Mouse cGAMP ELISA detection kit

Mouse cGAMP ELISA detection kit

Mouse cyclic guanosine monophosphate adenosine monophosphate (cGAMP) ELISA detection kit

Product Overview

This test kit is based onDouble antibody sandwich method The high-sensitivity enzyme-linked immunosorbent assay (ELISA) tool is designed specifically for quantitative analysis of cyclic GMP AMP (cGAMP) in mouse serum, plasma, tissue homogenates, cell culture supernatants, and other samples. CGAMP, as a key second messenger in the STING signaling pathway, plays an important role in natural immune regulation, antiviral response, and tumor immune microenvironment. This kit uses high affinity anti mouse cGAMP monoclonal antibodies, combined with an optimized detection process, to achieve precise quantification of trace amounts of cGAMP.

Detection principle (sandwich method)

1. Capture antibody pre packagingPre coated with capture antibodies targeting cGAMP specific epitopes in the micropores of the enzyme-linked immunosorbent assay (ELISA) plate.

2. Antigen bindingCGAMP in the sample binds to capture antibodies to form an "antibody antigen" complex.

3. Detecting antibody bindingAdd cGAMP to detect antibodies, bind to another epitope of the antigen, and form a "sandwich" structure.

4. Signal amplification and detectionStreptomycin HRP catalyzes the color development of TMB substrate, and the absorbance value is positively correlated with the concentration of cGAMP in the sample.

5. quantitative analysisCalculate the specific concentration of cGAMP in the sample using a standard curve.

core advantage

1. Ultra high sensitivityDetection limit as low as0.01 pg/mLThe sandwich method has significantly better sensitivity than the competitive method and is suitable for ultra-low abundance samples.

2. high specificityDouble antibodies target different epitopes of cGAMP to avoid cross reactivity (such as interference from cAMP, GMP, etc.).

3. Wide linear rangeStandard curve coverage meets the requirements for samples ranging from low to medium to high concentrations.

4. Multi scenario compatibilitySupports complex sample types such as serum, plasma, cerebrospinal fluid, urine, and cell lysate.

5. efficient operationPre packaged board design+stable color rendering system, the entire process only requires1.5 hoursCompetition law usually takes longer.

6. Reliable resultsThrough international standard calibration, the difference between batches is less than 8%, which complies with GLP/cGMP regulations.

Application scenarios

• Immunological researchMonitoring the activation status of STING pathway in viral infections and tumor models;

• drug developmentEvaluate the regulatory effect of STING agonists/inhibitors on cGAMP metabolism;

• Disease mechanismAnalyze the dynamic changes of cGAMP in neurodegenerative diseases and autoimmune diseases;

Usage process

1. sample processingTo avoid hemolysis in serum/plasma, homogenize the tissue sample and centrifuge to obtain the supernatant.

2. Add sample incubationGradient dilution standard and test sample are added to pre coated plates for incubation.

3. signal detectionAdd the streptavidin HRP TMB colorimetric stop buffer to the reaction.

4. data analysisRead the OD value at a wavelength of 450 nm and calculate the concentration by fitting the standard curve with software.

5. Quality control suggestionsEach board is equipped with blank holes, standard curve holes, and internal reference points to ensure data accuracy.

Storage and precautions

• Storage conditionsUnopened reagent kit stored at 2-8 ° C (away from light), valid for 12 months; After opening, the strips shall be sealed and kept dry.

• Precautions：

• Wear gloves during operation to avoid contact with skin or mucous membranes;

• If the sample is turbid or has sediment, centrifuge at 10000 × g for 10 minutes and then take the supernatant;

• Avoid repeated freezing and thawing of standard products, it is recommended to pack and store them separately.

Order Information

provide96 servings per box Standard specifications, including all reagents and consumables (enzyme-linked immunosorbent assay plates, standards, antibodies, HRP labeled streptavidin, TMB substrates, stop solutions, etc.), supporting customized services.

throughSandwich ELISA Technology, this kit can accurately capture the dynamic changes of trace amounts of cGAMP in samples, providing reliable data support for the study of immune regulation mechanisms and disease diagnosis and treatment! If you have further questions, please feel free to contact the company's sales at any time.

Mouse cGAMP ELISA detection kit