MicroRNAs (miRNAs) are generally difficult to detect due to their short length. The miRNA qPCR Detection System is a specialized system developed by GeneCopoeia in the United States for miRNA quantitative detection. The system includes: miRNA detection kit: upstream primers for qPCR detection of miRNAs specific to polyA, miRNA reverse transcription, and qPCR reaction
working principle:
Product features:
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Easy, fast, and cost-effective detectionThe miRNA qPCR Detection System is a qPCR detection system based on the SYBR Green dye method, which can be used to detect multiple miRNAs from a synthesized cDNA reaction. It not only reduces errors and saves samples, but also achieves high-throughput detection
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Specificity of detection:*The miRNA primer design technology and optimized reaction system avoid non-specific amplification, especially Pre miRNA contamination
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High detection resolution:This system can distinguish miRNAs with single nucleotide differences
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One stop solution:The company not only provides testing kits, but also offers validated human, mouse, and rat specific miRNA detection primers.
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High detection sensitivity:MiRNAs with specific expression can be detected in total RNA as low as pg level
Quality Description
- All-in-One ™ The miRNA qRT PCR Detection Kit already includes all the reagents required for detection, and customers only need to provide RNA and its All in One ™ MiRNA qPCR Primer can be used for miRNA detection
- The All in One we offer ™ MiRNA qPCR Primer Recommended to be Same as All in One ™ Used in conjunction with miRNA qRT PCR Detection Kit
- We promise to launch every All in One ™ MiRNA qPCR Primers are available using All in One technology ™ The report for miRNA qRT PCR Detection Kit validation includes the validation process, primer amplification curves, melting curves, electrophoresis result graphs, and recommended reaction conditions. It also provides a positive cDNA validation template, which customers can refer to for testing experiments.
Articles published by customers using this product:
Sweeney TE, Suliman HB, Hollingsworth JW, Piantadosi CA, 2010 Differential Regulation of the PGC Family of Genes in a Mouse Model of Staphylococcus aureus Sepsis. PLoS ONE 5(7): e11606. doi:10.1371/journal.pone.0011606