Cherry blossom embedding agent

Cherry blossom embedding agentIntroduction:

Japanese Sakura SAKURA Cryo Embedding Agent OCT Cryo Slice Embedding Agent 4583 118ml/bottle

OCT embedding agent (SAKURA product) embedding process:

1. Remove the tissue block from the sucrose solution and immerse it in a 1:1 volume ratio mixture of 20g L-1 sucrose and OCT embedding agent at room temperature for 2 hours

2. Transfer to OCT embedding agent and soak at room temperature for 4 hours. Replace with a new OCT embedding agent and soak at room temperature for 6 hours

3. Place the specimen on a tray and slice it using a constant cold box slicer (usually -22 ℃) with a thickness of 10 μ m. Attach it to a pre treated glass slide and store it in a -20 ℃ freezer.

Cherry blossom embedding agentApplication:

OCT frozen section embedding agent is a water-soluble mixture of polyethylene glycol and polyvinyl alcohol, which is widely used in immunohistochemistry laboratories. Its purpose is to support tissues during frozen section to increase tissue continuity, reduce wrinkles and fragmentation. Also, as the OCT mixture is water-soluble, it is soluble in water during the bleaching process, so background staining will not be added in future staining.

In addition, reactions in skin diseases account for a large proportion of their etiology and pathogenesis, so it is important to display antigens or antibodies in the diagnosis of skin biopsy specimens. Generally, frozen section direct immunofluorescence method is used to observe the fluorescence antibody staining results on the section specimens as the identification and localization of antigens

Using OCT mixture (optim um cutting temperature compound) to pre infiltrate tissues, and then performing constant cold box sectioning to improve the quality of sectioning.