Mouse bile salt hydrolase (BSH) ELISA kit

Mouse bile salt hydrolase (BSH) ELISA kitDetection Principle

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay（ELISA）。 Add the specimenStandard and HRP labeled detection antibodies were incubated and washed. Using substrate TMB for color development, TMB is converted to blue under the catalysis of peroxidase and to the final yellow under the action of acid. The depth of color is positively correlated with the indicators in the sample. Measure the absorbance (OD value) using an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 450nm and calculate the sampleConcentration.

Mouse bile salt hydrolase (BSH) ELISA kitSample collection, processing, and preservation methods

1. Serum: Use test tubes free of pyrogens and endotoxins, avoid any cell irritation during the operation, collect blood, centrifuge at 3000 rpm for 10 minutes, and quickly and carefully separate serum and red blood cells.

2. Plasma: anticoagulant with EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant.

3. Cell supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers.

4. Tissue homogenate: Crush the tissue by adding an appropriate amount of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.

5. Storage: If the sample is not tested in a timely manner after collection, please divide it into batches according to a single dose, freeze it at -20 ℃, avoid repeated freezing and thawing, thaw it at room temperature, and ensure that the sample is evenly filled and thawed.

Personal belongings

1. ELISA reader（450nm）

2. High precision sampler and gun head:0.5-10uL、2-20uL、20-200uL、200-1000uL

3. 37 ℃ constant temperature box

Product advantages of Changdaen Biotechnology (Shanghai) Co., Ltd.:

1.Quality assurance: Our products have the characteristics of high sensitivity, strong specificity, and good repeatability, with a complete variety, easy operation, and limited savings in experimental costs;

2.Reliable quality: strict quality control to ensure the quality provided to customers100%It is a high-quality product with full cold chain transportation. The company promises to provide returns and exchanges for any quality issues (not caused by human factors), operate with integrity, and cooperate for win-win results;

3.Free testing: The company promises to order Changda'en BiotechnologyELISAReagent kit, enjoy full technical guidance and free testing services;

4.Worry free after-sales service:7*24Hourly after-sales service, truly achieving worry free after-sales service and continuous service system;

5.Professional customization: Species, detection range, sensitivity, etc. can be customized according to customer needs to meet customer requirementsELISAtest kit

Choose us4Reasons for Dali:

1First level agent in mainland China, legitimate and legitimate procurement channels, with numerous agent brands;

2Full process cold chain transportation to ensure product quality;

3Pre sales and after-sales services, providing product consultation;

4A professional technical support team ensures the correct use of the product.

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