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Beijing Saitaike Biotechnology Co., Ltd
BD LSRFortessa flow cytometer
NegotiableUpdate on 12/23
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Overview
The BD LSRFortessa flow cytometer is the preferred flow cytometer with powerful functionality, performance, and consistency. The design of BD LSRFortessa is reasonably priced and scalable, with flexibility to meet the growing demand for multi-color flow cytometry detection.
Product Details
BD LSRFortessa flow cytometer
BD LSRFortessa ™ Cell analyzer is the preferred choice for flow cytometry, with powerful functionality, performance, and consistency. The design of BD LSRFortessa is reasonably priced and scalable, with flexibility to meet the growing demand for multi-color flow cytometry detection.
feature
The flexibility of small spaces
BD LSRFortessa ™ The system is a reasonably priced choice that meets the needs of most flow cytometers, and can be configured with up to 7 lasers * - blue, red, purple, UV, and yellow green. The flexibility of laser wavelength allows for the optimization of analytical designs using new fluorescent dyes and substrates. This instrument can use a defined set of optical filters to simultaneously meet the detection requirements of up to 18 colors, which can meet or exceed most of today's measurement requirements. BD LSRFortessa is a validated innovative platform that enables multi-color analysis.
BD LSRFortessa will test the proven BD ™ The functions of the LSR platform are placed in a compact space. It can be easily installed on desktop computers to save costs.
For laboratories with limited space or high space costs, this space efficiency is even more important. In addition to reducing size, design enhancements also make it easier to access bandpass filters and reflectors, simplifying the experimental setup
Fluid system
Outstanding performance
The BD LSRFortessa product line integrates many innovations. The flow cytometer system is the heart of the flow cytometer, and has a real fixed ratio flow cell. The flow cell gel is coupled to the acquisition optics to detect signals
Fluid system
Fluid systems are pressure driven. Fluid dynamic focusing forces the sample cell to pass through the colorimetric cup flow cell, where it is subjected to. The flow cell is aligned with the laser, and the gel is coupled to the acquisition optics. This design ensures precise focusing of the laser on the sample stream and can collect the maximum amount of emitted light, thereby improving sensitivity in multi-color applications. Fixed alignment can also shorten the start-up time, improve the reproducibility of experiments, and achieve automated daily quality control.
The sheath container (8 L) and waste container (10 L) are located outside the cell analyzer and placed on the floor for easy access.
Optional BD FACSFlow ™ The supply system jet cart can increase capacity and be easy to use, while maintaining stable jet pressure. It includes an automated sheath and waste control system, combined with two 20 liter containers (Cubiteiners) ®） To reduce daily maintenance.
The fluid sensor maintains a constant pressure, while the fluid monitoring system issues a warning when the sheath fluid is insufficient, emptied, or the waste liquid container is full.
Cell flow cell: the quartz cell flow cell gel is coupled to the collection optics. This design helps ensure that the laser is precisely focused on the sample stream to generate the maximum signal and ensure the collection of the maximum amount of emitted light.
Fluid dynamics focusing of sample core through flow cell
optics
Maximum signal, minimum crosstalk
Collecting optical devices is another design innovation. They are arranged in octagonal and triangular optical paths, and their novel design effectively enables signal detection while improving sensitivity and resolution. This enables researchers to identify cells, especially dim and rare cell populations, optimize multi-color analysis and panel design to achieve excellent results.
optical system
The optical system consists of a laser excitation optical system that irradiates cells in the sample, and a collection optical system that guides light scattering and fluorescence signals to the detector through a spectral filter. The innovative design of excitation optics and collection optics in the BD LSRFortessa system can reduce excitation losses and optimize collection efficiency, thereby improving sensitivity and resolution.
Excitation optics
The excitation optical device consists of multiple fixed wavelength lasers, beam shaping optical devices, and individual pinholes that cause spatially separated beam spots.
The last lens focuses the laser into the gel coupled cuvette flow cell. Due to the fixed optical path and sample core flow, the alignment between daily experiments is constant.
Collecting Optics
The light from the gel coupled cuvette is transmitted to the detector array through optical fiber. Collecting optical devices set in octagonal and triangular optical paths can improve the signal detection capability generated by each laser irradiation beam point. The bandpass filter in front of each PMT allows for spectral selection of the collected wavelengths. Importantly, this arrangement allows for easy modification of filters and mirrors within the optical array, and maximum signal strength can be achieved without the need for additional alignment.
