Merieux API 20 CAUX Yeast Identification Kit

Merieux API 20 CAUX Yeast Identification Kit

Packaging specifications25 test/box

Testing principleAPI 20 C AUXThe test strip is composed of19 Dry powder substrate for individual assimilation determinationof20 Composed of a small cup. The small cup contains semi-solid trace culture medium for connectionOnly yeast that can use this substrate as a carbon source can grow.The measurement results are obtained by comparing with the control growth material; Appraisal result referenceAccording to the analysis chart index or identification software.

Composition of reagent kit

Main reagent:

API 20 C AUXtest strip 25 Article

Cultivation box 25 A

API Cculture medium 25Ampoule

Test Report 25 Zhang

API 20 C AUXProduct Manual 1 Share

API 20 C AUXOperation guide card 1 Zhang

Additional reagents:

Suspension liquid（2ml） 25 Ampoule

2 Mai's turbidity tube 1 bottle

Composition and storage conditions of test strips

All reagents should be stored in the dark2-8℃Until the expiration date in the environment.

All ampoules have a validity period of only one month (or packaging) after openingThe validity period is based on the shortest possible time.

Preparation of test strips

Prepare a culture box (tray and lid), pour in about5mldistilled water(Does not contain any other impurities orCl2，CO2Volatile gas water)Yu Pan'sA small honeycomb cavity creates a wet chamber.

Write the strain number on the edge of the plate(Do not write on the cover to avoid misplacement)

Take out the test strip from the packaging and place it on the tray.

Place the test strip in the culture box.

Preparation of inoculum

Open an ampoule according to the methods listed in the "Precautions" column2mlhangFloating liquid.

Use a straw or needle to pick up the isolated colonies from the agar plate of the isolateInto the suspended substrate. Suggest using fresh cultivation（18-24Hour)

Carefully grind to achieve a uniform bacterial suspension, ensuring its turbidity reaches2 The standard turbidity of the Maillard unit. The prepared bacterial suspension should be used immediately.

Open an ampouleAPI CCulture medium, take100μlThe above bacterial suspension is addedGet into it. Gently mix with a straw, taking care to avoid creating bubbles.

Test strip vaccination

Using the same straw, inject the above mixed suspension into each test hole of the test stripIn the middle. Place the top of the straw against the inner edge of the cup and add liquid to avoid deformationForm bubbles. Be careful when injecting, the surface should be flat or slightly convex (do not sink,Otherwise, the results will be inaccurate.

Cover the culture box and29℃± 2℃ cultivation48-72Hour by hour（± 6Hour)

Merieux API 20 CAUX Yeast Identification Kit