Czone 12 AI intelligent colony counting and inhibition zone measuring instrument

Acts 12 AIIntelligent colony counting and antibacterial zone measuring instrument

Czone 12 is a colony counting and inhibition zone measuring instrument produced by Xunshu, embedded with AI modules. It is designed specifically for large and medium-sized enterprise testing institutions, and can meet the quantitative analysis of microorganisms in food, pharmaceutical, environmental, water quality, and antibacterial related industries.

Exquisite all metal design, sturdy and fast heat dissipation, the instrument has three built-in lighting paths: compound floating dark field lighting, color transparent bottom lighting, and fog diffuse reflection lighting. The high-definition fixed focus lens matched with a high pixel industrial camera can obtain sharp, finely colored, and high-resolution digital images for both colony analysis and antibacterial zone measurement.

Rich software configuration: AI colony counting software, fast and easy to use, can adapt to various complex tablets, with high precision and robustness; The antibacterial zone and antibiotic potency software can accurately detect the boundary of the transparent zone, ensuring the accuracy and repeatability of experimental data.

Multi mode petri dish imaging system

The petri dish loading chamber can realize the cultivation of petri dishesMist diffuse reflection lighting, floating dark field lighting, color (5 colors) transparent backlight lightingLight source controllertool5Road color background selection5Road lighting switch3Channel stepless brightness adjustment, single channel color temperature adjustment.

digital imaging

The Czone 12 uses a 12mm 10 megapixel high-performance fixed focus lens (with a resolution of up to 200lp/mm), and when the aperture is F4, it can not only capture dark colonies excellently, but also adapt to changes in depth of field when the culture dish is inverted.

20 million pixel, 1/1.8 'CMOS camera with bright and clear imaging, capable of high-resolution display of edge details of bacterial colonies or inhibition zones.

High speed and convenient AI colony counting

The Czone 12 AI intelligent colony counting system is easy to operate, without the need to select algorithms or parameters. With just one click, it can start analysis and continuously capture petri dishes for batch analysis of colony images,Count 200 petri dishes within 180 seconds.

AIArtificial intelligence high-precision automatic colony counting

The broad representativeness and effectiveness of big data are important foundations for determining the accuracy of AI. The Xunshu AI colony analysis system, based on 20 years of promotion and accumulation of massive user colony images, has been trained with high computing power through neural networks to form a fast, stable, and high-precision large model. It can not only accurately count conventional petri dishes, but also achieve accurate counting of dishes with strong adhesion and high interference from impurity backgrounds.

Mixed colonies with significant differences in morphology and size under dark field of view

The color of small bacterial colonies is similar to that of turbid culture medium

Different depths of bacterial colonies on the filter membrane

Various colored colonies with glossy opaque culture medium

Measurement of inhibition zone and determination of antibiotic potency

The key to automatic recognition of the antibacterial zone is to accurately find the "boundary line" between the antibacterial transparent area and the underlying bacteria. The suspended dark field system designed by Xunshu uses an LED bead array with a lifespan of up to 100000 hours and side backlighting, creating a "black background" with a transparent antibacterial zone and creating a high contrast with the surrounding gray white bacterial layer, greatly enhancing the contrast of the image.

ZoneMulti mode antibacterial zone measurement algorithm, suitable for measuring different types of antibacterial zones

The edge of the antibacterial zone is clear and rounded - "automatic detection" algorithm

Fine serrated edge of antibacterial zone - "pseudo circle approximation" algorithm

Fuzzy edge of antibacterial zone - Three point circle "manual detection"

Antibiotic potency symbolAccording to the Chinese Pharmacopoeia2025Version two dose method and three dose method

Main functions and technical indicators

1lighting system

Ø Fully enclosed steel aluminum alloy chassis (32 × 34 × 46cm): precise, sturdy, ensuring light tightness

Ø Petri dish sample compartment: Pull down aluminum alloy partition window eliminates environmental stray light interference, blocks UV leakage, and prevents dust from entering

OColor (5 colors) transparent backlight illumination

1) Adjustable LED light guide array, forming uniform and bright 5 color transmitted light

2) Illumination uniformity greater than 90%, ensuring uniform illumination at the edges and center of the culture dish

OFog gradient diffuse reflection illumination

1)ringformLEDBy using nanomaterials for light guidance and uniform light diffusion, a large area of foggy diffuse illumination is formed

2)selfDisplaying the color, texture, and transparency of bacterial colonies without shadows, eliminating the light spots and halos formed by the refraction of glass petri dishes

OCompound floating dark field illumination

White LED and blue LED interweave and mix, broadband retroreflection, forming a cosmic blue background

OUV disinfection: High power deep ultraviolet UVC-LED, instant sterilization, does not produce ozone, stable light efficiency, long lifespan

OLight source controller

Invisible suction control panel, 5Road lighting selection switch3Channel stepless brightness adjustment

Free switching and selection of single mode lighting or combined mode lighting

IIdigital imaging

Ø Fixed focus lens: 12mm, 10 mega pixel, 200 lp/mm, F1.8~F32, C-Mount

Ø Color industrial camera: 1/1.8 'color CMOS, resolution of 20 million pixels, single pixel size 1.4X1.4um

