Bio Excellence International Tech Co., Ltd., abbreviated as Bio Excellence, is an international leading life science equipment, consumables, and reagent sales and technical service enterprise established in Beijing in 2010.

Bioexcellence refers to the collaboration of doctoral students from the global life science community to deliver professional and effective life science products and services to manufacturers and users. It introduces international cutting-edge products and technologies in life sciences, including biomechanics, 3D bioprinting, regenerative medicine, biomedical engineering, microfluidics&tissue organ chips, and cellular microenvironments, to China, providing Chinese researchers with professional and effective one-stop solutions for instruments, equipment, consumables, reagents, and scientific research technology services.

New Introduction of Shilian BoyanTIRF Total Internal Reflection Fluorescence Microscope Turnkey Workstation

TIRF Total Internal Reflection Fluorescence Microscope Turnkey Workstation

——Complete types: objective, prism, and optical guided total internal reflection microscopes, with flexible and economical combinations

The TIRF total internal reflection fluorescence microscope workstation can flexibly integrate the advantages of prism based, optical guided, and objective based total internal reflection fluorescence microscopes, infinite objective 20x, 40x, 60x, or 100x, low light CMOS cameras, and multi-color fiber coupled illumination technology.
● Multiple configuration options: Prism, optical guide, and objective lens optional and flexible combination
● There are complete workstation style and existing microscope upgrade accessory style
● Includes open perfusion chamber and closed flow chamber, optional electrochemical and dielectrophoresis control devices, etc
● Support TIRF flow system perfusion
● Support on-demand customization

The optical schemes based on prisms, optical guides, and objective lenses are the three mainstream geometric configurations used for TIRF microscopy analyzers. The advantages and limitations of each configuration are as follows:

1) The prism based scheme provides excellent signal-to-noise ratio, however, it is difficult to implement in the scheme of using an open perfusion chamber on an inverted microscope.
2) The geometric structure based on optical waveguide has excellent flexibility and signal-to-noise ratio, which can be used with open perfusion chambers on inverted microscopes, and is compatible with dry, water immersed, and oil immersed objectives. However, it requires greater optical power to obtain the same intensity of evanescent waves.
3) Emission fluorescence ratio collected based on eyepiece schemeDrunken BigHowever, the quality of the TIRF effect is greatly reduced because the excitation light and the emission light share the same optical channel, resulting in a large amount (~15%) of stray light also exciting most of the samples, thus deteriorating the TIRF effect generated by the eyepiece TIRF.

thatTIRF Total Internal Reflection Fluorescence Microscope Turnkey WorkstationFully flexible integrationprismtypeLight guidetypeThe advantages of the three types of TIRF with objective lenses,There are multiple flexible configurations to choose from, including open perfusion chambers and closed flow-through chambers, optional electrochemical and dielectrophoresis controls. fully reflectsThe high sensitivity and high resolution advantages of total internal reflection fluorescence microscopy.

We also support providing additional accessories for prism, light guide, and objective TIRF unit fluorescence microscopes separately, upgrading your existing microscope to a total internal reflection microscopy analysis system.

Flexible combination schemes can achieve realistic resultsThe signal-to-noise ratio, use of an open perfusion chamber on an inverted microscope, excellent flexibility in use, and outstanding signal-to-noise ratio canCompatible with dry, water immersed, and oil immersed objective lensesCollected emission fluorescence ratioDrunken Big， The quality of TIRF effect is not discounted and can be adjusted according toChoose the configuration according to actual needs.

Better cost-effectiveness than Nikon, Olympus, Zeiss, and Leica brands:

For various reasons, Nikon, Olympus, Zeiss, and Leica have been sayingA total internal reflection fluorescence microscope relying solely on expensive high NA targets. A typical objective based total internal reflection fluorescence microscope costs approximately $80000 or more. Ironically, in terms of total internal reflection fluorescence microscopy, high price does not necessarily mean high quality. The significant deviation between evanescent waves and theoretically predicted exponential decay has been well documented in the literature.People should take into account the fact that researchers are unwilling to disclose negative results about failed attempts. In many cases, the intensity of stray light is comparable to that of evanescent waves; Objective type total internal reflection fluorescence microscope users often deal with evanescent waves contaminated by more than 50% stray light.

