Halo core-shell chromatography column

Halo core-shell chromatography column

Nantong Hairuo Chemical Co., Ltd

Since its establishment in 2005, Advanced Materials Technology has been committed to optimizing the presentation of samples into detectors.

With Fused Core ® Particle design creates innovative solutions for the separation science community. The quality control process and standards have been deeply integrated into every HALO delivered ® In the chromatographic column, ensure that every time is trustworthy.

Nearly sixty years ago, Horvath and Lipsky published a groundbreaking paper describing their mesh like particles made from glass particles coated with a thin layer of ion exchange resin.

This paper inspired Jack Kirkland, who was working at DuPont at the time, to develop surface porous silica gel particles with solid cores and porous shells (30 µ m Zipax introduced in 1969) ®）。 The emergence of surface porous particles has opened up the evolution of liquid chromatography technology towards modern analytical tools.

However, this type of particle was quickly replaced by the small particle fully porous silica gel particles that emerged in the early 1970s, which subsequently dominated HPLC column technology.

Until 2006, surface porous particles were reintroduced as a major breakthrough in HPLC particle technology. At that time, Kirkland, Langlois, and DeStefano from Advanced Materials Technology introduced the sub-3 µ m Fused Core ® Commercialization of silicone particles and launch of HALO ® brand

This type of particle consists of a solid silicone core and a thin porous shell wrapped around the outside. The shell is tightly bonded to the core through a hot sintering process, which is also known as Fused Core ® The reason for (fusion core/core-shell). Fused-Core ® The chromatographic column can run on HPLC and UHPLC systems and has excellent performance.

Fused-Core ® Advantages of Technology

Compared to fully porous particles (FPP), surface porous particles (SPP) have a shorter diffusion path for sample molecules entering and exiting the particles due to the presence of an impermeable solid core, resulting in a faster mass transfer rate.

2.7 µ m Fused Core ® The chromatographic column has obvious advantages, and its performance is even better than the sub-2 µ m fully porous particle (FPP) chromatographic column.

HALO ® Other advantages of chromatographic column technology

HALO ® Performance measurement data of chromatography columns and three competing chromatography columns (2.1 x 50 mm SPP and FPP). The test conditions are: flow rate of 0.5 mL/min, mobile phase of 50/50 acetonitrile/water, temperature of 35 ° C.

The results show that all HALOs ® The theoretical number of trays provided by the chromatographic column at unit pressure is higher than that of any competing sub-2 micron SPP and FPP chromatographic columns.

HALO ® Analysis of Particle Structure

HALO ® The birth of chromatographic columns began with the manufacturing of solid silica gel cores, which were produced using high-quality raw materials. As the cornerstone of particles, the core manufacturing process must be closely monitored. This primary process includes over 45 different process controls and 4 independent QC/QA tests. We implement strict tolerance standards to ensure that every core produced has a high degree of reproducibility and consistency.

The attached figure shows that there is minimal change in the 1.7 µ m core throughout the lifecycle of the product. This achievement demonstrates our commitment to our customers: using AMT driven HPLC chromatography columns to ensure long-term reproducibility of separation results.

Next, wrap a porous silicone shell around the core. In this step, AMT has control over the aperture and shell thickness, ensuring consistency and reliability of the surface area, as shown in the figure. The consistency of surface area across batches and years brings about chromatographic reproducibility.

This process includes over 100 process controls and 7 rigorous QC/QA tests, fully demonstrating how we meticulously control and monitor the thickness of porous shells.

The size of the aperture is achieved through careful selection of nanoparticles in the process. The size of the target analyte to be separated determines the choice of pore size: larger pore sizes are designed specifically for biomolecules, while smaller pore sizes are designed specifically for small molecules.

AMT has 5 different pore sizes of products: 90 Å, 120 Å, 160 Å, 400 Å, and 1000 Å.

The silicone SPP manufacturing process of AMT can achieve extremely narrow particle size distribution, which is HALO ® A significant indicator of chromatographic column quality. This narrow particle size distribution helps to manufacture high-performance chromatography columns with stable chromatographic beds. The figure shows 2.7 µ m HALO ® Example of particle size distribution comparison between particles and 3 µ m fully porous particles. The standard deviation of AMT's SPP process is more than three times lower than that of FPP manufacturing process.

Jack, the father of "core-shell" chromatographic packing technology Dr. Kirkland led his team (AMT Corporation) to launch the HALO 2, a sub 2 μ m specialized core-shell chromatography column for UPLC, in June 2014. The column is super durable, highly efficient, and has extremely low back pressure.

The HALO 2 UPLC core-shell chromatography column can effectively solve the shortcomings of the existing conventional silica gel sub-2 μ m UPLC chromatography column and has all its advantages.

1. Advantages of HALO 2 core-shell chromatography column over 2 μ m non core-shell UHPLC chromatography column

Ultra high column efficiency: HALO 2 UPLC core-shell chromatography column can provide you with an ultra-high column efficiency of 300000 theoretical plates (higher than existing non core-shell sub-2 μ m chromatography columns).

