Circular dichroism analyzer

NegotiableUpdate on 12/29

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Overview

The manufacturer of circular dichroism analyzer introduces that circular dichroism analyzer works based on the phenomenon of circular dichroism. When linearly polarized light passes through a sample with chiral molecules, due to the different symmetries of the molecules, the two chiral molecules will cause the polarization direction to rotate, which is called optical rotation property. If an elliptically polarized light is introduced into polarized light, the optical rotation of the two chiral molecules towards it will also be different. Therefore, the properties of chiral molecules present in the sample can be determined by measuring the optical rotation of the sample towards elliptically polarized light.

Product Details

Circular dichroism analyzer is a spectroscopic instrument used for analyzing chemical substances. It can measure the effect of sample rotation on elliptically polarized light, thereby obtaining information about the structure and chemical properties of the sample.

1、 Principle

The circular dichroism analyzer operates based on the phenomenon of circular dichroism. When linearly polarized light passes through a sample with chiral molecules, due to the different symmetries of the molecules, the two chiral molecules will cause the polarization direction to rotate, which is called optical rotation property. If an elliptically polarized light is introduced into polarized light, the optical rotation of the two chiral molecules towards it will also be different. Therefore, the properties of chiral molecules present in the sample can be determined by measuring the optical rotation of the sample towards elliptically polarized light.

In practical applications, the circular dichroism instrument uses a device consisting of a rotating plate and an ellipsoidal polarizer to introduce elliptically polarized light. The sample is placed in the optical path and passes through the rotating plate and polar plate before finally reaching the detector. The detector can measure the degree of light absorption of the sample and calculate the degree of rotation. Based on the magnitude and sign of the rotation degree, information such as the type and quantity of chiral molecules present in the sample can be determined.

2、 Construction

The circular dichroism analyzer mainly consists of a light source, monochromator, polarizer, standard sample, sample chamber, detector, and other parts.

Light source: Provides a variable wavelength light source, usually choosing Xe or Hg lamp light sources.

Monochromator: Used to select monochromatic light within the appropriate wavelength range to avoid unnecessary interference.

Polarizer: used to generate linearly polarized light and elliptically polarized light. Usually, Bell mirrors or electric modulators are used to introduce elliptically polarized light.

Standard sample: used for calibrating instruments and conducting quantitative analysis.

Sample room: a container for storing the sample to be tested. Usually quartz or glass materials are used to avoid interference with the sample.

Detector: used to measure the degree of light absorption of the sample and calculate the degree of rotation. Common detectors include polarizers, photodiodes, etc.

3、 Application

Circular dichroism is widely used in fields such as chemistry, biology, and medicine. It can be used to study the structure and chemical properties of organic compounds, proteins, nucleic acids, and other samples, as well as drug metabolism and reaction kinetics processes. Specific applications include:

Determine the chiral properties of organic molecules.

Study the secondary structure and fluorescence properties of macromolecules such as proteins and nucleic acids.

Study the mechanism of action and efficacy evaluation of drugs on living organisms.

Analyze the nutritional components, additives, pollutants, etc. in food.

In short, circular dichroism is a very important chemical analysis instrument with wide applications in chemistry, biology, medicine and other fields.