BCA protein quantification kit

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

The BCA principle is that divalent copper ions can be reduced by proteins to monovalent copper ions under alkaline conditions. The interaction between monovalent copper ions and BCA Solution A (containing BCA) produces a sensitive color reaction. Two molecules of BCA chelate a copper ion to form a purple reaction complex. The water-soluble complex exhibits strong absorbance at 562nm, and there is a good linear relationship between absorbance and protein concentration over a wide range. Therefore, protein concentration can be calculated based on absorbance values.

Product Details

Product Features:

The steps are simple and can be completed within 45 minutes, which is 4 times faster and more convenient than the classic Lowry method.
High sensitivity, with a detection concentration limit of 25 μ g/ml and a minimum detection protein amount of 0.5 μ g, and a sample volume of 1-20 μ l.
The BCA method for determining protein concentration is not affected by most chemical substances such as detergents in the sample, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween 20, 60, 80 in the sample.
There is a good linear relationship within the concentration range of 20-2000 μ g/ml.
The coefficient of variation for detecting different protein molecules is much lower than that for protein quantification using the Coomassie Brilliant Blue method.