DNA Fragment lotion Wrapping and Sorting System

Xdrop is a multifunctional and user-friendly instrument used for preparing live mammalian or microbial cells, organelles, DNA, or other biological materials for high-resolution downstream analysis.

Use ourXdrop kit, Xdrop safely and quickly encapsulates live cells, organelles, or DNA fragments along with assay chemicals in highly stable double emulsion droplets with a skin volume. These droplets are stable through pipetting, vortexing, incubation, flow cytometry, sorting, and even long-term storage. More importantly, cells can be recovered from droplets for expansion.

Together with appropriate analytical techniques,The Xdrop application includes:

• Human immune cellsNK cells

• Enzyme secretion of microbial cells

• Mammalian cells secrete cytokines

• Validate gene editing, including evaluating unexpected on target and off target rearrangements

with the help ofXdrop， Researchers can more easily gain a deeper understanding of the cellular functions and genomics of humans, animals, plants, and microorganisms. Xdrop can also generate single lotion droplets for other workflows, including packaging DNA for unbiased genome-wide amplification. We provide the complete set of reagents, kits, and accessories required for Xdrop. In addition, user training is part of our standard Xdrop installation service.

advantage

• Xdrop can wrap living mammalian or microbial cells in highly stable double lotion drops for function determination, incubation, phenotype analysis and screening.

• Xdrop helps answer key questions in engineering cell and gene therapy research.

• Xdrop supports molecular engineering workflows, including enzyme evolution and pathway engineering.

• Xdrop has a user-friendly interface and a small footprint, allowing every molecular biology laboratory to utilize the * capabilities of microfluidics.

Double layer emulsifying reagent kit

All reagents are included in the kit, which can encapsulate millions of cells or molecules per operation.

Application example:

1Accurate amplification of the entire genome

In fact,99% of the target genome is covered by sequencing reads from the library more than once.

useXdrop ™ The dMDA technology amplifies genes with high fidelity and accuracy, even when the input volume is as low as 1 pg.

2. Genetic phase determinationPhasing

The CYP2D6 * 7 allele contains one single nucleotide polymorphism（SNP）， CYP2D6 * 25A contains 22 SNPs.

Xdrop technology can accurately detect all 23 variants, including Phasing (seedownImage).

Xdrop ™ Provide support for applications through simple design and indirect sequence capture of long DNA fragments (~100 kb). Combined with long read sequencing, Xdrop ™ Overcoming the difficulty of gene phasing in a single genetically heterogeneous sample.

Enrichment with high fidelity can detect one or more allelesSNP， And enable self-assembly from scratch to solve structural changes.

3. Monitoring complex structural changes in genes

A detailed analysis of readings crossing breakpoints shows thatThere are multiple HPV18 integration sites in the region on chromosome 8. The PacBio, Oxford Nanopore, and Illumina datasets support identified integration sites.

Xdrop ® Technology is a new targeted DNA enrichment method, which has the advantage of being able to select, enrich, and sequence natural DNA fragments. This allows for enrichment of large genomic regions (approximately 100kb), including unknown areas. Using Xdrop ® The long fragments generated by the method are not only suitable for long read sequencing, but can also provide valuable contextual information for short read sequencing.

4. For unknown areas of interest（ROI sequencing

PCR and probe based targeted DNA enrichment methods require intensive design optimization andComplete area of interest（ROI knowledge. These traditional methods are used to characterize complexityThe genomic environment (such as repetitive regions, structural variations, and abundance)GC's zoneIt also failed in terms of domains, as well as unknown and rearranged regions. To overcome these limitations,Samplix has developed a new microfluidic method based on indirect sequence captureObtainedXdrop ® Enrich workflow.

Xdrop ® It is possible to enrich the length of a single primer pair that needs to be designed on the detection sequence（~The 100 kb target DNA region corresponds to a small portion or flanking area of the ROI. This amplicon is specifically designed for detecting, selecting, and enriching full-length ROIs, ultimately for capture and sequencing.

useXdrop ® The ability of technology to address these challenging areas has opened the door to solving many other challenging areas, which may represent a significant proportion of the baseBecause of the group. Translate into EnglishXdrop ® The combination of enrichment and long read and short read sequencing technologies can provide the required high resolution to quickly and economically solve complex genomic scenarios, bypassing whole genome sequencing.

5. Identification and confirmationCRISPR gene editing operation

Through simple design and lengthyIndirect sequence capture of DNA fragments, Xdrop helps identify transgenic insertion sites that are often difficult to find and costly.

The random integration in the construct and the presence of host genome sequences make the identification of integration sites complex.Xdrop makes validating genome engineering simple and clear.