IIIColony analysis module

1. AIIntelligent colony counting

Suitable for various types of petri dishes and test pieces: pouring, coating, membrane filtration, contact dishes, 3M test pieces

Ø Fully automatic AI intelligent colony counting, one click start, no parameter setting required

OHigh throughput rapid counting, capable of batch analysis of 200 petri dishes within 180 seconds

Statistical accuracy: The counting accuracy of standard strains reaches over 98%; A plate with a colony count of less than 300 CFU and no spreading colony growth can achieve a counting accuracy of over 95%

Ø Verification eye: flashing switch mark to verify colony recognition accuracy

AI technology solves common difficult samples: segmentation of long chain colonies; Interference from water mist and label text; Changes in the color, translucency, dryness, and uneven thickness of the culture medium; Bacterial colonies vary in size, depth, morphology, and high adhesion; Background interference from impurities and slightly soluble substances

Ø Sample registration, data processing, report generation and export

2. Advanced colony statistics

Monochrome classification statistics: Select specific bacterial colonies based on color accuracy, diffusion, colony size, and contour features

Dynamic adjustment statistics: The statistical results can be dynamically adjusted and corrected to quickly obtain the best statistical effect.

Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

Ø Level set multi model algorithm: search operation, obtain the best image segmentation effect, adapt to background changes in the culture medium

Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

Trans statistics: suitable for extremely complex colony types and uniform culture medium background

Ø Removal of bacteria and impurities: Automatic removal of bacteria and impurities based on differences in morphology, size, and color

4AI Data Management

Having operational permissions and user login management, task management, audit tracking management, data information management, and other functions, ensuring the authenticity, accuracy, completeness, and traceability of data.

1. Operation permissions and user login management

The four tiered system architecture of "system administrator, data audit administrator, experimental operator, and experimental reviewer" is divided into functions and permissions.

Ø System administrator: Create accounts for all operators, reviewers, and auditors, and define user role permissions. Each user must use an independent username and password to log in, ensuring the traceability of logged in users and ensuring personal data isolation.

Ø Experimental operator: Establish engineering documents, register sample information, complete colony counting, generate electronic data, and submit it to the reviewer. If the review fails, the operator needs to perform the experimental operation again.

Ø Experimental reviewer: Conduct a comprehensive review of statistical data and verify the accuracy of counting analysis. Do not have the authority to delete or modify generated data.

Ø Data Review Administrator: Review all test data and seal all approved engineering files, including images and test reports. Responsible for data backup and recovery, preventing deletion and tampering, ensuring data integrity and security.

2. Data Information Management

Ø The electronic data generated by the analysis system is saved, backed up, restored, and retrieved in the form of engineering files.

Ø All engineering file data is deeply hidden to prevent illegal deletion, modification, or theft, ensuring the security of the files.

Ø The electronic data within a single engineering file consists of sample registration information, statistical result information, test reports, and a set of petri dish images of batch labeled colonies. Sample registration includes: sample name, number, report name, testing unit, sending unit, testing personnel, and testing date. The statistical results include: dish number, dish name, number of identifications, dilution factor, and total conversion. The test report can bePDForExcellFormat output.

Ø Data protection: The system can back up original engineering file data, audit trail data, and original images to external media, or restore files from external media to the system. Transmission is verified to ensure lossless data.

Ø Data retrieval: You can quickly search for historical files by time period or name, and then select them with just one click to import and comprehensively display the historical operation status.

3. Task Management

Ø Display the names, creation times, and current status of all engineering file projects (such as new file, submitted for review, and approved review)/Return), one click file switching, quick and direct access.

Ø Each engineering document adopts a step-by-step approval process, including submission, approval, or return. The data that has been approved for review is frozen and cannot be modified again; The data returned for review needs to be verified and redone.

4. Audit tracking management

Ø Audit tracking can record in detail the login, logout, creation of new projects, import of images, opening, closing, and saving of projects by various identity operators in a timeline.

Ø After the instrument is running, the audit trail can be automatically turned on and cannot be turned off, presented in an open and readable form.

Ø The audit trail content cannot be deleted or modified, and can be viewed or exported using a timeline or project name

5Antibacterial zone analysis module

1. ZoneMulti mode measurement technology for antibacterial zone

Ø Automatic detection: precise edge detection based on the contour of the antibacterial zone, suitable for clear edges and circular antibacterial zones

Ø Circular approximation: Based on the contour of the antibacterial zone, circular approximation is suitable for edge rupture and non-standard circular antibacterial zones

Ø Manual detection: Click on three points on the edge of the antibacterial zone with the mouse to form a circle, suitable for antibacterial zones with blurred edges

2. Antibiotic potency determination

Ø One dose method potency testing: suitable for the United States Pharmacopeia

OTwo dose method, three dose method, and combined calculation: suitable for the Chinese Pharmacopoeia2025edition

Ø Repetitive self check

Ø Uniformity self-test

Ø Inter station measurement difference ≤ 0.2%

VIInstrument specifications and configuration

Ø Czone 12 AI colony counting and inhibition zone measuring instrument host 1 unit

OAIColony counting software, classic colony analysis software, automatic inhibition zone measurement software, antibiotic potency determination software

OBrand computer:i5 CPU/16Gmemory/1Thard disk+512G SSD/integrated graphics/27'HD screen,Windows 11system