Biomolecular Structure Analysis System

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

RedShiftBio AQS3pro is a new analytical technique that can directly detect the biophysical structure of proteins. It provides secondary structural information of the surface in an automated analysis, involving five key measurements - protein aggregation, quantification, stability, similarity, structure - replacing the requirement of running samples on multiple instruments. AQS3pro directly supports protein characterization throughout the entire biopharmaceutical development process by enhancing biological analysis, and provides new insights for protein based drug development.

Product Details

AQS3 Pro Biomolecular Structure Change Detection and Analysis System, Microfluidic Modulation SpectroscopyAutomatic Analysis System for Biomolecular StructureProtein Preparation and Identification Platform, Protein Preparation and Identification System

RedShiftBio AQS3proUse microfluidic modulation spectroscopy (MMS) to measure protein structure, stability, aggregation, concentration, and protein structure similarity.

Measuring principle	Microfluidic Modulation Spectroscopy (MMS)
application	Protein conformation
Sample Type	Proteins and peptides
concentration range	0.1 to>200 milligrams per milliliter
automation	Customized 24 hole and 96 hole plate formats
Optical detector	Quantum cascade laser (1590-1710 cm-1)

Product Overview

RedShiftBio AQS3proIt is a new analytical technique that can directly detect the biophysical structure of proteins.It provides secondary structural information of the surface in an automated analysis, involving five key measurements of protein aggregation, quantification, stability, similarity, and structure, replacing the requirement of running samples on multiple instruments.AQS3pro directly supports protein characterization throughout the entire biopharmaceutical development process by enhancing biological analysis, and provides new insights for protein based drug development.

AQS3pro allows users to "see changes in protein secondary structure" within a wide concentration range from 0.1mg/mL to over 200 mg/mL and in the presence of excipients, across the entire drug development spectrum.With minimal preparation, samples can be measured without dilution, and reliable data representing drug performance can be generated.

The microfluidic modulation spectroscopy (MMS) technology, which has been developed, combines mid infrared laser spectroscopy with microfluidic and built-in real-time reference signal processing to provide high-sensitivity measurements without buffering interference.The tunable laser in the secondary structure analysis tool detects protein solutions through microfluidic cells.Microfluidic cell fluid rapidly alternates between sample flow and reference (buffer) flow, continuously and automatically performing background subtraction, thereby significantly improving measurement accuracy, precision, and signal-to-noise ratio.

AQS3delta analysis software can automatically process result data to elucidate protein behavior and simplify the format presentation of information flow

Advantages of AQS3 Pro Biomolecular Structure Change Detection and Analysis System:

Understanding protein behavior through 5 key measurements - aggregation, quantification, stability, structure, and similarity

Wide concentration range, from 0.1 to over 200mg/mL, can characterize biological therapeutic drugs

Automated multi sample analysis for unmanned operation

Compatible with almost all buffers and additives;No more labeling, adulteration, or dialysis

High sensitivity and sensitivity, capable of detecting small changes in protein secondary structures, and possessing excellent repeatability and reproducibility
Analysis software can easily convert data into insights

application field

gatherThis is a common indicator of protein instability, which may make therapeutic products unsuitable for marketing.MMS is one of the technologies that can directly monitor the formation of aggregates, as it can measure the intermolecular β - folding structure.

Protein concentration quantificationThe structure and behavior of proteins in a solution may be a function of their concentration, so the ability to accurately quantify protein concentration can better compare different proteins and formulations.The high resolution (<0.001 cm-1) and low stray light sensitivity of MMS increase the measured linear concentration range by more than 2 orders of magnitude.The small measurable concentration of this technology is less than 10 μ g/mL, with an upper limit greater than 200 mg/mL. Compared with traditional absorbance based measurements, it has significant improvements.

Protein higher-order structures (HOS) -During the manufacturing process, biopharmaceutical proteins may undergo conformational changes, altering their secondary structure and thus changing their function.MMS can detect these changes in formulated buffer solutions and target concentrations with high sensitivity without dilution or deuteration.MMS provides detailed information on which structural motifs in protein molecules are undergoing changes to help guide the development of more stable protein molecules and formulations.

Protein stabilityIt is crucial to measure the stability of proteins to heat or chemical exposure during manufacturing and storage processes.Structural changes may lead to decreased efficacy, product degradation, and increased impurities and aggregates, which may be harmful.MMS technology can accurately evaluate the stability of proteins throughout the entire formulation, development, and manufacturing process, thereby reducing risks and providing control strategies for each critical quality attribute (CQA).

生物相似性In addition to functional comparison, measuring and analyzing the structural similarity between proteins is an effective method for demonstrating bioequivalence.MMS measures the secondary structure of proteins, revealing small conformational differences between different proteins and providing information about where these differences occur.These features make MMS the * tool for biosimilar drug analysis and development.