Intelligent colony counter

HD2000（AI）Intelligent colony counter

HD2000(AI) is a high-quality AI that will be fully upgraded and integrated with neural network technology by Xunshu Technology in 2024Intelligent colony counterIt is easy to operate and can quickly and batch solve the colony counting of various petri dishes.

Digital imaging consists of a high-resolution large target fixed focus lens and a 1-inch large area array CMOS sensor, providing clear and sharp image quality, bright and delicate colors. Especially for small psychrophilic bacteria, mycoplasma, microbial test strips, etc., it has good discrimination and recognition ability.

The instrument adopts a delicate, sturdy, and well cooled all metal architecture design, with rich lighting paths: high contrast suspended dark field illumination, fog gradient diffuse illumination, oblique backlight illumination, quasi infrared thin-layer interference illumination, dual light path dual wavelength staining illumination.

The pseudo infrared thin-layer interference illumination can display tiny, gray white colonies on the filter membrane in a three-dimensional relief effect, especially suitable for the membrane filtration method of drug microbiological limit testing.

l Multi mode professional image shooting system

Composition of the Petri dish sample chamberHigh contrast suspended dark field illumination, fog gradient diffuse illumination, transit backlight illumination, quasi infrared thin-layer interference illumination, dual path dual wavelength staining illuminationBy adjusting the brightness and light ratio, take rich and contrasting beautiful pictures.

l High definition digital imaging

24 million pixel high-resolution 1.1-inch fixed focus lens, SONY 20 million pixel 1-inch ultra large array

CMOS camera, sharp display of bacterial coloniesForm, color, glossiness, texture, and edgesfeature

l Quasi infrared thin-layer interference illumination (filter membrane specific)

Traditional bright field lighting, due to the close grayscale level between light white colonies and white filter membranes, makes it difficult to observe colonies and may result in missed counts.

Xunshu's "pseudo infrared thin-layer interference illumination" technology constrains the sheet-like beam to irradiate the colony along the surface of the filter membrane, creating a brightness difference and making the colony appear in a clear relief shape. Even extremely small or flat colonies can be visible.

l Light staining effect (dual path dual wavelength)

When the color of the colony and the filter membrane are close, there are difficulties in intelligent recognition and counting. Illuminate two different wavelengths of light at different angles to achieve light staining of colonies and filter membranes in the culture dish, resulting in two different colors of colonies and filter membranes, which helps to improve the accuracy of colony recognition and counting.

l Simple and efficient counting method

HD2000 AI operation is minimalist, with one click to start analysis. Users do not need to select algorithms or parameters, and can continuously capture petri dishes for batch analysis and counting of colony images,Count 300 petri dishes within 240 seconds.

l AI achieves high-precision colony counting with artificial intelligence

The broad representativeness and effectiveness of big data are important foundations for determining the accuracy of AI. The Xunshu AI colony analysis system, with 20 years of promotion of the classic colony analyzer, has accumulated a massive amount of user colony images. Through neural network training, it has formed a high-speed, high-precision, and stable large model.

Case study of AI colony recognition and counting

Mixed circular, shuttle shaped, and small bacterial colonies

Spread mold under dark field of view

Small water droplets on the lid of the culture dish affect the clarity of colony imaging

Uneven thickness of agar creates interference between light and dark backgrounds

Micro porous filter membrane for infrared thin-layer interference imaging

Interference of bottom text under backlight illumination

Grey white aerobic bacteria mixed with brown traditional Chinese medicine powder

Main functions and technical indicators

1 lighting system

Ø Fully enclosed steel aluminum alloy chassis (32 × 34 × 46cm): precise and sturdy, ensuring a light tight seal

Ø Flat dish sample compartment: aluminum alloy frame, pull-down partition window, eliminates environmental stray light interference, blocks UV leakage, and prevents dust from entering

Ø Quasi infrared thin-layer interference illumination

l draftContinuous adjustable tilt angle of infrared thin-layer light, embossed display of bacterial colony uplift state

l Suitable for filter membrane petri dishes, flat, light colored, and clearly visible small bacterial colonies

Ø High contrast floating dark field illumination

Circular layered LEDBroadband retroreflectionHighlight the outline and color of bacterial colonies in the dark field, and keenly capture micro colonies on the surface and deep layers of the culture medium

