ZC3H14 protein antibody

ZC3H14 protein antibody

1. Specific immunogens

The type of immunogen used to produce antibodies significantly affects the specificity of antibodies to target proteins. Choosing antibodies produced against unsuitable or low-quality immunogens will result in unreliable and potentially irreproducible outcomes. Attention should be paid to selecting antibodies that target fully characterized immunogens.

2. Peptides

Synthetic peptide immunogens rarely fold into natural protein like tertiary structures. In addition, they do not exhibit any post-translational modifications unless added to the peptide during the synthesis process. Therefore, antibodies produced using peptides can be used as useful reagents for Western blotting, as target proteins have linearized epitopes due to denaturation and reduction during sample processing. When antibodies need to distinguish between two proteins with highly conserved amino acid sequences, peptides can also be used as immunogens. In these cases, the production of peptides to differential regions increases the likelihood of producing differential antibodies.

3. Natural protein

If purified proteins are used as immunogens, antibodies produced against them may not recognize the linear epitopes of denatured proteins in Western blotting. Some antibodies produced using natural proteins can only recognize natural oxidative conformational epitopes on the protein surface. This is especially true for monoclonal antibodies.

ZC3H14 belongs to a family of poly(A)-binding proteins that influence gene expression by regulating mRNA stability, nuclear export, and translation (Kelly et al., 2007 [PubMed 17630287])

1. Prepare antigens.

2. Select experimental animals.

3. Animal immunity.

4. Take blood samples for testing to see if immunization is successful.

5. If successfully immunized, kill the experimental animals and collect all serum.

6. Purify the antibody.

7. Identify antibodies. Including purity and specificity.
1） Antigen preparation

Ig is an immunoglobulin that is a good antigenic substance for heterologous animals. Can stimulate heterologous animals to produce anti Ig serum,

After appropriate extraction, anti Ig antibodies, also known as secondary antibodies or anti antibodies, are produced. In most indirect labeling reactions, preparation is required

Anti Ig antibody.

（2） Materials and reagents

1. Extracted animal Ig

2. Freund's adjuvant and Freund's non adjuvant

3. Experimental animal rabbit

4. Other materials and reagents

A3personTLymphocytic leukemia cellsThe A3 of human T lymphocyte leukemia cell RPMI-1640(GIBCO)+10%FBSRat tumor necrosis factor related apoptosis inducing ligand receptor4(AIL-R4)ELISAtest kitrHIL-1Ra/Cy3fluoresceinCy3mark-1Receptor antagonist0.1ml

LLGL2： Similar inhibitory genesHUGLantibodyCD2 Others HumanpersonCD2Human cell lysate(positive control)

Human lung fibroblastsHPFRat tumor necrosis factor related apoptosis inducing ligand receptor3(AIL-R3)ELISAtest kitKLH/Cy3fluoresceinCy3Mark hemocyanin0.1ml

LAMTOR1：LAMTOR1protein antibody Mouse embryonic osteoblast precursor cells;MC3T3-E1

B16-F1(Mouse melanoma cells) 5×106cells/bottle×2Human astrocytesHARat tumor necrosis factor related apoptosis inducing ligand receptor1(AIL-R1)ELISAtest kitOVA (Ovalbumin)/Cy3fluoresceinCy3Label chicken egg albumin0.1ml

Hurt-78Cells, humansTCell lymphoma Mouse embryonic fibroblasts,3T3-swiss albinocell Human alveolar epithelial cellscDNAHPAEpiC cDNAHuman soluble transreceptor(sTfR)ELISAtest kitDRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)N terminaldouble chainRNAAdenylate dehydrogenase antigen(Nend) 0.5mg

GNPTAB： Lysosomal accumulation disease-related proteins/Stuttering related protein antibodyPRLR Others MousemousePRLR / Prolactin ReceptorHuman cell lysate(positive control)

Normal rat kidney cells;NRKHuman soluble tumor necrosis factor related apoptosis inducing ligand(sAIL)ELISAtest kitDRADA/ADAR1(Double-stranded RNA-specific adenosine deaminase)double chainRNAAdenylate dehydrogenase antigen0.5mg

GSK3 Alpha + Beta： glycogen synthase kinase3α+βantibody Human embryonic kidney cells;2V6.11

COS-1(African Green MonkeySV40Transformed renal cells) 5×106cells/bottle×2 CL-0174NRK-52E(Rat renal tubular ductal epithelial cells)5×106cells/bottle×2Rat basophilic leukemia cells;RBL-2H3Human soluble tumor necrosis factoralphareceptor(sTNFαR)ELISAtest kitMMP9Matrix metalloproteinases9 0.5mg

ZC3H14protein antibodyPKA C alpha+beta： protein kinaseCantibodyJARCells, embryonic trophoblastic carcinoma cells Human lung adenocarcinoma cells,LTPEP-a-2cellKM3,Human multiple myeloma cells

LIF Others MousemouseLIFHuman cell lysate(positive control)Rat metallothionein(MT)ELISAtest kitSLE(Human systemic lupus erythematosus) Systemic lupus erythematosus in humans96T

PHF-tau： Paired spiral filament protein antibodyCL-0395NCI-H2227Human small cell lung cancer cells)5×106cells/bottle×2

SW620Human colon cancer➟煰➟