Can mass spectrometry determine protein structure - Biotec Biotech

Can mass spectrometry determine protein structure - Biotec BiotechIntroduction to Testing Technology Services Details

In the field of d à nb á izh ì research, determining protein structures is crucial for understanding their functions, interactions, and roles in diseases. Mass spectrometry technology, as an analytical tool, plays an increasingly important role in the study of d à nb á izh ì. So, can mass spectrometry determine the structure of d à nb á izh ì?

Traditionally, X-ray crystallography and nuclear magnetic resonance (NMR) have been the main methods for determining protein structures. X-ray crystallography can provide high-resolution three-dimensional structures of d à nb á izh ì, but it requires d à nb á izh ì crystallization, and many d à nb á izh ì are difficult to crystallize, limiting the application scope of this method. NMR can be used for protein structure determination in solution, but there are certain limitations for the study of high molecular weight d à nb á izh ì.

Mass spectrometry technology has advantages in protein structure research. Mass spectrometry can determine the molecular weight of d à nb á izh ì, and by measuring the molecular weight of d à nb á izh ì and its enzymatic fragments, the amino acid sequence information of d à nb á izh ì can be obtained. For example, by using electrospray ionization (ESI) mass spectrometry and matrix assisted laser desorption ionization (MALDI) mass spectrometry, we can ionize d à nb á izh ì and measure its mass charge ratio, and then calculate the molecular weight. In the study of d à nb á izh ì omics, molecular weight determination and sequence analysis of a large number of d à nb á izh ì can be performed through mass spectrometry, enabling high-throughput identification of d à nb á izh ì.

Mass spectrometry can also provide detailed information on the modification of d à nb á izh ì. Post translational modifications such as phosphorylation and glycosylation play a key role in its functional regulation. Mass spectrometry can determine the modification site and type by detecting the changes in molecular weight of d à nb á izh ì before and after modification. By using tandem mass spectrometry (MS/MS) technology to further cleave and analyze the peptide segments hydrolyzed by d à nb á izh ì enzyme, the modification sites can be located, providing strong evidence for studying the relationship between d à nb á izh ì modification and function.

In recent years, mass spectrometry has also made significant progress in the study of the structure of d à nb á izh ì g ā oj í. Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful tool for studying the conformation and kinetics of d à nb á izh ì. The hydrogen atoms in d à nb á izh ì have different exchange rates with deuterium atoms in different environments. By measuring the molecular weight changes of peptide segments before and after exchange through mass spectrometry, information about the secondary and tertiary structures of d à nb á izh ì and the interactions between structural domains can be inferred. For example, when studying the binding of d à nb á izh ì with small molecule drugs, HDX-MS can reveal the drug binding site and the resulting conformational changes in d à nb á izh ì, providing important structural information for drug development.

However, the determination of protein structure by mass spectrometry also has certain limitations. Although it can provide rich sequence, modification, and partial g ā oj í structural information, it is currently difficult to rely solely on mass spectrometry for the complete atomic resolution three-dimensional structure determination of d à nb á izh ì. It is more often used as a supplementary tool, combined with techniques such as X-ray crystallography and NMR, to jointly analyze protein structures. For example, in the study of m ó d à nb á i structure, due to the difficulty of crystallization, mass spectrometry can be used for sequence analysis, modification identification, and preliminary structural domain research, and then combined with other techniques to determine the complete three-dimensional structure.

Mass spectrometry technology plays a crucial role in protein structure research. It can provide sequence, modification, and partial g ā oj í structural information of d à nb á izh ì. Although it is challenging to determine the complete atomic resolution three-dimensional structure of d à nb á izh ì alone, when combined with other technologies, it can greatly promote the progress of protein structure research, provide strong support for deepening the understanding of d à nb á izh ì function, overcoming related diseases, and developing new biological drugs. It occupies a prominent position in the field of modern d à nb á izh ì science.

Baitai Paike Biotechnology Characteristic Project

1、 Protein sequencing

Biotec Biotech uses Thermo's newly launched Obitrap Fusion Lumos mass spectrometer and Shimadzu's Edman degradation sequencing system to analyze the d à nb á izh ì sequence, providing mass spectrometry based protein sequencing analysis services, including amino acid composition analysis of d à nb á izh ì, Ndu ā nc è x ù, Cdu ā nc è x ù, and full sequence analysis, as well as d à nb á izh ì N-terminal sequence analysis services based on Edman degradation. For unknown theoretical sequences, we provide de novo sequencing services based on de novo sequencing to analyze protein sequences.

※ Service advantages:

1. Adopting the world's advanced mass spectrometer Obitrap Fusion Lumos;

2. It can achieve 100% coverage of the measured target protein sequence;

3. It can determine up to 70 amino acid sequences at the N-terminus of proteins;

4. Can measure various forms of samples: protein solution, PVDF protein bands;

5. Low sample usage: Protein samples only require 5-10ug to complete detection;

6. Sequencing is not affected by N-terminal modifications such as blocking, PEC, and glycosylation.

2、 D à nb á izh ì omics

Biotec Biotech uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with Nano LC to provide a variety of d à nb á izh ì omics analysis services, including quantitative d à nb á izh ì omics, targeted d à nb á izh ì omics, proteomics, post-translational modified proteomics, and more. In addition, Baitai Paike Biotechnology has launched a 4DD à nb á izh ì omics service based on timsTOF Pro, which helps with research in micro sample proteomics, large sample population medicine, and high-throughput modification genomics.

