Wnt signal inhibitor 1 antibody

Wnt signal inhibitor 1 antibody

1. Prepare antigens.

2. Select experimental animals.

3. Animal immunity.

4. Take blood samples for testing to see if immunization is successful.

5. If successfully immunized, kill the experimental animals and collect all serum.

6. Purify the antibody.

7. Identify antibodies. Including purity and specificity.
1） Antigen preparation

Ig is an immunoglobulin that is a good antigenic substance for heterologous animals. Can stimulate heterologous animals to produce anti Ig serum,

After appropriate extraction, anti Ig antibodies, also known as secondary antibodies or anti antibodies, are produced. In most indirect labeling reactions, preparation is required

Anti Ig antibody.

（2） Materials and reagents

1. Extracted animal Ig

2. Freund's adjuvant and Freund's non adjuvant

3. Experimental animal rabbit

4. Other materials and reagents

The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association

ANGPTL4 Others HumanpersonANGPTL4Human cell lysate(positive control)Rat inhibinβE(INHβE)ELISAtest kitLHLuteinizing hormone96T

Magea 11： Melanoma associated antigen11antibody Mouse embryonic fibroblasts;NIH/3T3

AE-1Hybridoma cell resistanceAChE AE-1 hybridoma cells against AChE DMEM+10% HycloneInactivated serum Rat inhibinβC(INHβC)ELISAtest kitFSHFollicle stimulating hormone96T

MAS1L：Gprotein coupled receptorMASRelated protein antibodiesRK3 Others MousemouseRK3 / kCHuman cell lysate(positive control)

Neural astrocytesMany types of cellsPacking:5 × 105power(1ml)Rat inhibinβA(INHβA)ELISAtest kitPLHprolactin96T

Nuclear Matrix Protein p84： Nuclear matrixp84protein antibodyCL-0422RBL-1(Rat basophilic leukemia cells)5×106cells/bottle×2

SK-N-SHCells, human neuroepithelial tumor cells Bacillus subtilis Human prostate epithelial cellsRNAHPEpiC miRNA5 μgHuman anti alveolar basement membrane antibody(ABM-Ab)ELISAtest kitNT-3nerve growth factor-3(antigen) 0.5mg

Centromeric histone H3-like protein-2： histoneH3Like antibodyHA Others H3N2Influenza AH3N2 (A/Aichi/2/1968)hemagglutinin(Hemagglutinin / HA)Human cell lysate(positive control)

Human nasopharyngeal carcinoma cells;CNE-2ZHuman anti spindle antibody(MSA)ELISAtest kitNT-4,NT-5nerve growth factor4/5(antigen) 0.5mg

Centromeric histone H3-like protein-1： histoneH3Like antibody Immortalized lymphocytes in humans;CNLMG-B5538LC

WntSignal suppression factor1antibodyphospho-PI 3 Kinase p110 delta (Tyr524)： 0acidification0Lipoacylinositol kinase catalytic subunitDantibodyCL-0112HMy2.CIR(personBLymphoblastic cell)5×106cells/bottle×2

SW-13Human adrenal cortex small cell carcinoma cellsSW-13 cells of small cell carcinoma of adrenal cortex L-15culture medium(GIBCO) + 10% FBSRat reactive oxygen species modulation factor1(ROMO1)ELISAtest kitTpP(Mouse thrombus precursor protein ➟煰➟

3

1. Specific immunogens

The type of immunogen used to produce antibodies significantly affects the specificity of antibodies to target proteins. Choosing antibodies produced against unsuitable or low-quality immunogens will result in unreliable and potentially irreproducible outcomes. Attention should be paid to selecting antibodies that target fully characterized immunogens.

2. Peptides

Synthetic peptide immunogens rarely fold into natural protein like tertiary structures. In addition, they do not exhibit any post-translational modifications unless added to the peptide during the synthesis process. Therefore, antibodies produced using peptides can be used as useful reagents for Western blotting, as target proteins have linearized epitopes due to denaturation and reduction during sample processing. When antibodies need to distinguish between two proteins with highly conserved amino acid sequences, peptides can also be used as immunogens. In these cases, the production of peptides to differential regions increases the likelihood of producing differential antibodies.

3. Natural protein

If purified proteins are used as immunogens, antibodies produced against them may not recognize the linear epitopes of denatured proteins in Western blotting. Some antibodies produced using natural proteins can only recognize natural oxidative conformational epitopes on the protein surface. This is especially true for monoclonal antibodies.