isothermal calorimeter

calScreener^TMIt is a cell analysis system that does not require labeling for real-time detection of live cell heat flow.calScreener^TMBased on mature isothermal microcalorimetry technology, it is suitable for scientists to study the needs of bacteria and mammalian cells. Isothermal calorimetry is a label free technique that not only makes the experiment cost-effective, but also provides great flexibility in sample morphology. This technology can be used for traditional two-dimensional cell culture and novel three-dimensional culture of mammalian cells, bacteria, fungi, parasites, as well as tissue samples.calScreener^TMSuitable for all cell types and cell medium compositions, with a special and wide range of applications.

calScreener^TMIt can provide researchers with long-term real-time data and provide new insights for the study of biological processes, which traditional endpoint analysis methods cannot achieve. All living cells generate heat through the chemical and physical processes of life; By monitoring the changes in heat flux over time (0/s, W), a wealth of information about biological systems can be obtained.isothermal calorimeterAnalysis based on calorimetry is the true measurement of phenotypic responses caused by external conditions or stimuli. All changes in stimulation or environment will be reflected in the metabolic state of cells, which can be directly determined bycalScreener^TMmonitor.

This type of phenotype based analysis monitors the net effects of all cellular processes, including synthetic and catabolic metabolism, providing researchers with a tool to monitor the metabolic status of cells in complex biological reactions. Traditional analytical methods, such as combined analysis, metabolite monitoring, or expression level measurement, can only provide a simple portrayal of specific events or states of cellular life.calScreener^TMProvided a new method for real-time tracking of metabolic processes.

calScreener^TMisothermal calorimetercanUsed for various types of cell analysis. Growth, apoptosis, necrosis, thermogenesis, and differentiation can all be monitored. The application areas include component screening, lead optimization, toxicology, antibiotic development, parasitology, environmental monitoring of water and soil samples, etc.

Continuous measurement without manual intervention

CalScreener provides a label free method for measuring cellular metabolism. The direct detection of cellular bioenergy is a true phenotype analysis that is not limited by morphology or sample composition. It is a continuous measurement that monitors cell activity in real-time for any duration, without the need for any manual intervention.

Sensitivity and speed

Compared to optical analysis, the sensitivity of calScreener is approximately 1000 times higher, which enables monitoring of very small initial bacteria and obtaining results faster.

Accurately determine viable bacteria

The classic Pasteur type microbial plate test based on colony counting is prone to errors. Clusters of bacteria are often mistakenly identified as a single bacterium, leading to overestimation of drug efficacy. CalScreener can accurately measure live bacteria individually, which makes the measurement more accurate. Molecular based technologies also have the same problem, such as mass spectrometry and DNA amplification, which make it difficult to distinguish between live and dead cells.

It is easy to distinguish the mode of action of antibacterial and bactericidal agents

Continuous real-time measurement can easily determine whether a candidate drug works through bactericidal or bacteriostatic mechanisms.

Simple dose effect measurement

The calculation of total heat release can be used to easily determine the dose-response of candidate drugs without the need for further analytical experiments.

Determination of MIC (minimum inhibitory concentration)

The calorimetry method conforms to the CLSI standard MIC determination method and can be derived from the data.

Can monitor surviving/quiescent cells as well as regenerating cells over time

Retained/dormant bacteria that are difficult to detect by other methods are a major challenge in antibiotic development, but can be easily detected through calScreener. Because even small metabolic activities can be detected by calScreener, it provides the possibility to address the challenging issue in the study of antibiotic resistance mechanisms.

Specially suitable for monitoring bacteria that form biofilms and developing new antibacterial treatments

CalScreener can easily monitor the biofilm formation process of bacteria on a large number of possible substrates. This makes it particularly suitable for addressing this major obstacle in antibiotic resistance research. The vast majority of human infections have a special correlation with bacterial species that form biofilms. The development of new therapeutic methods and drugs targeting biofilm formation is an urgent need to maintain its leading position.

Unique bacterial identification based on growth patterns

Each specific bacterial strain and strain exhibits a unique growth pattern in the kinetic data. This can identify bacteria and detect contaminating microorganisms.

Detection and evaluation of the mechanism of action of antibiotic compounds

By comparing with antibiotics with known modes of action, the mechanism of action of exploratory drugs can be determined. Cell wall synthesis inhibition, DNA replication inhibition, and protein synthesis inhibition exhibit different inhibition modes, which can be used to understand the specific mode of action of uncharacterized substances.

toxicology

CalScreener technology hasspecialThe versatility of this device for drug screening can also be applied to mammalian cell toxicology testing of major candidate drugs, making the investment more cost-effective.