Yugong Biotechnology's rapid endonuclease NsiI, available in stock from Huicheng Biotechnology

NegotiableUpdate on 06/11

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Overview

Yugong Biotechnology's rapid endonuclease NsiI is available in stock from Huicheng Biotechnology, LightNing Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All LightNing Rapid endonucleases in the universal CutOne Or CutOne Color Buffer has excellent activity and can complete enzyme digestion within 5-15 minutes.

Product Details

Yugong Biotechnology's rapid endonuclease NsiI, available in stock from Huicheng Biotechnology

Product Introduction

LightNing ™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All LightNing ™ Rapid endonucleases in the universal CutOne ™ Or CutOne ™ Color Buffer has excellent activity and can complete enzyme digestion within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on CutOne ™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of "enzyme digestion modification connection".

CutOne ™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. CutOne ™ The migration rate of red dye of Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is close to each other; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Product Composition

component	specification
LightNing ™ NsiI	25 μl
10× CutOne™ Buffer	1 ml
10× CutOne™ Color Buffer	1 ml

特性和用法

Rapid endonuclease can complete the reaction within 5-15 minutes

Suggested reaction conditions

1× CutOne ™ Buffer solution;

Incubate at 37 ℃;

Prepare the reaction system according to the "DNA rapid enzyme digestion process".

Inactivation conditions

Incubate at 80 ℃ for 20 minutes.

Methylation sensitivity

For DNA methylated by EcoBI, splicing may be hindered.

Storage and Transportation Conditions

-20℃

Yugong Biotechnology's rapid endonuclease NsiI, available in stock from Huicheng Biotechnology

Shanghai Huicheng Biotechnology Co., Ltd. was established in 2010 with the corporate mission of "providing high-quality products and sincere services".Huicheng Biotechnology provides one-stop scientific services, covering the entire process of design and development, and offering specialized supporting services such as laboratory design, laboratory construction, instrument and equipment, consumables, reagents, and technical services. Huicheng Biotechnology has established a research and development center in Shanghai, production bases in Hubei and Jiangsu, and subsidiaries in Guangzhou and Shenzhen. Based in Shanghai, the sales network covers the whole country and provides comprehensive services to customers.

Huicheng Biotechnology specializes in providing various formal biochemical reagents, biochemical instruments, experimental consumables, various standard products, chromatographic accessories, etc., with over a thousand types of products and quantities exceeding 100000. Our professional product lines include sugar chemistry, lipid analysis, plant extraction, cytokines, molecular biology, and more. Our main customers are widely distributed in industries such as food, pharmaceuticals, in vitro diagnostics, agriculture, ecological environment, chemical industry, scientific research, biotechnology, and government testing institutions.