Fast counting light staining microplate imaging analyzer, colony counter

Xunshu A100 Light Stained Microplate Imaging AnalyzerIt is designed specifically for porous plates, which can achieve high-resolution full pore large field imaging of different porous plates, count and analyze clones or cell clusters, suitable for: clone formation detection, virus plaque determination Elispot、 Micro Ames test; Large field quantitative analysis of adherent cells (such as Transwell cell invasion and migration experiments, osteogenic induction and detection) can also be used for microdilution drug sensitivity observation, colony counting in gene mutation experiments, growth well imaging recording, nude mouse transplant tumor measurement, and more.

Equipped with a floating dark field, color bright field, and Rheinberg light dye combination illumination mode, combined with high-resolution macro zoom lens and large area global shutter camera for digital imaging, it can capture exquisite images of microcolonies, cell clones, and plaque, making it a powerful tool for scientific literature publication.

Exquisite imaging of microplates

lRich optical path control system

Professional optical path system, capable of achieving single hole suspended dark field illumination and color wide field illuminationcompound eyeBacklight illumination. To adapt to the changes in pore size of different microplatesKnobs andslidercombinedLight field locator,fastRealize changes in the position and angle of the light source,Ensure that the cell clone clusters inside the well receive uniform and bright illumination.

lmultispectralReinberg Lighting Take extraordinary scientific research photos

Xunshu Multi hole Plate with Large Field of ViewReinberglightingTechnology, bycolorfulwide fieldcompound eyeLingtouguang and Huanmu ReversetransmissionThe combination of excitation light is used to reduce glare reflected by the pore wall by selecting different spectra and light intensities, enabling non-destructive light staining of cell clones for easy observation, identification, and counting.

lQuickly meet different field of view requirements

High resolution macro zoom lensQuick viewing capabilityField switching, ensuring6、12、24, 48、Single hole of 96 hole plateOne full screen imaging,Presenting high-resolution and high contrast image quality.It can also be used for quick browsing and observation of porous materials.

lSharp panoramic display of cell population details

The research camera adopts SONY 10 million pixel ultra large area array color CMOS sensor, with double-layer noise reduction and high sensitivity. The global shutter can instantly respond to the rapid movement of the porous plate, presenting the details of cell clone clusters with lossless image quality.

Digital analysis of microplate

lClone formation analysis

Soft agar colony formation assay suitable for detecting tumorigenicity of cells; Plate clone formation experiment for detecting the ability of cell colony formation. Real time preview, shooting, and definition of each well image of cell culture plates, visually displaying single well panoramic imaging of 6, 12, 24, 48, and 96 well plates, automatically counting full well clones (customer defined minimum clone data), and automatically measuring the diameter, area, perimeter, volume, roundness, optical density, etc. of each clone.

lTranswell experiment detects cell migration and invasion ability

lSubcutaneous tumor transplantation experiment in nude mice

The use of nude mouse in vivo inoculation method for cell tumorigenicity examination is a standard test method included in pharmacopoeias of various countriesThe system can automatically measure the length and width of tumors and calculate the volume of transplanted tumors.

lmesenchymal stem cellOsteogenic differentiation induction

Alkaline phosphatase staining、Alizarin red stainingCommonly used to detect the osteogenic differentiation level of stem cells.A100Light Dyeing Imaging SystemEnsure sufficient image resolution even in a large field of viewAutomatic recognition and countingLong spindle shapedQuantitative analysis of color intensity in cells.

lVirus titer analysis-Spot counting

Rheinberg lighting can make crystal violet or neutral red stained cell layers brightly colored, with high image sharpness and easier observation of viral plaques, helping to achieve6、12、24waitorifice plateAccurate counting of erosion spots.

lMicrobacteria Recovery Mutation Test

In the early stages of new drug development, multiple lead compounds need to be synthesized, and the yield of each compound is often very low. Therefore, the Ames test scale needs to be reduced to meet the high-throughput and low-cost research and development requirements. The instrument is suitable for imaging and counting in Mini Ames and Micro Ames experiments.

6-well plate full hole dark field imaging

lGene mutation test

Mouse lymphoma cell gene mutation experimentIn vitro mammalian cellsThe HGPRT gene mutation test isGenotoxicity evaluationThe regular project.

lMicrobial observation and analysis

Suspended dark field transmission illumination allows for rapid observation of the state of micropores (transparent or turbid), precise determination of the critical point of antibacterial activity, and is suitable for the determination of antibacterial concentration using the micro dilution method (broth).

Observation of Microcystis Characteristics

Microscopic analysis

A100The multi energy cell (cluster) imaging analysis software equipped with the light staining microplate imaging analyzer can be used for the analysis of various microscopic images.

lCell fusion degreeevaluation

Cell culture and transfectionGrowth fusion degree during the processIt is an important parameter, and the fast level set multi model algorithm can identify and outline the range of single-layer growing cells, and quickly calculate the percentage of cell coverage area.

