Far infrared carbon dioxide incubator door heating system

Far infrared carbon dioxide incubator door heating systemHH. CHP-01W

Selection of sterilization methods for carbon dioxide incubators

The following are several common sterilization methods and their characteristics for carbon dioxide incubators:
Advantages and disadvantages of sterilization method principle
The use of liquid disinfectants is non corrosive to equipment. Liquid disinfectants are easy to operate and have low costs. The disinfection effect is affected by various factors, such as temperature, pH value, etc
UV disinfection achieves sterilization by destroying the DNA or RNA structure of microorganisms without direct contact, and can cover a large area. It is not effective for areas that cannot be directly illuminated by UV lamps and has certain environmental requirements
Dry heat sterilization uses high temperature (usually 125 ° C) to destroy bacteria and has a good killing effect on bacteria. It takes a long time and may cause damage to certain materials
HEPA filter sterilization can continuously provide a sterile environment by removing microorganisms from the air through high-efficiency air filters. The initial investment is relatively high, and the filter needs to be replaced regularly
High temperature moist heat sterilization uses a high-temperature moist heat environment to denature the DNA or RNA of microorganisms, which has ideal effects on bacteria, fungi, spores, mycoplasma, etc. It requires specialized equipment and relatively complex operation
When choosing a sterilization method, it is necessary to comprehensively consider factors such as the frequency of use of the incubator, the sensitivity of the cultured cells, budget, and specific laboratory conditions.

Far infrared carbon dioxide incubator door heating systemHH. CHP-01W

Precautions for using carbon dioxide incubator

Installation environment: The carbon dioxide incubator should be installed indoors without strong electromagnetic fields and radiation energy, with minimal temperature changes around it.
Power supply usage: Do not turn on the power switch of the carbon dioxide incubator before filling it with water, otherwise it will damage the heating element.
Adding water to the water tank: After the incubator has been running for several months, the water in the tank may decrease due to evaporation, and it is necessary to add water in a timely manner.
Cleaning and disinfection: Add 2 ml of Xinjie'er Mie solution to 1000ml of water, regularly disinfect the air inside the box with alcohol and ultraviolet light, wipe the culture with alcohol into the box, and reduce the growth rate of microorganisms inside the box.
Overtemperature alarm: When the displayed temperature exceeds the set temperature by 1 ℃, the overtemperature alarm indicator light will turn on and emit a sharp alarm sound. At this time, the power should be turned off for 30 minutes. If the power (temperature control) switch is turned on again and still overtemperature, the power should be turned off and maintenance personnel should be notified, and the surrounding environment temperature should be lowered with air conditioning.
Cylinder usage: Before opening the cylinder, be sure to loosen the pressure reducing valve to prevent the gas hose from bursting. When the pressure of the steel cylinder is lower than 0.2MPa, the steel cylinder should be replaced.
Reduce the number of door openings: Try to minimize the time it takes to open the glass door. When opening the door inside the carbon dioxide incubator, the carbon dioxide control should be turned off first.
Precautions for handling: Before handling the carbon dioxide incubator, remove the shelves and humidification tray from the studio to prevent collision and damage to the glass door. When transporting the incubator, do not invert it and do not lift the door to avoid deformation.
The above are the main precautions for using a carbon dioxide incubator. Please note that different carbon dioxide incubators may have some special usage requirements. Please be sure to carefully read and follow the specific guidance and warnings provided by the manufacturer.

General classification of laboratory carbon dioxide incubators:
(1) According to different heating methods, it can be divided into water jacket carbon dioxide incubators and gas jacket carbon dioxide incubators. Generally, laboratories use gas jackets.

(2) According to different disinfection methods, it can be divided into ultraviolet lamp disinfection and high-temperature moist heat disinfection carbon dioxide incubator.

(3) According to the volume score, the commonly used models are around 80L, 160L, 150L, and 200L.

(4) Carbon dioxide control methods include thermal conductivity detection, infrared detection, and automatic proportioning (without sensors)

(5) Display modes: LCD display and digital display

1、 Preparation points before operation
Environmental inspection: It should be placed indoors with good ventilation, no dust, no corrosive gases, and stable temperature. It is recommended to control the ambient temperature at 15-25 ℃. In summer, it is necessary to ensure that the indoor temperature does not exceed 28 ℃, and if necessary, turn on the air conditioning 24 hours a day.
Power supply and installation: Confirm that the power supply voltage matches and is well grounded. Place the instrument on a flat surface to avoid strong electromagnetic fields and radiation environments.
Preheating and Calibration: Preheat and calibrate the CO ₂ concentration (recommended to use pure CO ₂ gas) and temperature after use or long-term disuse to ensure sensor sensitivity.
2、 Daily operation standards
Parameter Settings
Set the temperature, CO ₂ concentration (set to 0 when not in use, otherwise an alarm will be triggered after 15 minutes), and overtemperature alarm values using the Mode key, and press the ENTER key to save.
Adjust the outlet pressure of the pressure reducing valve to 0.06-0.1Mpa to avoid damaging the pipeline due to excessive pressure.
Sample Management
Use sealed containers to avoid overcrowding that affects gas circulation, and regularly inspect and remove contaminated samples.
The incubator door should be tightly closed to prevent gas leakage, and the parameters, date, and time should be recorded after each operation.
Consumables and Environmental Maintenance
The humidification plate needs to be filled with distilled water (tap water cannot be used), the water level should be checked regularly, and the water tank should be replaced annually to prevent corrosion.
When not in use for a long time, the power should be cut off, the humidification disk should be removed, and the box should be cleaned
3、 Cleaning and disinfection requirements
Precautions for frequency operation content
Wipe the inner walls with 70% alcohol or neutral chlorine free disinfectant every week to avoid damaging cells with chlorine containing disinfectants
Regularly (in March) remove the shelf for cleaning and disinfection, clean the condenser of dust, ventilate and dry before starting[
Wipe the working chamber and glass observation window clean before long-term use to ensure there is no residual moisture to prevent mold growth[
4、 Safety and fault handling
Personal protection: Wear gloves, masks, and goggles during operation.
Emergency handling: Familiarize yourself with the alarm system (such as AddWater water water shortage alarm, Replace HEPA filter reminder) and the location of the shutdown button. Replace the CO ₂ cylinder in a timely manner when the pressure is below 1MPa.
Troubleshooting: When the temperature/CO ₂ is abnormal, priority should be given to checking the sensor, air circuit, and heating element. If it cannot be resolved, contact professional maintenance. Disassembly without authorization is prohibited.
5、 Long term maintenance recommendations
Check the status of CO ₂ filters and HEPA filters monthly and replace them according to the manufacturer's recommendations.
During continuous work, check the motor, refrigeration components, and fastening screws every 3 months to ensure equipment stability.
Following the above specifications can effectively ensure the performance of the incubator and provide stability for experiments such as cell culture and stem cell research