Mouse nucleus pulposus cells

Cell Description
The nucleus pulposus is a milky white translucent gelatinous body, rich in elasticity, and is part of the intervertebral disc structure, located between the two cartilage plates and the annulus fibrosus. Elastic jelly material composed of criss crossing fibrous network structures, namely chondrocytes and proteoglycan mucinous matrix. The premature aging and apoptosis of nucleus pulposus cells are one of the main causes of intervertebral disc degeneration, mainly manifested as a decrease in the function and quantity of nucleus pulposus cells in degenerated intervertebral discs, resulting in a decrease in the secretion of matrix such as type II collagen polysaccharides, ultimately leading to the loss of biomechanical functions such as maintaining spinal height and bearing various stresses in intervertebral discs.
Cell characteristics
1) The tissue is derived from normal intervertebral disc tissue of experimental animals.
2) Cell identification: Collagen II immunofluorescence staining was positive.
3) After identification, the purity of the cells was found to be higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
5) Cell growth mode: spindle shaped or polygonal cells, irregular cells, adherent culture.
Transportation and storage of products
Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.
1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.
2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.
Product Usage
1) This product can only be used for scientific research purposes
2) This productNot approved for direct use in live animals and humans
3) This product has not passed the review for in vivo diagnosis