Ø Color transparent backlight illumination

l Adjustable LED light guide array, forming uniform and bright 5 color transmitted light, enhancing the contrast between bacterial colonies and culture medium

l Illumination uniformity greater than 90%, ensuring uniform illumination at the edges and center of the culture dish

Ø misty lightgradientDiffuse reflectance lighting

l Circular LED forms a large area of fog diffuse illumination through the guidance and uniform light diffusion of nanomaterials

l Natural display of colony color, texture, transparency, no shadows on colonies, eliminating light spots and halos caused by glass plate refraction

Ø Light staining illumination (dual path dual wavelength)

Two wavelengths of light are irradiated at different angles on the petri dish to achieve light staining of colonies and culture media (filter membranes), improving the accuracy of colony recognition and counting.

Ø UV disinfection: High power deep ultraviolet UVC-LED, instant sterilization, does not produce ozone, stable light efficiency, long lifespan

Ø Light source controller

l concealShaped suction control panel,5Road lighting selection switch3Channel stepless brightness adjustment

l CanSwitch and select single mode lighting or combined mode lighting freely

II digital imaging

Ø 1.1-inch large target fixed focus lens: 24 million pixels, resolution of 200 lp/mm, F2.4

Ø Color industrial camera: SONY 1 "color CMOS sensor, resolution of 20 million pixels, single pixel size of 2.4X2.4um, G light sensitivity of 462mv with 1/30s

III Colony analysis module

1. AI intelligent colony counting

Ø Suitable for various types of petri dishes and test pieces: pouring, coating, membrane filtration, contact dishes, 3M test pieces

Ø Fully automatic AI intelligent colony counting, one click start, no parameter setting required

Ø Rapid statistics, single dish colony counting only takes 0.8 seconds; High throughput counting, capable of batch analysis of 300 petri dishes at once

Ø Statistical accuracy: The counting accuracy of standard strains reaches over 98%; A plate with a colony count of less than 300 CFU and no spreading colony growth can achieve a counting accuracy of over 95%

Ø Verification eye: flashing switch mark to verify colony recognition accuracy

Ø AI technology solves common difficult samples: segmentation of long chain colonies; Interference from water mist and label text; Changes in the color, translucency, dryness, and uneven thickness of the culture medium; Bacterial colonies vary in size, depth, morphology, and high adhesion; Background interference from impurities and slightly soluble substances

Ø Sample registration, data processing, report generation and export

2. Advanced colony statistics

Ø Dynamic adjustment statisticsThe statistical results can be dynamically adjusted and corrected to quickly obtain the best statistical results.

Ø Deviation estimation statistics: suitable for situations with multiple and complex colony colors.

Ø Level set multi model algorithm: search operation, obtain the best image segmentation effect, adapt to background changes in the culture medium

Ø Specific colony statistics: Identify specific colonies based on their color, size, and contour characteristics

Ø Trans statistics: suitable for extremely complex colony types and uniform culture medium background

Ø Statistics of high adhesion bacteria: segmentation calculation suitable for multiple adhesion bacteria

Ø Removal of miscellaneous bacteria and impurities: Automatic removal of miscellaneous bacteria and impurities based on differences in morphology, size, and color

3. Typical bacterial screening

Ø Monochromatic classification statistics: Select specific bacterial colonies based on color accuracy, diffusion, colony size, and contour features

Ø Multi color automatic clustering: automatically distinguish 24 different colored colonies based on color clustering accuracy

Ø Multi color screening: screening 1-8 color colonies at once

Ø Transparent circle characteristic analysis: suitable for antibacterial circle, hydrolysis circle, discoloration circle, calcium dissolution circle, hemolysis circle, oil discharge circle, phosphorus dissolution circle analysis

Ø Dual color circle automatic filtering

4. Description of colony characteristics

Ø Bacteria and yeast: features such as color, size, shape, surface morphology, edge, gloss, transparency, etc., intelligently described and sorted

Ø Mold and actinomycetes: features such as front color, back color, size, surface morphology, edges, texture, etc., intelligently described and sorted

