Mouse annulus fibrosus cells

Cell Description
The annulus fibrosus, located at the periphery of the intervertebral disc, is composed of fibrocartilage. The fibers of the annulus fibrosus run obliquely between the vertebral bodies and are arranged in concentric rings in cross-section. The fibers of adjacent rings have opposite slopes and intersect with each other. Fiber ring cells are located in front of collagen fiber bundles, synthesizing and secreting extracellular matrix of the fiber ring to maintain normal fiber ring structure and function.
Cell characteristics
1) The tissue is derived from normal intervertebral disc tissue of experimental animals.
2) Cell identification: Immunofluorescence staining for type II collagen or type I collagen is positive.
3) After identification, the purity of the cells was found to be higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
5) Cell growth mode: polygonal cells, irregular cells, adherent culture.
Transportation and storage of products
Depending on the weather conditions and transportation distance, the company and the customer will negotiate and choose one of the following methods.
1) 1mL cryopreserved cell suspension is put into 1.8ml cryopreserved tube and transported in foam insulation box filled with dry ice; After receiving the cells, please thaw and revive them for cultivation as soon as possible. If resuscitation cannot be performed immediately, the frozen cells can be stored at -80 ℃ for one month.
2) The T-25 culture bottle is filled with culture medium and transported at room temperature; After receiving the cells, please observe the growth status of the cells under a microscope. If the bottle laying rate exceeds 85%, immediately perform passaging operation. If there are many suspended cells, please place the culture bottle in the incubator overnight to help non dead suspended cells adhere to the wall again.
Product Usage
1) This product can only be used for scientific research purposes
2) This productNot approved for direct use in live animals and humans
3) This product has not passed the review for in vivo diagnosis