Merieux API50CHL Lactobacillus Identification Kit

Merieux API50CHL Lactobacillus Identification Kit

Packaging specifications10 tests/box

Testing principleAPI 50 CHLIt is used for lactobacilli(Lactpbacillus)And related bacteriaAppraisal, it is composed of49 Cultivate fermentable carbohydratesAPI 50 CHtryA simple culture medium composed of test strips. Prepare suspension inoculation test strips for measuring bacteriaEvery small tube. During cultivation, due to the fermentation of carbohydrates to produce acid,pHDecrease, causing the indicator to change color. The result constitutes the biochemical profile of the strain,And used for identification or typing.

Composition of reagent kit

API 50CHtest strip 10 Article

Cultivation box 10 A

Test Report10 Zhang

API 50CHLProduct Manuala copy

API 50CHLOperation guide carda

Suspension liquid（2mL）10 ampoules

Lactobacillus culture medium（50CHL Medium）10 ampoules

paraffin oil 3 Bottle

2 Mai's turbidity tube 1 bottle

Composition and storage conditions of test stripsAPI 50CHBThe test strip is within its validity period (indicated on the reagent kit),Should be stored in2-8℃.

Testing method

1. strain cultivation

Check the purity of the bacterial strain

Cultivate the strain on a suitable solid culture medium. If the strain grows too muchSlowly, two plates should be used for cultivation to ensure sufficient bacteriaQuantity is used for the following operations.

2. Preparation of test strips

Prepare a culture box (tray and lid), pour in about10mldistillationWater (excluding other impurities orCl2，CO2 Volatile gas waterCreate a wet chamber in the honeycomb of the tray.

Write the strain number on the edge of the plate(Do not write on the cover to avoid misplacement).

Remove from the packaging bag2 A test strip（0-19，20-39）Then, take it againThey are divided into4 A small test strip（0-9，10-19，20-29，30-39）AndAll placed in the incubation box chassis. Take out another small test strip（40-49）Place the above in the incubation box chassis4 After one test strip, the test is completedPreparation of the article.

3. Preparation of inoculum

Collect bacterial strains from solid culture medium using cotton swabs and add them to the suspensionPrepare high concentration bacterial suspension in the floating liquid（S）.

to go0.85%NaCl（5mL）Add high concentration bacterial suspension to the medium（S），Prepared with turbidity equivalent to2McFBacterial suspension, record the number of dropsn.

open50CHLCulture medium ampoule, add2nInoculate with the above-mentioned bacterial solution.

The prepared bacterial suspension should be used immediately.

4. Test strip vaccination

Add the bacterial suspension onto the test strip using a sterile sampler according to the following requirements50 aIn the management:

Gently tilt the incubator chassis forward.

Place the sampler head against the edge of the cup and add bacterial suspension to avoid generating gasBubble.

Fill only the part of the tube, do not add bacterial suspension to the upper part (cup) of the tube,This way, an anaerobic environment can be maintained inside the pipe

After the inoculation of the bacterial suspension is completed, the liquid level should avoid crescent shaped indentationsTrapped or protruding.

According to the growth needs of the tested bacteria, place the test strip at an appropriate temperatureIncubate:30°C/37°Ccultivate48hor55°Ccultivate24h.If the optimal temperature is unknown, cultivate at medium temperature.

Merieux API50CHL Lactobacillus Identification Kit