Yugong Biotechnology's rapid endonuclease NotI is available in stock from Huicheng Biotechnology

LightNing ™ Rapid endonucleases are a series of genetically engineered restriction endonucleases that are suitable for rapid enzymatic cleavage of plasmid DNA, PCR products, or genomic DNA. All LightNing ™ Rapid endonucleases in the universal CutOne ™ Or CutOne ™ Color Buffer has excellent activity and can complete enzyme digestion within 5-15 minutes. In addition, Baishimei dephosphorylation and ligation reagents are available on CutOne ™ Buffer has 100% activity and supports one tube reaction, enhancing the experience of "enzyme digestion modification connection".

CutOne ™ Color Buffer includes red and yellow tracer dyes, which can be directly used for gel electrophoresis. CutOne ™ The migration rate of red dye of Color Buffer and 2500 bp double stranded DNA fragment in 1% agarose gel is close to each other; The migration rate of yellow dye and 10 bp double stranded DNA fragment in 1% agarose gel is similar.

Rapid endonuclease can complete the reaction within 5-15 minutes

Suggested reaction conditions

1× CutOne ™ Buffer solution;

Incubate at 37 ℃;

Prepare the reaction system according to the "DNA rapid enzyme digestion process".

Inactivation conditions

Incubate at 80 ℃ for 20 minutes.

Methylation sensitivity

For DNA methylated by CpG, splicing may be hindered.

-20℃