- Phone
-
Address
J, 1st Floor, 185 Moyu Road, Jiading District, Shanghai
Product Categories
Shanghai Youbai Biotechnology Co., Ltd
Beijing Shengxia Protein O-Glycoproteinase
NegotiableUpdate on 05/06
- Model
- Nature of the Manufacturer
- Producers
- Product Category
- Place of Origin
Overview
Beijing Shengxia protein O-glycoprotein has broad specificity and is an O-glycoprotein cloned from Pseudomonas aeruginosa. This enzyme can recognize mucin type O-glycans (including branching and sialylation structures) in glycoproteins or glycopeptide chains, and cleave peptide bonds into glycosylated serine or threonine residues (cleavage at the N-terminus).
Product Details
Common types of O-glycoproteases
(1) O-Glycosidase
source: Usually comes fromEnterococcus faecalisorStreptococcus pneumoniae.
specificity：
cuttingCore 1(Gal β 1-3GalNAc) andCore 3(GlcNAc β 1-3GalNAc) O-sugar chain, but cannot cleave sialylated O-sugar chain (sialic acid needs to be removed first).
Not applicable to other core structures (such as core 2/4).
applicationSimplified O-chain analysis, commonly used for mass spectrometry or HPLC pretreatment.
(2) Non-specific O-chain releasing enzyme
chemical method：
β - elimination reaction (alkaline conditions)If treated with NaOH/NaBH ₄, the O-sugar chain is released and its end is reduced, but it will damage the peptide chain.
Enzymatic combination：
Sialidase+O-glucosidaseThe sialic acid residues need to be removed first, and then the core structure needs to be enzymatically cleaved.
Our company independently develops-Beijing Shengxia Protein O-Glycoproteinase（IMPa）， Also known as immunomodulatory protease (IMPa), it is a type of enzyme derived from Pseudomonas aeruginosa（Pseudomonas aeruginosa）， O-glycosidase with broad-spectrum specificity. It recognizes mucin type O-linked sugar chains in glycoproteins or glycopeptides, including sugar chains with branched and sialylated structures, and cleaves the N-terminal peptide bond adjacent to glycosylated serine or threonine residues. It can be used in the experimental process of glycoproteomics to determine the O-glycosylation site map and perform structural analysis on the O-sugar chains present at each glycosylation site. O-glycoprotein contains 6 × His tags, which can be easily removed from the reaction system using nickel affinity resin.
Product Features
Used for O-glycosylation site determination and O-sugar structure determination
Effectively cleaves glycoproteins (regardless of sialylation) without the need for neuraminidase treatment
For recombinant enzymes, without contamination from other proteases
Can be used under both denaturing and non denaturing conditions
Free of glycerol, easy to achieve excellent results in HPLC and MS analysis
Provided in solution form; Excellent activity and stability for up to 24 months
Beijing Shengxia Protein O-GlycoproteinaseContaining 6 × His tags, it can be easily removed from the reaction system using nickel affinity resin
