What is Phosphorylated Proteomics - Biotec Biotech

What is Phosphorylated Proteomics - Biotec BiotechIntroduction to Testing Technology Services Details

In living organisms, d à nb á izh ì is the main executor of life activities, and they maintain the normal functioning of the organism through interactions, regulation, and signal transmission. The function of d à nb á izh ì not only depends on its amino acid sequence, but is also regulated by post-translational modifications (PTM). Among them, phosphorylation, as a common and important post-translational modification, has a profound impact on the function and cellular signaling of d à nb á izh ì. Phosphorylated d d à nb á izh ì omics has emerged as a hot topic in the field of biology research.

Phosphorylation of d à nb á izh ì omics is a branch of d à nb á izh ì omics that mainly studies the phosphorylation modification of d à nb á izh ì in living organisms and its role in life processes. Phosphorylation refers to the addition of phosphate groups (PO4) to specific amino acid residues in d à nb á izh ì. Common phosphorylated amino acids include s īā nsu ā n (Ser), s ūā nsu ā n (Thr), and l à o ā nsu ā n (Tyr).

Phosphorylation has a significant impact on the function of d à nb á izh ì, as it can regulate the activity, stability, intracellular localization, and interactions between d à nb á izh ì. Phosphorylation plays a crucial role in biological processes such as cell signaling, metabolic regulation, and cell cycle control. Therefore, studying phosphorylated d à nb á izh ì omics can help reveal the essence of life phenomena and provide new ideas for disease diagnosis and treatment.

1、 The main steps of the phosphorylation d à nb á izh ì omics research method

1. Sample preparation: The primary step in phospho d à nb á izh ì omics research is sample preparation. This includes cell lysis, d à nb á izh ì extraction, phosphorylation d à nb á izh ì enrichment, etc. Due to the low content of phosphorylated d à nb á izh ì in cells, the enrichment step is particularly important; D à nb á izh ì separation. The extracted d à nb á izh ì was separated by gel electrophoresis, liquid chromatography and other technologies to facilitate subsequent mass spectrometry analysis.

2. Mass spectrometry analysis: Mass spectrometry (MS) is the core technology for studying phosphorylated d à nb á izh ì omics. Through mass spectrometry analysis, phosphorylation of d à nb á izh ì and its phosphorylation sites can be identified and quantified. In recent years, the development of tandem mass spectrometry (MS/MS) technology has greatly improved the research level of phosphorylated d à nb á izh ì omics.

3. Data analysis: Use bioinformatics tools to analyze mass spectrometry data, identify phosphorylation patterns, and analyze the functions and interaction networks of phosphorylated d à nb á izh ì.

2、 Application of Phosphorylated D à nb á izh ì omics

1. Disease research: Phosphorylated d à nb á izh ì omics plays an important role in disease research. By comparing the phosphorylation differences between normal cells and diseased cells, key regulatory factors related to diseases can be identified, providing targets for disease diagnosis and treatment.

2. Drug development: Phosphorylated d à nb á izh ì omics can help discover new drug targets. Drug design targeting phosphorylation of d à nb á izh ì can more effectively regulate disease-related signaling pathways.

3. Systems Biology: Phosphorylated d à nb á izh ì omics provides abundant data resources for systems biology research. By analyzing the phosphorylation d à nb á izh ì network, the intracellular signaling mechanism can be revealed, providing a new perspective for biological research.

Phosphorylated d d à nb á izh ì omics has achieved significant results, but still faces many challenges. The dynamic range, low abundance, and high heterogeneity of phosphorylation d à nb á izh ì, as well as the complex data analysis in mass spectrometry analysis. As an emerging discipline, phosphogenomics has broad application prospects. Through the study of phosphorylation of d à nb á izh ì, we will better reveal the mysteries of life and contribute to the cause of human health.

Baitai Paike Biotechnology Characteristic Project

1、 Protein sequencing

Biotec Biotech uses Thermo's newly launched Obitrap Fusion Lumos mass spectrometer and Shimadzu's Edman degradation sequencing system to analyze the d à nb á izh ì sequence, providing mass spectrometry based protein sequencing analysis services, including amino acid composition analysis of d à nb á izh ì, Ndu ā nc è x ù, Cdu ā nc è x ù, and full sequence analysis, as well as d à nb á izh ì N-terminal sequence analysis services based on Edman degradation. For unknown theoretical sequences, we provide de novo sequencing services based on de novo sequencing to analyze protein sequences.

※ Service advantages:

1. Adopting the world's advanced mass spectrometer Obitrap Fusion Lumos;

2. It can achieve 100% coverage of the measured target protein sequence;

3. It can determine up to 70 amino acid sequences at the N-terminus of proteins;

4. Can measure various forms of samples: protein solution, PVDF protein bands;

5. Low sample usage: Protein samples only require 5-10ug to complete detection;

6. Sequencing is not affected by N-terminal modifications such as blocking, PEC, and glycosylation.

2、 D à nb á izh ì omics

Biotec Biotech uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with Nano LC to provide a variety of d à nb á izh ì omics analysis services, including quantitative d à nb á izh ì omics, targeted d à nb á izh ì omics, proteomics, post-translational modified proteomics, and more. In addition, Baitai Paike Biotechnology has launched a 4DD à nb á izh ì omics service based on timsTOF Pro, which helps with research in micro sample proteomics, large sample population medicine, and high-throughput modification genomics.