We provide a total internal reflection fluorescence microscope workstation in the United States that can flexibly combine prism type total internal reflection fluorescence microscopes, optical guided total internal reflection fluorescence microscopes, and objective type total internal reflection fluorescence microscopes according to your needs, effectively solving this problem.

Widely used:

We offer American Total Internal Reflection Fluorescence Microscopy as an analytical tool in various fields of life sciences, including single-molecule detection, super-resolution microscopy, cell biology, real-time microarrays for molecular diagnostics, development of biological assay methods, nanotechnology, and drug screening.

Lipid raft dynamics

• Observation of cell surface images: cell membrane surface structure, cell surface contact, membrane surface dynamics/protein localization.

• Single molecule observation and manipulation: Myosin, actin, and Cy3 labeled ATP.

• ● Cell membrane surface movement: such as vesicular swallowing, vesicular spitting, and extracellular secretion.

• Observation of calcium spark phenomenon on cell membrane and monitoring of ion channels.

• Molecular motor research: Rotating motors, cytoskeletal proteins, polymers, G proteins, cyclic proteins, nucleotide motors.

• Single molecule detection, including smFRET

• ● Super resolution microscopic copy

• ● Cell membrane research

• Molecular Diagnostics

• Analysis of Interactions between Biomolecules

• Real time dynamics monitoring of interactions between biomolecules

• Real time TIRF microarray

• Combined arrays of deoxyribonucleic acid, ribonucleic acid, proteins, and metabolites

• Research on protein-protein and protein-DNA interactions

• Research on Surface Supporting Membrane

• ● Study on the dynamics of lipid rafts

• Nanoengineering

• Development of biometric assays, etc

In addition to the biological field, it also has good applications in the observation of chemical molecular structures in the chemical field.

Portable TIRF biosensor:

It is an autonomous (small shoe box size) device used for instant molecular diagnosis. This portable TIRF biosensor is capable of performing highly multiplex analysis - simultaneously detecting from several to hundreds of DNA, RNA, protein, and metabolite markers. The response speed ranges from a few seconds to a few minutes.

Portable TIRF biosensorNo or minimal sample preparation is required. Whole blood, fine needle aspiration biopsy lysate or tissue homogenate samples can be analyzed with minimal preparation procedures.thatPortable TIRF biosensorMeasure the real-time dynamics of microarray response, construct sensing maps, and obtain k-on and k-off rate constants. The sensor is equipped with internal standards, positive and negative controls printed on the surface of the TIRF sensor chip.thatPortable TIRF biosensorUse silica, glass, and plastic microscope slides (1 inch x 3 inches x 1 millimeter) as TIRF optical guides.Portable TIRF biosensorIt also supports electrochemiluminescence (ECL), bioluminescence, bead based and solution phase fluorescence detection methods. The sensor is very suitable for real-time dynamic analysis of bioassay packaged with surface fixed gel.Portable TIRF biosensorCustom configurations include multi-color lighting fixtures, temperature and electrochemical control.

Shilian Boyan has more than 10 years of experience in international cutting-edge scientific research instrument agency sales. It has successfully established good and trustworthy long-term cooperative relationships with nearly 50 scientific research product manufacturers in the international life science field, as well as more than 1300 Chinese tertiary hospitals, more than 2100 scientific research institutes, more than 600 universities, and more than 400 inspection and quarantine units. By continuously introducing international cutting-edge life science scientific research products, professional and large-scale marketing channels, efficient and safe international intermodal transportation, fast customs clearance and declaration, and professional and humanized pre-sales and after-sales service models, Shilian Boyan has achieved the docking and implementation of international manufacturers and Chinese scientific research users, realizing the sharing of partner resources and common development It is an open and enthusiastic enterprise unit that warmly welcomes relevant personnel from domestic and foreign units to negotiate, cooperate, and seek common development.
The customer scope of Shilian Boyan:
Research institutes, biomedical research universities, hospital basic research units, etc.
The brands represented by Shilian Boyan Company include:
1) Long term stable supply of goods in the past 10 years
2) Mainly focusing on biomechanics, cellular mechanics, cellular biomolecules, biomedical tissue engineering, and biomaterials, while also considering other related product lines
3) Provide professional product training and sales training
4) Good technical support
5) Verification of old customers who have already completed transactions
6) Newly added sources of goods every year.