Transmission pathways in octagons
Emission spectra of commonly used fluorescent dyes
This design is based on the principle that light reflection is more effective than light transmission. The emitted light propagates through reflection to each PMT detector and only passes through two pieces of glass to reach the detector. Therefore, more colors can be detected with minimal light loss.
Control and Analysis
Higher consistency
BD FACSDiva ™ The software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. This software is used in BD FACS ™ Instrument series (including BD FACSCanto) ™ Cell analyzer and BD FACSARia ™ Universal in the system. This provides researchers with greater application flexibility, allowing them to easily transfer measurements from one platform to another.
Cell analyzer setup and tracking
The Cytometer Setting and Tracking (CS&T) function of BD FACSDiva software can establish baseline settings and adjust for instrument variability. This software reduces operator errors and ensures consistency of results by setting signal time delays across multiple laser beams and optimizing PMT voltage. You can also set application specific settings to quickly set up and perform routine experiments in a more consistent manner. The quality control (QC) tracking function in this software can measure instrument settings and report performance. Levey Jennings diagrams can help users understand instrument performance and identify maintenance issues.
Collection and Analysis
The BD FACSDiva software enables researchers to preview and record data from multiple samples through automated collection processes. The collection template, user-defined experimental layout, and simple compensation program are also managed by the software to further facilitate collection.
The BD FACSDiva worksheet displays a well-defined subset of T cell populations obtained using a specially configured BD LSRFortessa analyzer, which is equipped with the following lasers: 60 mW 355 nm ultraviolet, 100 mW 450 nm violet, 100 mW 488 nm blue, and 40 MW 640 nm red.
For analysis purposes, the software includes powerful features such as layered capture gates, various drawing formats, and batch analysis. You can analyze recorded data by creating graphs, gates, population hierarchies, and statistical views on the BD FACSDiva global worksheet. After saving the global worksheet, it can be used to analyze multiple sample tubes in the experiment, saving time. Many other productivity advantages include user-defined batch analysis and automated features, such as door resizing, pausing between data files, exporting statistical information, and printing before continuing to the next data file.
option
BD ™ High throughput sampler options
To improve the experimental workflow, optional BD ™ The high-throughput sampler (HTS) provides fast, fully automated sample collection from 96 and 384 well microtiter plates. In high-throughput mode, the HTS option can accelerate the speed of passing through a 96 well plate in less than 15 minutes, with less than 0.5% residual sample from one well to another. In research applications, low residue isnecessaryTo ensure sample purity and data integrity.
By synchronizing two high-precision pumps for sample mixing, sample injection, and probe cleaning, fast collection speed can be achieved. Standard flux mode can be chosen to collect larger sample volumes.
BD high-throughput sampler (HTS)
Special order products
The BD Special Order Research Program allows customers to configure BD flow cytometers to meet precise research and measurement needs. This program is tailored to meet the specific needs of cutting-edge biomedical research, providing a wide range of options to help researchers create customized instruments based on their own needs.
sample data
Analysis of regulatory T cells
Regulatory T cells (Tregs) play a crucial role in maintaining immune regulation and are present in small quantities in normal peripheral blood (5-10% of CD4+T cells or 1-2% of total lymphocytes). Forkhead box P3 (FoxP3) is considered a decisive marker for this rare cell population. It is known that Treg can express CD25. The CD4+CD25+(high) gating strategy displayed here is used to identify Treg populations. These figures represent data collected from BD LSRFortessa using an 8-color panel that applies this gating strategy to identify Fox P3 positive cells.
Human blood analysis
The data is displayed on a 10 color panel running on a 3 laser BD LSRFortessa with blue, red, and purple configurations. Using lysed whole blood from normal donors (BD Pharm Lyse) ™ Using lysis buffer, the panel is used to define T cell subpopulations. BD Horizon ™ The use of V450 and BD Horizon V500 dyes allows brighter fluorescent dyes to be used to express dimmer antigens, while the use of 10 colors can expand the amount of information obtained from a single test tube.
BD LSRFortessa flow cytometer
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