Merieux 418985 Gram negative bacterial susceptibility card AST-N335

Gram negative bacterial susceptibility card instruction manual

General Name: Gram Negative Bacterial Antimicrobial Susceptibility Card English Name: VITEK 2 AST-N334

【 Packaging Specification 】 20 tests/box

This product is used to detect the sensitivity of clinically important aerobic gram-negative bacteria to antibiotic drugs.

【 Inspection Principle 】 Vitek ® The AST drug susceptibility card of System 2 is an automated testing method based on MIC method (reports by Maclowry, Marsh, and Gerlach). The AST drug susceptibility card is a mini and simplified version for determining the minimum inhibitory concentration using the double microdilution method.

Each AST drug susceptibility card contains a control well containing only microbial culture medium. The remaining micropores contain a predetermined amount of specific culture medium and antibiotics. Before using the test bacterial suspension to hydrate the antibiotic containing culture medium in the drug sensitive card, dilute the bacterial suspension with 0.45% saline solution to the standard concentration. Fill and seal the drug susceptibility card, then load it onto the instrument incubator for automatic operation (VITEK) ® 2 60 or Vitek ® 2 XL fully automatic microbiological analysis system) or manually operated (VITEK 2 Compact fully automatic microbiological analysis system).

The instrument monitors the growth of bacteria in each well within a specified time frame (up to 24 hours for bacteria and 36 hours for yeast). After the incubation period, obtain the MIC values (or test results) of each antibacterial agent on the drug susceptibility card.

Limitations of Inspection Methods

Usage restrictionsTicarcillin/Clavulanic acid is only approved for use in Pseudomonas aeruginosa.

Before reporting the test results of all other bacteria, alternative testing methods must be performed.

Before reporting the test results of the following antibiotic/bacterial combinations, perform alternative test methods:

AmikacinBaumann Acinetobacter baumannii

 Aminomonas: Pseudomonas genus

Cefotaxime: Bordetella bronchiolitis, Hafnia beehive, Morganella genus

Imipenem: Serratia marcescens

piperacillin/Tazobactam: Serratia marcescens

When the test results of the following antibiotic/bacterial combinations obtained are resistant, alternative test methods should be performed before reporting the results:

Imipenem: Aeromonas genus

Meropenem: Aeromonas genus

Piperacillin/Tazobactam: Pseudomonas aeruginosa

Merieux 418985 Gram negative bacterial susceptibility card AST-N335

Merieux 418985 Gram negative bacterial susceptibility card AST-N335