※ Service advantages:

1 . High throughput quantitative protein analysis: Large scale experimental analysis with multiple control groups, discovering new biomarkers;

2. Multiple in vitro and in vivo labeling methods are suitable for analyzing various samples such as tissues, cells, and blood;

3. High sensitivity of mass spectrometry analysis and high repeatability of experimental results;

4. Can detect low abundance proteins with a wide linear range;

5. Bioinformatics analysis is more systematic and accurate.

3、 Single cell mass spectrometry flow cytometry analysis

Baitai Paike Biotechnology uses the Fluidigm mass spectrometry flow cytometry system for single-cell mass spectrometry analysis. Metal element markers (usually metal element labeled specific antibodies) are used to label molecules on the surface and inside of cells. Then, individual cells are separated using flow cytometry principles, and the atomic mass spectrum of individual cells is analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The atomic mass spectrum data is then converted into signal molecule expression levels on the cell surface and inside.

※ Service advantages:

1. Advanced technology, filling technological gaps

The use of metal labeled antibody technology avoids the problem of few and easily interacting channels in traditional flow cytometry fluorescence. At the single-cell level, multiple indicators can be characterized simultaneously, and Baitai Paike Biotechnology can detect 51 target proteins simultaneously.

2. Large analysis quantity and low cost

Single cell RNAseq is limited by factors such as cost, and the total number of analyzed cells for all samples is generally around 2x10 ^ 4. However, flow cytometry technology can analyze at least 10 ^ 5 cells in one sample, achieving an order of magnitude increase, and the cost is not higher than that of single-cell RNAseq.

3. Great application prospects

① The results of flow cytometry can provide changes in cell subpopulations, which have great research prospects in clinical diagnosis, disease mechanism research, and other fields;

② Combining metal tag technology with other technologies will lead to new application directions. In addition to conventional proteins, mass spectrometry flow cytometry technology can also be used for post-translational modification of proteins;

③ Can detect cell survival rate, cell size, mRNA transcript expression level, DNA synthesis rate, and protease activity.

4、 Immunopeptide omics analysis and novel antigen discovery based on high-precision mass spectrometry

The one-stop solution for high-precision mass spectrometry based immunopeptidomic analysis and new antigen discovery from Biotec Biotech includes our proprietary, highly sensitive immunopeptide enrichment and identification scheme. We can assist you in identifying and recognizing over 10000 type I peptides and over 10000 type II peptides. Through our optimized high-throughput immunopeptidomic analysis platform, immunopeptidomic analysis can be performed with reproducible identification and quantification from small sample materials. This service can be applied to large-scale research, aiming to assist researchers in finding solutions for cancer, immune diseases, and infectious diseases, and to explore unknown targets in depth.

5、 Characterization of Biopharmaceuticals

Baitai Parker is based on high-resolution mass spectrometry technology, MALDI TOF， High performance chromatography separation technology provides a range of biological drug analysis solutions, from amino acid composition and primary structure analysis of biological products such as d à nb á izh ì, peptides, antibodies, vaccines, to product variability and purity analysis. Intended to provide high-quality biopharmaceutical analysis services and assist biopharmaceutical manufacturers in improving the quality of biopharmaceuticals.

Baitai Paike Biotechnology's Seven Major Testing Platforms

Baitai Paike Biotechnology - High quality service provider for characterization of biological products and multi omics of biological mass spectrometry

Beijing Baitai Paike Biotechnology Co., Ltd. is committed to providing quality control testing and project validation services for the biological/pharmaceutical and medical device industries. The company's laboratory follows regulations and guidelines such as NMPA, ICH, FDA, and EMA, and has obtained CNAS/ISO9001 dual quality system certification. It has established a complete quality system, including data cold and hot/remote backup, regular equipment measurement/periodic verification, software audit tracking, and provides customers with integrated solutions and technical services to support new drug research and development, drug application registration, and production release.

1. The company adopts the ISO9001 quality control system and provides CRO detection and analysis services based on mass spectrometry;

2. Recognized by the National CNAS Laboratory, we provide customers with drug quality research services that comply with global pharmaceutical regulations;

3. The business scope covers omics, proteomics, metabolomics, biopharmaceutical characterization, single-cell analysis, single-cell mass spectrometry flow cytometry, bioinformatics cloud analysis, and multi omics integrated analysis of biological mass spectrometry;

4. Seven major quality control testing platforms to meet your one-stop service needs;

5. Providing services to over 3000 enterprises and 10000+customers;

6. Committed to providing you with high-quality biological mass spectrometry analysis services!

Can mass spectrometry determine protein structure - Biotec BiotechIntroduction to Testing Technology Services Details