※ Service advantages:

1 . High throughput quantitative protein analysis: Large scale experimental analysis with multiple control groups, discovering new biomarkers;

2. Multiple in vitro and in vivo labeling methods are suitable for analyzing various samples such as tissues, cells, and blood;

3. High sensitivity of mass spectrometry analysis and high repeatability of experimental results;

4. Can detect low abundance proteins with a wide linear range;

5. Bioinformatics analysis is more systematic and accurate.

3、 Single cell mass spectrometry flow cytometry analysis

Baitai Paike Biotechnology uses the Fluidigm mass spectrometry flow cytometry system for single-cell mass spectrometry analysis. Metal element markers (usually metal element labeled specific antibodies) are used to label molecules on the surface and inside of cells. Then, individual cells are separated using flow cytometry principles, and the atomic mass spectrum of individual cells is analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The atomic mass spectrum data is then converted into signal molecule expression levels on the cell surface and inside.

※ Service advantages:

1. Advanced technology, filling technological gaps

The use of metal labeled antibody technology avoids the problem of few and easily interacting channels in traditional flow cytometry fluorescence. At the single-cell level, multiple indicators can be characterized simultaneously, and Baitai Paike Biotechnology can detect 51 target proteins simultaneously.

2. Large analysis quantity and low cost

Single cell RNAseq is limited by factors such as cost, and the total number of analyzed cells for all samples is generally around 2x10 ^ 4. However, flow cytometry technology can analyze at least 10 ^ 5 cells in one sample, achieving an order of magnitude increase, and the cost is not higher than that of single-cell RNAseq.

3. Great application prospects

① The results of flow cytometry can provide changes in cell subpopulations, which have great research prospects in clinical diagnosis, disease mechanism research, and other fields;

② Combining metal tag technology with other technologies will lead to new application directions. In addition to conventional proteins, mass spectrometry flow cytometry technology can also be used for post-translational modification of proteins;

③ Can detect cell survival rate, cell size, mRNA transcript expression level, DNA synthesis rate, and protease activity.

4、 Immunopeptide omics analysis and novel antigen discovery based on high-precision mass spectrometry

The one-stop solution for high-precision mass spectrometry based immunopeptidomic analysis and new antigen discovery from Biotec Biotech includes our proprietary, highly sensitive immunopeptide enrichment and identification scheme. We can assist you in identifying and recognizing over 10000 type I peptides and over 10000 type II peptides. Through our optimized high-throughput immunopeptidomic analysis platform, immunopeptidomic analysis can be performed with reproducible identification and quantification from small sample materials. This service can be applied to large-scale research, aiming to assist researchers in finding solutions for cancer, immune diseases, and infectious diseases, and to explore unknown targets in depth.

5、 Characterization of Biopharmaceuticals

Baitai Parker is based on high-resolution mass spectrometry technology, MALDI TOF， High performance chromatography separation technology provides a range of biological drug analysis solutions, from amino acid composition and primary structure analysis of biological products such as d à nb á izh ì, peptides, antibodies, vaccines, to product variability and purity analysis. Intended to provide high-quality biopharmaceutical analysis services and assist biopharmaceutical manufacturers in improving the quality of biopharmaceuticals.

Baitai Paike Biotechnology's Seven Major Testing Platforms

Baitai Paike Biotechnology - High quality service provider for characterization of biological products and multi omics of biological mass spectrometry

Beijing Baitai Paike Biotechnology Co., Ltd. is committed to providing quality control testing and project validation services for the biological/pharmaceutical and medical device industries. The company's laboratory follows regulations and guidelines such as NMPA, ICH, FDA, and EMA, and has obtained CNAS/ISO9001 dual quality system certification. It has established a complete quality system, including data cold and hot/remote backup, regular equipment measurement/periodic verification, software audit tracking, and provides customers with integrated solutions and technical services to support new drug research and development, drug application registration, and production release.

1. The company adopts the ISO9001 quality control system and provides CRO detection and analysis services based on mass spectrometry;

2. Recognized by the National CNAS Laboratory, we provide customers with drug quality research services that comply with global pharmaceutical regulations;

3. The business scope covers omics, proteomics, metabolomics, biopharmaceutical characterization, single-cell analysis, single-cell mass spectrometry flow cytometry, bioinformatics cloud analysis, and multi omics integrated analysis of biological mass spectrometry;

4. Seven major quality control testing platforms to meet your one-stop service needs;

5. Providing services to over 3000 enterprises and 10000+customers;

6. Committed to providing you with high-quality biological mass spectrometry analysis services!

What is Phosphorylated Proteomics - Biotec BiotechIntroduction to Testing Technology Services Details