Plasma mutagenesis breeding instrument

NegotiableUpdate on 01/04

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Overview

Plasma mutagenesis breeding instrument with atmospheric pressure and room temperature plasma is a new type of microbial mutagenesis breeding method. High purity helium gas is discharged in a high-frequency electric field to generate plasma, which is rich in high-energy chemically active particles that can have multiple effects on bacterial strains/plant cells.

Product Details

常压室温等离子体诱变育种仪

1、 Product Introduction

Plasma mutagenesis breeding instrumentCompared with the traditional low-pressure gas discharge plasma source, atmospheric room temperature plasma (ARTP) has the characteristics of low plasma jet temperature, uniform discharge and high concentration of chemically active particles. Based on ARTP technology, our company, together with relevant teams of Tsinghua University, has developed a special instrument for mutation breeding of microorganisms by means of plasma - ARTP Mutagenesis Breeding Machine. The instrument has a high mutation rate, compact structure, easy operation, high safety, and fast mutation speed. A large capacity mutation library can be obtained in one mutation operation (within a few minutes), greatly improving the intensity and capacity of strain mutation; ARTP technology combined with high-throughput screening technology can achieve rapid and efficient evolutionary breeding of organisms.

Plasma mutagenesis breeding instrumentNon GMO methods ensure the safety of organisms

Wide range of applications, high mutation performance

Exclusive helium plasma mutagenesis technology, high energy, strong gene damage intensity

Easy to operate, safe, easy to maintain, and low operating costs

2、 Application areas:
Prokaryotes (such as bacteria, actinomycetes, etc.), eukaryotes (such as molds, yeasts, algae, higher fungi, etc.), and plant cells.
As of October 21, 2022, there are 411 Chinese literature and 169 English literature, patents269 papers, 176 dissertations, totaling 1025 papers
3、 Technical parameters

classification	Technical Specifications
Total machine power	500W（MAX）
Discharge technology	Atmospheric pressure uniform glow discharge, plasma jet uniform and stable
Working gas	99.999% or higher high-purity helium gas
Gas volume control range	0~15SLM (standard liters/minute)
Gas volume control accuracy	± 1.0% F.S. (full scale)
Effective handling of spacing	2 mm
Sample processing system	Continuous processing and automatic collection of 6 samples
cooling system	External refrigeration system
Plasma jet temperature	≤37℃
Work environment requirements	Temperature 15~25 ℃, humidity ≤ 60% (recommended to be placed in a clean room)
Scope of application	Prokaryotes (such as bacteria, actinomycetes, etc.), eukaryotes (such as mold, yeast, algae, higher fungi, etc.)

4、 Application case:
Case 1: Application of ARTP mutagenesis to induce high production of pyrroloquinoline quinone in Pseudomonas aeruginosa AM1

常压室温等离子体诱变育种仪

Figure 4 Batch Fermentation Results of M. extorquens AM1 Wild Strain (A) and Positive Mutant E-F3 (B) Fig. 4 Batch fermentation of M. extorquens AM1 (A) and E-F3 (B).

Using atmospheric pressure room temperature plasma (ARTP) for mutagenesis, combined with high-throughput rapid screening methods, a forward mutant strain with PQQ yield as the indicator was obtained. The strain induced by ARTP had a positive mutation rate of 31.6%, and the selected superior positive mutant strain M. extorquens AM1 (E-F3) achieved a yield of 54.0 mg/L, nearly three times that of the starting strain. (Journal of Bioengineering, 2016, 32 (8): 1145-1149)
Case 2: Application of ARTP to enhance the enzymatic activity of glutaminase produced by Streptococcus mutans

常压室温等离子体诱变育种仪

Typical colony characteristics after ARTP mutagenesis (G1-G14 represents typical colonies with different morphology from the starting strain); G15 has the same morphology as the starting strain)

The ARTP technique was used to induce mutations in Streptomyces spores, with a mutation rate of 42.8% and a positive mutation rate of 20.6%. The G2-1 enzyme activity of the high-yield mutant strain reached 2.73U/mL, which was higher than that of the starting strainimprove82%. (Microbiology Bulletin, 2010, 37 (11): 1642-1649)