High throughput DSF analyzer

NegotiableUpdate on 12/29

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Overview

High throughput DSF analyzer, also known as high-throughput DSF (differential scanning fluorescence) analyzer, is an advanced instrument widely used in fields such as biopharmaceutical research and protein stability analysis. It can monitor the conformational changes of proteins during the heating process through fluorescent dyes or endogenous fluorescent signals, and calculate their melting temperature Tm (the temperature at which folded proteins and unfolded proteins are equal).

Product Details

1、 Basic principles

DSF (Differential Scanning Fluorescence) is a method of evaluating protein thermal stability by slowly heating a sample on a fluorescence quantitative PCR instrument and detecting the amount of binding between fluorescent dyes and proteins with structural changes during the heating process. It can monitor the conformational changes of proteins during the heating process through fluorescent dyes or endogenous fluorescent signals, and calculate their melting temperature Tm (the temperature at which folded proteins and unfolded proteins are equal).

2、 Main features

High throughput: The high-throughput DSF analyzer can obtain as much information as possible in a very short amount of time. For example, the SUPR-DSF analyzer can complete stability analysis and screening of 384 samples within 60 to 80 minutes.

Low cost: Using standard 384 well plates, no special consumables or capillaries are required, with a one-time cost of only about 100 yuan.

Wide temperature scanning range: With a wide temperature scanning range of 10 ℃~105 ℃, it can cover the structural domains of multiple proteins.

High sensitivity: Using a high-sensitivity CMOS detector, it can accurately detect fluorescence changes.

Low sample usage: The sample usage is only 1020 μ L, with a concentration range of 0.05mg/ml250mg/ml, suitable for stability analysis in multiple stages.

3、 Instrument composition

The high-throughput DSF analyzer mainly consists of the following parts:

Plate reader: used to load 384 well plates and read sample information.

Excitation light source: LED excitation wavelength is usually used, such as 280nm, with an output power of 10mW.

Detection system: including spectrometer and detection system, used for detecting fluorescence signals. The wavelength range of a spectrometer is usually 310nm~420nm.

Data processing software: used to draw the curve of fluorescence intensity with temperature, and calculate the Tm value and related parameters of protein samples by fitting the Boltzmann equation.

4、 Operation process

The operation process of high-throughput DSF analyzer usually includes the following steps:

Sample preparation: Mix the protein sample with fluorescent dye and add it to a 384 well plate.

Set parameters: Set the instrument's heating rate, temperature range, and other parameters.

Start detection: Place the 384 well plate into the instrument and begin detecting the fluorescence signal.

Data analysis: Use data processing software to process and analyze the detection data, and obtain the Tm value and related parameters of the protein sample.

5、 Application Fields

High throughput DSF analyzers have a wide range of applications in multiple fields, including but not limited to:

Protein stability analysis: Evaluate the stability of proteins under different conditions, such as temperature, pH value, ionic strength, etc.

Drug screening: By using high-throughput screening technology, candidate drugs that bind to target proteins can be quickly screened.

Protein structure research: studying the structural and conformational changes of proteins, providing strong support for research such as protein crystallization.

Research on the inhibition mechanism of small molecule compounds: used for screening small molecule compound inhibitors and studying their pharmacological mechanisms.

6、 Instrument maintenance and upkeep

In order to ensure the normal operation and prolong the service life of high-throughput DSF analyzers, regular maintenance and upkeep are required. This includes cleaning the surface of the instrument, checking the working status of the light source and detection system, calibrating the instrument, etc. In addition, attention should be paid to the handling and storage conditions of the samples to avoid contamination and deterioration that may affect the experimental results.

In summary, high-throughput DSF analyzer is a powerful and easy-to-use instrument with broad application prospects in fields such as biopharmaceutical research and protein stability analysis.