Circular dichroism instrument

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Overview

The Chirascan plus circular dichroism spectrometer, also known as the circular dichroism spectrometer (CD), is an analytical instrument widely used in the fields of chemistry and biology. It is mainly used to study the three-dimensional structure of molecules with chirality, and infer their spatial configuration and conformational changes by measuring the difference in absorption of left-handed and right-handed circularly polarized light by these molecules.

Product Details

Circular Dichroism Spectrometer (CD), also known as Circular Dichroism Spectrometer, is an analytical instrument widely used in the fields of chemistry and biology. It is mainly used to study the three-dimensional structure of molecules with chirality, and infer their spatial configuration and conformational changes by measuring the difference in absorption of left-handed and right-handed circularly polarized light by these molecules.

The basic principle of a circular dichroism analyzer is based on the phenomenon of circular dichroism (CD), which refers to the difference in absorption coefficients (ε) of certain optically active substances for left-handed and right-handed circularly polarized light that make up plane polarized light. This difference causes the transmitted light to become elliptically polarized light. Specifically, when a beam of plane polarized light enters an optically active material, the material absorbs left-handed and right-handed circularly polarized light differently, resulting in the combination of their electric vectors to form elliptically polarized light. This difference can be quantified by measuring the molar ellipticity θ, and subsequently obtaining circular dichroism spectra.

It mainly consists of a light source, a sample chamber, a spectroscopic system, a detector, and a data processing system. The light source is usually a tunable laser or LED light source, providing circularly polarized light. The sample room is used to store the samples to be tested. The spectroscopic system divides the light emitted by the light source into left-handed and right-handed circularly polarized light, which are respectively irradiated onto the sample. The detector detects the absorption intensity of left-handed and right-handed circularly polarized light passing through the sample and converts the absorption intensity into an electrical signal. The data processing system converts these electrical signals into circular dichroism spectra and performs further data processing and analysis.

The application of circular dichroism is very extensive, especially in fields such as organic chemistry, biochemistry, coordination chemistry, and medicinal chemistry. It is widely used to determine the stereoisomers of organic compounds, study the conformational changes of biomolecules such as proteins and nucleic acids, and characterize chiral complexes. In addition, it can also be used for research on hydrogen bonding assembly, π - π self-assembly, and host guest molecular interactions in the field of supramolecular chemistry.

When using, attention should be paid to factors such as sample purity, solvent selection, and control of nitrogen flow rate to ensure the accuracy and reliability of test results. Meanwhile, it is also necessary to pay attention to the daily maintenance and calibration of the instrument to ensure its long-term stable operation.

In summary, circular dichroism is a powerful analytical tool that is of great significance for studying the stereostructure and conformational changes of chiral molecules.