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Shanghai Laichuang Biotechnology Co., Ltd
Japanese cherry blossom embedding agent
NegotiableUpdate on 05/06
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Overview
The Japanese cherry blossom embedding agent $nOCT frozen section embedding agent is a water-soluble mixture of polyethylene glycol and polyvinyl alcohol, which is widely used in immunohistochemistry laboratories. Its purpose is to support tissues during frozen section to increase tissue continuity, reduce wrinkles and fragmentation. Also, as the OCT mixture is water-soluble, it is soluble in water during the bleaching process, so background staining will not be added in future staining.
Product Details
Japanese cherry blossom embedding agentIntroduction:
Using OCT mixture (optim um cutting temperature compound) to pre infiltrate tissues, and then performing constant cold box sectioning to improve the quality of sectioning.
OCT embedding agent (SAKURA product) embedding process:
1. Remove the tissue block from the sucrose solution and immerse it in a 1:1 volume ratio mixture of 20g L-1 sucrose and OCT embedding agent at room temperature for 2 hours
2. Transfer to OCT embedding agent and soak at room temperature for 4 hours. Replace with a new OCT embedding agent and soak at room temperature for 6 hours
3. Place the specimen on a tray and slice it using a constant cold box slicer (usually -22 ℃) with a thickness of 10 μ m. Attach it to a pre treated glass slide and store it in a -20 ℃ freezer.
Widely serving industries such as scientific research, pharmaceuticals, biology, life sciences, education, wastewater treatment, food and beverage, electronics, light industry, mining, oil and gas, petrochemical industry, industrial instruments, environmental engineering, etc.