Cell Fusion Degree Analysis

lFluorescence cell analysis

The Xunshu multifunctional cell imaging analysis software has rich algorithms such as monochrome classification, multi-color automatic clustering, and multi-color screening, which are suitable for solving monochrome fluorescence analysis such as cell viability determination, cell transfection, and reporter gene expression; Multicolor fluorescence analysis such as immunofluorescence, EDU cell proliferation FISH、 Cell apoptosis.

Main functions and technical indicators

1Structure and Design

ØAll steel aluminum alloy structure, sturdy, exquisite and compact, easy to dissipate heat, good imaging stability

ØCarrier support6. 12、24、48、96 cell culture plate、35mm culture dish、glass slideCellsCounting box、Hemocytometer，Ensure precise positioning of the observed field of view

ØField of view zoom dial: Quickly adjust single hole full screen imaging based on the type of microplate

ØCoarse micro combination focusing: harmonic gear design to prevent focusing from slipping; Fine tuning the dial can quickly achieve precise focusing.

IIOptical path control and imaging

ØDetection mode:Dark field imaging, bright field transmission imaging, Rheinberg light staining imaging

Øcolorfulwide fieldcompound eyebacklightlighting

1)highlightLED compound eye thin bottom transparent base，11Available in colored transmitted light

2)Uniformity of illuminationgreater than93%Ensure uniform illumination at the edges and middle of the micropores to reduce the annular dark circles in the porous plate

ØSuspended dark field illumination

whiteLEDLight source, illumination range 1005500 Lux Color Rendering Index74%

ØRheinberg Light Dyeing Illumination

through11Channel visible excitation light and color wide fieldcompound eyeThe combination of backlighting achieves optical staining of cell clusters, significantly improving observation efficiency and automatic recognition accuracy. Some experiments can eliminate chemical staining steps.

ØLight source controller

1) Invisible suction control panel, dual lighting selection switch, dual channel stepless brightness adjustment

2) Embedded aperture, adaptable to changes in the field of view of different orifice plates, avoids stray light interference, and improves image quality

3) Rotating and sliding combination light field positioning, quickly achieving changes in light source position and angle, meeting the imaging requirements of different orifice plates

ØVariable field of view full hole digital imaging

High resolution macro zoom lens and1.1Inch large array global shuttercamera, achieve6、12、24, 48、96 well plate single hole one-time imaging; Full hole imaging resolution:8µm（96Orifice plate);20µm（6Orifice plate)

IIIPorous plate detection and analysis

ØPre-setselectedOrifice plate function, canLive Preview、Shoot and define cell culture plateseachHole image，Intuitive display6. 12、24、48、Single hole and whole plate images of 96 well plate, facilitating the observation, analysis, and conversion of images within or between different holes

Øachieve6. 12、24、48、One imaging of the entire well of each 96 well cell culture plateTo avoid image imprints and pixel loss caused by image stitching of each hole, which facilitates image observation, archiving, and precise analysis

Ø3D Depth of Field Fusion: Achieving rapid fusion of different focal planes within the same field of view, solving the problem of local blurring caused by clone distribution at different levels, and obtaining panoramic deep and high-definition images

ØAutomatic counting analysis: integrating multi-dimensional seven algorithms, suitable for contour extraction and accurate recognition of clones, cell spheres, organoids, and viral plaques in dark field, bright field, and various coloring situations

ØFull field cloning statistics: cloning total count, cloning total area, cloning coverage, and dividing the statistical results into multiple intervals based on diameter size for display

ØClone morphology measurement: locate monoclonal coordinates, automatically measure the equivalent diameter, perimeter, area, major diameter, minor diameter, volume, roundness, and optical density of each clone

4Microscopic image analysis and processing

ØImplement for6. 12、24、48、96 well cell culture plate、35mm cell culture dish、glass slideCellsCounting boxMid targetAccuracy of imagesrecognizeAnd testing

ØCanassistcomplete① Cell counting, ② Cell cluster detection, ③ Clone formation, ④slightcolony counting、⑤ Cell proliferation, ⑥ cell migration, ⑦ cell apoptosis, ⑧ cell invasion, ⑨ cell toxicity, ⑩ organoid/stem cell spheroidization analysis⑪Traceability analysis of monoclonal antibodies⑫Ames analysis、⑬Cell cycle⑭Bacteriophage plaque/Spot analysis⑮In situ hybridization analysis⑯Gene mutation testing⑰Enzyme linked immunosorbent assay spot analysis ⑱Analysis of biofilm formation, etcvarious, multiple, diverse, a variety ofapplication

ØImage processing: equipped with functions such as image adjustment, adaptive enhancement, sharpening, filtering, edge detection, morphological operations, grayscale conversion, negative phase conversion, etc

ØFeaturing various textual and graphical annotations、Scale bar addition function

ØMultiple accounts can be set up, with different permissions for administrators and experimenters to ensure effective management of experimental data

ØBatch export of images and dataExport data toSave in Excel or PDF format

5Instrument specifications and configuration

ØHost of Light Stained Microplate Imaging Analyzer1 set

ØMicroplate cloning full pore imaging analysis software, multipotent cell (cluster) imaging analysis software