Z DNA binding protein antibody

Z DNA binding protein antibody

1. Prepare antigens.

2. Select experimental animals.

3. Animal immunity.

4. Take blood samples for testing to see if immunization is successful.

5. If successfully immunized, kill the experimental animals and collect all serum.

6. Purify the antibody.

7. Identify antibodies. Including purity and specificity.
1） Antigen preparation

Ig is an immunoglobulin that is a good antigenic substance for heterologous animals. Can stimulate heterologous animals to produce anti Ig serum,

After appropriate extraction, anti Ig antibodies, also known as secondary antibodies or anti antibodies, are produced. In most indirect labeling reactions, preparation is required

Anti Ig antibody.

（2） Materials and reagents

1. Extracted animal Ig

2. Freund's adjuvant and Freund's non adjuvant

3. Experimental animal rabbit

4. Other materials and reagents

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production.

A-431Human epidermal cancer cellsHuman epidermoid carcinoma A-431 cells DMEM+10%FBSRat osteoclast differentiation factor(ODF)ELISAtest kit VIP(Mouse Vasoactive Intestinal Peptide) Mouse vasoactive intestinal peptide96T

N acetylglucosamine kinase：N-Acetyl glucokinase antibodyCD274 Others HumanpersonB7-H1 / CD274 / PD-L1Human cell lysate(positive control)

CM-H052Human placental trophoblast cell culture medium100mLRat malate dehydrogenase1(MDH1)ELISAtest kitET Rat endotoxin96T

NFIB： nuclear factor1Bantibody Blackened rat lung fibroblasts;NRm

Human hippocampus astrocytes(HA-h)(1×106)Smooth muscle cells of human brain blood vesselsHumanRat flatfoot protein(PDPN)ELISAtest kitPPAR- Gamma Peroxisome proliferator activated receptor in ratsγ 96T

IDH3B： Isocitrate dehydrogenase3 betaSubunit antibodyMSC,Mouse Schwann cells Mouse retrovirus packaging cells,PT67cellP3/NSI/1-Ag4-1 [NS-1](myeloma cells)

EDA2R Others RatratXEDAR / EDA2RHuman cell lysate(positive control)Canine follicle stimulating hormone(FSH)ELISAtest kitNFKBp65(p65 NF-kappa B;p65NFKB)Nuclear factor, also known as nuclear factorkGene binding nuclear factor(Peptide antigen) 0.5mg

IFIT1： Interferon induced triangular tetrapeptide repeat protein1antibody Mouse melanoma tumor strain;B16

CFSC-3H & 5HCells, rat hepatic stellate cellsSK-N-SH(Neuroblastoma)Rat pituitary tumor cells;GH3Canine luteinizing hormone(LH)ELISAtest kitNR2A (Glutamate receptor2A)Glutamate receptor(NTerminal antigen) 0.5mg

IDH3G： Isocitrate dehydrogenasegammaSubunit antibodyCD200R1 Others Cynomolguscrab-eating macaqueCD200R / OX2-RHuman cell lysate(positive control)

Z DNABinding protein antibodyLX-2,Human hepatic stellate cell line ratαStransferase(α-GST)ELISAtest kitMYO/MB(Human Myoglobin) Human myoglobin96T

Phospho-RSK2 (Ser227)： 0Acidified ribosomeS6kinaseRSK2antibodyHA Others H7N9Influenza AH7N9 (A/Anhui/1/2013)hemagglutininHA1 (Hemagglutinin)Human cell lysate(positive control)

GHS1Golden hamster skin fibroblasts ratαStransferase(α-GST)ELISAtest kitcTn- I (Human cardiac t ➟煰➟

1. Specific immunogens

The type of immunogen used to produce antibodies significantly affects the specificity of antibodies to target proteins. Choosing antibodies produced against unsuitable or low-quality immunogens will result in unreliable and potentially irreproducible outcomes. Attention should be paid to selecting antibodies that target fully characterized immunogens.

2. Peptides

Synthetic peptide immunogens rarely fold into natural protein like tertiary structures. In addition, they do not exhibit any post-translational modifications unless added to the peptide during the synthesis process. Therefore, antibodies produced using peptides can be used as useful reagents for Western blotting, as target proteins have linearized epitopes due to denaturation and reduction during sample processing. When antibodies need to distinguish between two proteins with highly conserved amino acid sequences, peptides can also be used as immunogens. In these cases, the production of peptides to differential regions increases the likelihood of producing differential antibodies.

3. Natural protein

If purified proteins are used as immunogens, antibodies produced against them may not recognize the linear epitopes of denatured proteins in Western blotting. Some antibodies produced using natural proteins can only recognize natural oxidative conformational epitopes on the protein surface. This is especially true for monoclonal antibodies.