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Hangzhou Xunshu Technology Co., Ltd
Micronucleus analysis/colony counting analyzer
NegotiableUpdate on 05/06
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Overview
The GenTox 2 micronucleus analysis/colony counting analyzer is designed specifically for toxicology laboratories, consisting of a micronucleus analysis system and a colony counting system, to efficiently achieve digital analysis of mammalian red blood cell micronucleus tests and bacterial recovery mutation tests. For economic considerations, the micronucleus imaging of GenTox 2 was achieved by modifying the existing three eye microscope in the laboratory.
Product Details
GenTox 2Micronucleus analysis/colony counting analyzer
GenTox 2Micronucleus analysis/colony counting analyzerSpecially designed for toxicology laboratories, consisting of a micronucleus analysis system and a colony counting system, it efficiently realizes digital analysis of mammalian red blood cell micronucleus test and bacterial recovery mutation test.
For economic considerations, the micronucleus imaging of GenTox 2 was achieved by modifying the existing three eye microscope in the laboratory.
Intelligent analysis system for red blood cell micronucleus
Economic universality
Using the existing microscope in the laboratory, a high-sensitivity CCD camera is connected to the microscope through a C-type adapter, making it a color digital microscope. According to the national standard for red blood cell micronuclei, color imaging analysis was performed based on Giemsa staining.
Automatic calculation of micronucleus cell rate
Through deep learning of PCE and NCE cells and random resonance image processing, the proportion of PCE in total red blood cells was determined within 20 seconds; Within 60 seconds, 2000 PCE cells were captured from 200 different field of view micrographs, and the micronucleus cell rate was automatically identified and calculated, greatly improving the efficiency of microscopic examination.
Image database management, convenient human-machine operation
1. Review and retest: For identified cells, quick follow-up and localization can be carried out to retrieve the original images and coordinates.
2. Quickly establish a sample image library: five groups (high, medium, and low dose groups, positive and negative control groups), 50 glass slides, and 10000 photos (one micrograph represents one field of view) for easy retrieval at any time.
Data Security and Audit Tracking
1. Multi account management: Administrators comprehensively manage operator accounts, passwords, account freezing, etc., to prevent data leakage or tampering between multiple operators.
2. Adopting audit trail technology, the system
Internal records: personnel identity, operation process of each operator, including time, samples, whether statistical results have been modified, whether historical data has been deleted, and all historical files.
Internal records: personnel identity, operation process of each operator, including time, samples, whether statistical results have been modified, whether historical data has been deleted, and all historical files.Colony counting system
Fully enclosed scene lighting, high pixel camera, highlighting tiny bacterial colonies
Three color LED mixed soft light box, Crystal Sharp suspended dark field illumination, high fidelity fixed focus lens, 16 megapixel camera, sharp display of colony color and contour, providing image assurance for Ames test accuracy.
Security architecture design to achieve effective audit tracking
The four tiered system architecture of "system administrator, data administrator, operator, and reviewer" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information.
System administrator: responsible for creating and managing accounts for all operators and auditors
And login password.
And login password.Data administrator: responsible for archive management, sealing, and computer database management of all test data.
Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies
Auditor: Responsible for reviewing the test reports submitted by operators.
One click intelligent six modes
To facilitate rapid and accurate colony counting by experimenters, six intelligent modes have been optimized and designed based on common plate types:Flat sense mode, three-dimensional sense mode, small colony priority, large colony priority, same color bacteria priority, medium exclusion modeComplete colony counting with just one click.
Main functions and technical indicators of the instrument
1、 System composition
1 GenTox 2 host
Intelligent analysis software for red blood cell micronuclei; Colony analysis software; 1 encryptor
Lenovo all-in-one computer (nationwide warranty): dual core CPU/4G memory/1T hard drive/21.5 'color display, Windows 7 or Windows 10
Microscopic Color CCD Camera
2、 Microscopic CCD camera
Research grade color CCD large array camera
Sensor model/size: Sony ExView HAD CCD chip 1.4M/ICX285AQ (C); 2/3 inch
Pixel: 6.45X6.45 μ m
G light sensitivity, dark current: 1240mV with 1/30s; 10mv with 1/30s
FPS/Resolution: 15@1360x1024
Exposure time: 0.12ms~240s
Data interface: USB 2.0
3、 Micronucleus analysis software
1. Fast image acquisition
CCD connection: enables real-time dynamic observation of ultra large field of view microscopic images, reducing the amount of images captured.
CCD adjustment: It has the functions of adjusting exposure time and white balance
CCD photography: Microscopic image acquisition, automatic saving of batch images
2. Cell feature learning
Positive red blood cell learning: Randomly selecting typical mature red blood cells (NCE), intelligent learning, and memory cell characteristics
Polychromatic red blood cell learning: Randomly selecting typical immature red blood cells (PCE), intelligent learning, and memory cell characteristics
Revise selected cells: with undo, clear and reselect functions
3. Experimental parameter settings:Total number of observed red blood cells, number of observed polychromatic red blood cells
4. Analyze parameter adjustment:Resonance total intensity, affinity diffusion, micronucleus sensitivity
5. PCE and NCE analysis:Complete automatic recognition and capture of PCE and NCE in 20 seconds; Automatically calculate PCE/RBC
6. Micronucleus analysis:Complete PCE capture and intelligent recognition of micronuclei containing cells in 60 seconds; Automatic calculation of micronucleus cell rate
7. Information tracing:The detected PCE cell array is digitally located, and images and coordinates are recorded for follow-up verification of cell recognition accuracy
8. Data management:
Electronic records: record the operator's experimental data to ensure data accessibility and integrity;
Report output: Output in "PDF" or "EXCEL" format, the output report data is consistent with electronic records and cannot be changed.
