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Building C, No. 888 Huanhu West Second Road, Lingang New Area, China (Shanghai) Pilot Free Trade Zone
Changdaen Biotechnology (Shanghai) Co., Ltd
Building C, No. 888 Huanhu West Second Road, Lingang New Area, China (Shanghai) Pilot Free Trade Zone
Product advantages of Changdaen Biotechnology (Shanghai) Co., Ltd.:
1. Quality assurance: Our company's products have the characteristics of high sensitivity, strong specificity, and good repeatability. The variety is complete, the operation is simple, and the experimental expenses are saved to a limited extent;
2. Reliable Quality: Strict quality control ensures that 100% of the products provided to customers are of high quality, with full cold chain transportation. The company promises to provide returns and exchanges for any quality issues (not caused by human factors), operate with integrity, and cooperate for win-win results;
3. Free testing: The company promises to order Changdaen Biological ELISA kits and enjoy full technical guidance and free testing services;
4. Worry free after-sales service: 7 * 24-hour after-sales service, truly achieving worry free after-sales service and continuous service system;
5. Professional customization: ELISA kits, biochemical kits, etc. that meet customer needs such as species, detection range, sensitivity, etc. can be customized according to customer requirements
Choose our 4 main reasons:
1. First level agent in mainland China, legitimate and legitimate procurement channels, with numerous agent brands;
2. Full cold chain transportation ensures product quality;
3. Pre sales and after-sales services, providing product consultation;
4. A professional technical support team ensures the correct use of the product.
Mouse ceramide-1-phosphate (C1P) ELISA kit
Featured Services: Provide free testing services and one-on-one technical guidance.
Item number: EN-XS94547
Detection index: Ceramide-1-phosphate
Specification: Consult customer service
Samples: serum, plasma, cell supernatant, urine, body fluids, lavage fluid, cerebrospinal fluid, atrial fluid, pleural fluid, tissue, etc.
Sample collection, processing, and preservation methods
1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.
2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.
3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.
4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.
Personal belongings
1. ELISA reader (450nm)
2. High precision sampler and nozzle: 0.5-10uL, 2-20uL, 20-200uL, 200-1000uL
3. 37 ℃ constant temperature box
Operation precautions
1. The reagent kit should be stored at 2-8 ℃ and equilibrated at room temperature for 20 minutes before use. The concentrated washing solution taken out of the refrigerator will have crystals, which is a normal phenomenon. Heating in a water bath will dissolve the crystals before use.
2. The Flat noodles not used in the experiment shall be immediately put back into the self sealing bag and sealed (low-temperature dry) for storage.
The S0 standard with a concentration of 0 can be considered as a negative control or blank; When operating according to the instructions, the sample has already been diluted 5 times, and the final result multiplied by 5 is the actual concentration of the sample.
4. Strictly follow the time, liquid dosage, and sequence indicated in the instructions for incubation operation.
5. Shake all liquid components thoroughly before use.
Mouse ceramide-1-phosphate (C1P) ELISA kit
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Mouse ceramide-1-phosphate (C1P) ELISA kit