Super durable: The HALO 2 UPLC core-shell chromatography column has a sieve plate with a pore size of 1.0 μ m (the existing non core-shell sub-2 μ m chromatography column sieve plate has a pore size of 0.5 μ m), which is not easily clogged.

Extremely low back pressure: HALO 2 UPLC core-shell chromatography columns can be used in environments up to 1000 bar (14500 psi), but the resulting back pressure is 20% lower than existing non core-shell sub-2 μ m UPLC chromatography columns.

HALO core-shell chromatography column is a revolutionary product for HPLC, and now the transformation of UPLC has begun

2. High theoretical tray number, low back pressure

3. HALO 2 High Voltage Stability

4. Fast, high-resolution HALO 2

5. Comparative Application Examples of HALO 2 C18 and Conventional 1.7 μ m C18 Chromatography Columns

6. Comparative Application Examples of HALO 2 C18 and Conventional 1.6 μ m C18 Chromatography Columns

7. Example of HALO 2 C18 Separation of Di ****** Hydrazine

Background Introduction of AMT Company

AMT (Advanced Materials Technology) is the father of "core-shell" packed column chromatography technology - Jack from the United States Founded by Dr. Kirkland, he is considered one of the creators of HPLC. The core-shell chromatography packing technology was successfully developed and commercialized in 2006, Jack Dr. Kirkland named it HALO. HALO chromatography column is currently a complete range of core-shell chromatography columns, with product series including:

HALO 2.7 μ m first generation "core-shell" chromatography column (2006) used for ordinary liquid chromatography instruments and achieving approximate UPLC separation

The second-generation "core-shell" chromatography column HALO 5 μ m (2012) is specifically designed for ordinary liquid chromatography instruments and can be directly replaced with conventional 3 μ m chromatography columns without the need for method conversion to improve separation efficiency and speed

2 HALO Bioclass specialized chromatography columns for separation in the field of biology (2013)

HALO 2 chromatography column specially designed for ultra-high pressure liquid chromatography (UPLC) (2014)

Small molecule chromatography column

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HALO 90 To C18

Universal stationary phase suitable for acidic, alkaline, and neutral analysis

Excellent stability when using mobile phases with low to medium pH

A large number of application cases and literature based on this fixed phase can be retrieved

USP classification: L1

Optional particle size: 2 µ m, 2.7 µ m, 5 µ m

New product: Inner diameter 1.5mm product

HALO 90 Å PCS C18

Alkaline compounds have excellent peak shapes and higher sample sizes

Compatible with UHPLC and LCMS systems

Reproducibility and stability between batches

USP classification: L1

Optional particle size: 2.7 µ m

HALO 90 Å AQ-C18

Very suitable for chromatographic analysis of samples with coexisting polar and non-polar molecules

Another chromatographic column with high retention C18 with different selectivity

Can be used for pure water mobile phase

USP classification: L1

Optional particle size: 2 µ m, 2.7 µ m, 5 µ m

Application scope: Suitable for polar insecticides, polar organic acids, and analysis under pure water conditions (e.g. separation of nucleoside based drugs can be completed within 1.2 minutes in 0.1% pure water phase)

HALO 90 To C8

Universal stationary phase suitable for various analytes

Compared with C18, the hydrophobicity of the stationary phase surface is lower, resulting in a shorter retention time

Compared with C18, it is more suitable for the analysis of ion convection mobile phase

USP classification: L7

Optional particle size: 2 µ m, 2.7 µ m, 5 µ m

Application scope: Suitable for the analysis of samples such as cholesterol lowering drugs (statins), flavonoids, and algal oils and fats

HALO 90 YES-CN

Extremely suitable for the analysis of polar compounds

Complementary selectivity with alkyl stationary phase

Can be used for pure water mobile phase

USP classification: L100

Optional particle size: 2 µ m, 2.7 µ m, 5 µ m

Application scope: Can be used for the analysis of samples such as β - lactam antibiotics, nonsteroidal anti-inflammatory drugs, and penicillin MEI inhibitors

HALO 90 Å Phenyl-Hexyl

HALO 90 Å Biphenyl

HALO 90 Å PFP

HALO 90 To RP-Amide

HALO 160 To C30

Hydrophilic interaction chromatography

HALO 90 Å HILIC

HALO 90 Å Penta-HILIC

protein

HALO 1000 Å Diphenyl

HALO 1,000 To C4

HALO 1,000 To ES-C18

HALO 400 To C4

HALO 400 To ES-C18

peptide

HALO 160 YES-C18

HALO 160 AES-CN

HALO 160 Å Phenyl-Hexyl

HALO 160 Å PCS C18

OLIGO

HALO 1,000 OLIGO C18

HALO 120 OLIGO C18

Polysaccharide

HALO 90 To Glycan

PFAS

HALO PFAS PCS Phenyl-Hexyl

Halo core-shell chromatography column