5. Microbial limit analysis tool

Ø Applicability check of culture medium

Ø Control bacterial examination - colony morphology

6. specialized analysis

Ø Mold proof testing: quantitative analysis of mold proof level

Ø Multi region serial statistics: suitable for complex bacterial colonies with uneven background in the culture medium

Ø Multi region parallel statistics: suitable for porous plate, OPKA, SBA analysis

7. Calibration and Measurement

Ø Instrument calibration: The instrument comes with built-in calibration and manual correction calibration

Ø One click rapid measurement: One click determination of large bacterial colonies, suitable for single colony analysis of fungi and actinomycetes

Ø Automatic measurement of the entire dish: analysis of the equivalent diameter, area, length, circumference, and roundness of the entire dish colony

Ø Multi directional ruler measurement, manual precise measurement: length, angle, radian, area, arc, arbitrary curve

8. image processing

Ø Image adjustment: grayscale image and negative phase diagram conversion; Brightness, contrast, and saturation adjustment; RGB adjustment

Ø Image enhancement: sharpening, adaptive enhancement

Ø Image filtering: median filtering, high pass filtering, Gaussian filtering, low-pass filtering, queue filtering, high pass Gaussian filtering

Ø Edge detection: Sobel operator, Robert operator, Laplace operator, vertical detection, horizontal detection

Ø Morphological operations: erosion, dilation, opening operation, closing operation

4 Advanced Electronic Recording and Data Security Management with AI

Having operational permissions and user login management, task management, audit tracking management, data information management, and other functions, ensuring the authenticity, accuracy, completeness, and traceability of data.

1. Operation permissions and user login management

The four tiered system architecture of "system administrator, data audit administrator, experimental operator, and experimental reviewer" is divided into functions and permissions.

Ø System administrator: Create accounts for all operators, reviewers, and auditors, and define user role permissions. Each user must use an independent username and password to log in, ensuring the traceability of logged in users and ensuring personal data isolation.

Ø Experimental operator: Establish engineering documents, register sample information, complete colony counting, generate electronic data, and submit it to the reviewer. If the review fails, the operator needs to perform the experimental operation again.

Ø Experimental reviewer: Conduct a comprehensive review of statistical data and verify the accuracy of counting analysis. Do not have the authority to delete or modify generated data.

Ø Data Review Administrator: Review all test data and seal all approved engineering files, including images and test reports. Responsible for data backup and recovery, preventing deletion and tampering, ensuring data integrity and security.

2. Data Information Management

Ø The electronic data generated by the analysis system is saved, backed up, restored, and retrieved in the form of engineering files.

Ø All engineering file data is deeply hidden to prevent illegal deletion, modification, or theft, ensuring the security of the files.

Ø The electronic data within a single engineering file consists of sample registration information, statistical result information, test reports, and a set of petri dish images of batch labeled colonies. Sample registration includes: sample name, number, report name, testing unit, sending unit, testing personnel, and testing date. The statistical results include: dish number, dish name, number of identifications, dilution factor, and total conversion. The test report can be output in PDF or Excel format.

Ø Data protection: The system can back up original engineering file data, audit trail data, and original images to external media, or restore files from external media to the system. Transmission is verified to ensure lossless data.

Ø Data retrieval: You can quickly search for historical files by time period or name, and then select them with just one click to import and comprehensively display the historical operation status.

3. Task Management

Ø Display the names, creation times, and current status of all engineering file projects (such as new file, submission for review, review approved/returned), with one click file switching for quick access.

Ø Each engineering document adopts a step-by-step approval process, including submission, approval, or return. The data that has been approved for review is frozen and cannot be modified again; The data returned for review needs to be verified and redone.

4. Audit tracking management

Ø Audit tracking can record in detail the login, logout, creation of new projects, import of images, opening, closing, and saving of projects by various identity operators in a timeline.

Ø After the instrument is running, the audit trail can be automatically turned on and cannot be turned off, presented in an open and readable form.

Ø The audit trail content cannot be deleted or modified, and can be viewed or exported using a timeline or project name

5 Instrument specifications and configuration

Ø 1 HD2000 (AI) host

Ø AIColony counting software, classical colony analysis software

Ø Brand computer:i5 CPU/16Gmemory/1Thard disk+512G SSD/2Gdedicated graphics card/27'HD screen,Windows 11system