centrifuge or filter it before testing. Do not thaw by heating at 37 ℃ or higher. Thaw at room temperature and ensure that the sample is evenly thawed.

Precautions for operating the bovine growth hormone (GH) ELISA kit：

1) Strictly follow the prescribed time and temperature for incubation to ensure accurate results. All reagents must reach room temperature of 20-25 ℃ before use and be immediately refrigerated after use.

2) Incorrect washing of the plate can lead to inaccurate results. Before adding the substrate, make sure to absorb as much liquid as possible from the well, and do not let the micropores dry during the incubation process.

3) Eliminate residual liquid and fingerprints on the bottom plate, otherwise it will affect the OD value.

4) The substrate color solution should be colorless or very light in color, and the substrate solution that has turned blue cannot be used.

5) Avoid cross contamination of test kit specimens to prevent erroneous results.

6) Any reaction reagents should not come into contact with bleach solvents or the strong gases emitted by bleach solvents to avoid affecting the biological activity of the reagents.

7) After balancing to room temperature, open the sealing bag to prevent water droplets from condensing on the cold Flat noodles.

Our commonly used testing indicators are:

1. Cytokine detection (such as interleukin, necrosis factor, interferon, transforming growth factor, chemokine, cytokine receptor, colony-stimulating factor, adhesion molecule, growth factor, etc.)

2. Endocrine testing (such as thyroid, pancreas, sex, progesterone, growth, endothelin, osteocalcin, prolactin, testosterone, adrenocorticotropic hormone, follicle stimulating hormone, estradiol, estriol, serotonin, 17 hydroxyprogesterone, etc.)

3. Myocardial infarction testing (such as troponin, myoglobin, C-reactive protein, etc.)

4. Liver fibrosis detection (such as fibronectin, hyaluronic acid, collagen, matrix metalloproteinase factor, matrix metalloproteinase, laminin, etc.)