Account management: graded management by administrators and operators, only authorized personnel can log in; Administrators comprehensively manage operator accounts, passwords, account freezing, etc.
Audit tracking: Record personnel identities, operational processes of each operator, including time, samples, whether statistical results have been modified, whether historical data has been deleted, and all historical records.
4、 Colony digital imaging host
1. Light source
Visible light: High brightness three color LED structured light
254nm UV: used for cavity disinfection
2. Light path and lighting control
Fully enclosed dark box: eliminating environmental stray light interference
Upper light source: Scene style 360 ° flexible shadowless lighting
Lower light source: Crystal Sharp floating dark field illumination
Up light, down light, dual light, UV, freely switchable, adjustable light intensity
3. Optoelectronic conversion
High definition industrial fixed focus lens: focal length 8mm, resolution 150lp/mm
Professional CMOS camera: 1/2.33-inch color CMOS sensor, 16 million pixels C-Mount、USB3.0
5、 Colony counting software
1. Basic colony counting function
Type of petri dish: pouring, coating, membrane filtration, spiral petri dish, 3M paper sheet, porous plate
One click intelligent counting (6 modes): flat sensing mode, three-dimensional sensing mode, small colony priority, large colony priority, same color bacteria priority, culture medium removal mode
Whole dish colony count: Count the total number of colonies and display them classified by 25 sizes
Region selection statistics: You can choose circular, rectangular, or arbitrarily delineated regions for statistics
Diameter classification statistics: Set a diameter range and count colonies of specific sizes
Mouse click statistics: Quickly mark and add colonies, suitable for counting colonies at the edge of culture dishes
Colony adhesion segmentation: Automatically segment colonies that adhere to each other, and users can choose to segment or not segment chain colonies
2. Advanced colony counting function
Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain the best statistical effect.
Deviation estimation statistics: suitable for situations with multiple and complex colony colors.
Level set multi model algorithm: search operation, obtain the best image segmentation effect, adapt to background changes in the culture medium
Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics
Trans statistics: suitable for extremely complex colony types and uniform culture medium background
3. Advanced tools
Manual counting correction: adding or deleting colonies
Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically remove the bacterial count from the contaminated area
Background text elimination: automatically eliminates marker interference
Artificial Adhesive Segmentation: Manually segmenting multiple adhesive colonies
Automatic parameter conversion: Input the diameter of the culture dish and sample dilution to achieve automatic conversion
Text and graphic annotations
4. Calibration and measurement
Instrument calibration: The instrument comes with built-in calibration and manual correction calibration
One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes
Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony
Manual precise measurement: length, angle, radian, area, arc, any curve
6、 Data Security and Management
1. The four tiered system architecture of "system, data, operation, and review" is divided into functions and permissions to ensure the security, integrity, and authenticity of data information
System administrator (top-level): responsible for creating and managing accounts and login passwords for all operators and auditors. Ensure account and data isolation between operators and between operators and auditors.
Data Administrator (Deputy Senior): Responsible for archive management of all test data and database management of computers. Seal all approved test reports or backup and export original images and test data to ensure data integrity and security.
Operator: Responsible for testing, self checking, correcting, generating electronic reports, submitting for review, and printing reports on approved documents for culturing dish colonies.
Reviewer: Responsible for reviewing the test reports submitted by operators. Verify the data input and processing process, but have no authority to modify it; Review and return the suspicious report, and request the operator to retest it; The "approved" report will be archived, and neither the auditor nor the operator has the right to delete it again to ensure the originality and authenticity of the data.
2. Data storage and export
Mainly based on electronic data, record: sample source, number, dilution, petri dish image, recognition effect, count value, statistical tools used, parameter settings, correction status, to ensure complete record information.
Meet quality audit requirements, and the stored electronic data can be printed and output in PDF or Excel format
3. Watermark signature technology, tamper proof technology, and intelligent reconstruction technology for testing processes to achieve effective audit tracking
4. Anti tampering technology
Adopting multi-user login management, the names of all operators and auditors are automatically recorded in the operation process and test report by the system; All operation dates and review dates are automatically generated by the computer to avoid errors or forgeries.
The entire operation process, including colony images, dish size, sample dilution, statistical tools, parameters used, total bacterial count obtained from testing, self checked and corrected total bacterial count, etc., is automatically recorded in the database by the computer and cannot be modified by the operator, providing all real data for subsequent audits.
5. Watermark signature technology
The test report that has been "approved" will automatically generate electronic signatures for the operator and auditor's accounts, and an anti-counterfeit "approved" watermark will be printed on the report.
6. Intelligent refactoring technology for testing process
The "reviewer" opens the "waiting for review" test record, and the computer automatically restores the entire process and testing environment of the operator, including: images of the culture dish measured at that time, test results, dish size, sample dilution, statistical tools and parameters used, total bacterial count obtained from the test, correction status
By reproducing the testing environment and testing process, the reviewer can trace all the operations of the operator, verify the accuracy of the test results, and achieve the purpose of audit tracking.
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