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Free testing and sampling of bovine prealbumin (PA) ELISA kit

NegotiableUpdate on 05/06
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Overview

The free proxy testing of bovine prealbumin (PA) ELISA kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA) to obtain samples. The absorbance (OD value) is measured using an enzyme-linked immunosorbent assay (ELISA) reader at a wavelength of 450nm, and the sample concentration is calculated.

Product Details

Bovine prealbumin (PA) ELISA Kit

:Free testing and sampling of bovine prealbumin (PA) ELISA kit

: XZK-11817

【 Applicable Samples 】: Suitable for liquid samples such as serum, plasma, cell culture supernatant, urine, tissue homogenate supernatant, etc

repetitiveness

The coefficient of variation within and between plates is less than 15%

sensitivity

1.0 mg/L

specificity

Do not cross react with other soluble structural analogues

validity period

6 months

Free testing and sampling of bovine prealbumin (PA) ELISA kit Composition and configuration (please refer to the instruction manual for the detection range)

Product Name

96 hole configuration

48 hole configuration

Storage conditions

Microporous enzyme-linked immunosorbent assay (ELISA) plate

12 holes x 8 strips

12 holes x 4 strips

2-8℃

reference standard

0.3mL * 6 tubes

0.3mL * 6 tubes

2-8℃

diluent

6mL

3mL

2-8℃

Detect antibody HRP

10mL

5mL

2-8℃

20 x washing buffer solution

25mL

15mL

Dilute according to the instructions

Substrate A

6mL

3mL

2-8℃

Substrate B

6mL

3mL

2-8℃

Stop Solution

6mL

3mL

2-8℃

Sealing film

2 sheets

2 sheets

2-8℃

Instruction manual

1 copy

1 copy

2-8℃

self-sealing bag

1 piece

1 piece

2-8℃

In order to help you better solve various problems that may be encountered in experiments, Shanghai Xuanzekang Biotechnology has designed these problem guides, accompanied by the instructions in the kit for your reference:

1. Question: Can ELISA kits be used in batches?

Shanghai Xuanzekang Bioanalysis: It can be used in batches, and the enzyme-linked immunosorbent assay (ELISA) plates in the kit are detachable.

2. Question: How long does the entire ELISA experiment take?

Shanghai Xuanzekang Biological Analysis: From the perspective of experimental methods, the double antibody sandwich method experiment requires about 3.5-4 hours, while the competitive method experiment requires 2-2.5 hours. This time is purely for computer operation, excluding sample collection time and sample processing time.

3. Question: What should I do if the detection limit on the instruction manual is not applicable?

Shanghai Xuanzekang Biological Analysis: The detection limit of our reagent kit is calibrated based on conventional samples. If there are special samples below the detection limit, you can contact our customer service for customized detection limits.

4. Question: Why is it recommended to repeat the sample and standard curve?

Shanghai Xuanzekang Bioanalysis: ① Calculate the average value to ensure the accuracy of the experimental results. ② Prevent jumping holes. ③ Evaluate your own operations. ④ Evaluate the performance of the reagent kit.

5. Question: Does repeated freezing and thawing of the sample affect the ELISA test results?

Shanghai Xuanzekang Bioanalysis: Try to avoid repeated freezing and thawing, and collect and process samples before packaging and freezing. Multiple freeze-thaw cycles can easily cause protein degradation, resulting in a decrease in the level of test values and a relative decrease in trend.

Due to incomplete website information, if you cannot find the product you need in our product center, you can directly leave information about the product you need on the message board or leave a message on the website, and we will contact you as soon as possible.


Free testing and sampling of bovine prealbumin (PA) ELISA kit