What is LC-MS molecular weight analysis? Applicable subjects

What is LC-MS molecular weight analysis? Applicable subjectsIntroduction to Methods and Their Advantages and Disadvantages Comparison Testing Technology Service Details

Molecular weight is one of the fundamental and important physicochemical parameters of biomolecules. Accurately obtaining molecular weight information is not only a prerequisite for structural analysis, but also the foundation for quality control and functional research in many fields of life sciences, such as d à nb á izh ì engineering, antibody drug development, synthetic compound validation, and metabolomics analysis. Liquid Chromatography Mass Spectrometry (LC-MS), as a highly sensitive, high-throughput, and analytical tool capable of resolving different molecular weight components in complex samples, is increasingly becoming the mainstream choice in the fields of scientific research and biopharmaceuticals.

1、 What is LC-MS molecular weight analysis?

LC-MS molecular weight analysis is a method that combines liquid chromatography separation with mass spectrometry detection to separate, detect, and determine the relative molecular weight of target molecules in complex samples. Specifically:

• Liquid chromatography (LC) is used to separate various components in a sample based on their physical and chemical properties.

• Mass spectrometry (MS) ionizes each separated component and measures its mass to charge ratio (m/z) to infer its molecular weight.

Compared with traditional UV detection or gel electrophoresis, LC-MS does not depend on specific dyes or standards, and can be used to characterize unknown structures or micro samples.

2、 Applicable objects of LC-MS molecular weight analysis

LC-MS can be applied to almost all organic or biomolecules with molecular weights ranging from 100 Da to several hundred kDa. The following are common application objects:

1. Recombinant proteins and antibody drugs

• Confirm if the molecular weight of the expressed product is correct.

• Verify whether post-translational modifications (such as glycosylation, acetylation, etc.) have occurred.

• Monitor isomers, degradation products, or aggregates.

2. Synthesis of small molecules or compounds

• Confirm the integrity of molecular structure synthesis.

• Detect residual by-products or intermediates.

• Conduct qualitative/semi quantitative analysis.

3. Peptides and nucleic acid drugs

• Peptides with molecular weight less than 10 kDa are suitable for rapid molecular weight confirmation using ESI-MS or MALDI-MS.

• Anti sense oligonucleotides, siRNA, etc. can also be detected for their integrity and modification status by LC-MS.

4. Metabolomics and Lipids

• High throughput determination of thousands of metabolites/lipid molecules.

• Provide accurate molecular formula information to assist in compound identification and functional analysis.

3、 Common methods for LC-MS molecular weight analysis

Different LC-MS procedures will be used for different analysis purposes and sample types. The following are several mainstream strategies currently in use:

1. Desalination+ESI-MS analysis (applicable to proteins and peptides)

• Often combined with online or offline desalination (such as C4/C18 sample pretreatment).

• Electro spray ionization (ESI) is adopted, which is suitable for macromolecules with multiple charges.

• Use software deconvolution algorithm to obtain the single molecular weight of proteins.

Advantages: High sensitivity, molecular weight resolution.

Limitations: Sensitive to salt concentration, sample buffer needs to be optimized.

2. LC-MS full scan analysis (applicable to complex mixed samples)

• Use gradient elution liquid phase system for component separation.

• Combined with high-resolution mass spectrometers such as TOF, Orbitrap, or Q-Exactive.

• Suitable for screening molecular weight characteristic peaks in complex backgrounds.

Advantages: High throughput, suitable for unknown sample analysis.

Limitations: Database matching required, slightly longer analysis time.

3. MALDI-TOF molecular weight analysis (applicable to small molecules and peptides)

• Directly detect samples using matrix assisted laser desorption ionization (MALDI) method.

• Suitable for fast and high-throughput molecular weight confirmation.

Advantages: Fast analysis speed, strong salt resistance of samples.

Limitations: Not suitable for high molecular weight proteins, uneven ionization efficiency affects quantification.

4、 LC-MS molecular weight analysis vs. other molecular weight detection methods

The following is a comparative overview of common molecular weight detection methods and LC-MS:

It can be seen that LC-MS is not suitable for all scenarios, but it has advantages in molecular weight confirmation, complex sample analysis, high-resolution qualitative analysis, etc.

5、 How does Baitai Paike Biotechnology provide LC-MS molecular weight analysis services?

At Baitai Paike Biotechnology, we integrate high-resolution Orbitrap mass spectrometry platforms (Q-Exactive HF-X, Exploris 480, etc.) with various sample pretreatment processes to provide highly sensitive, rapid, and customizable molecular weight analysis services for research clients and biopharmaceutical companies

• Supports various types of samples such as proteins, peptides, synthetic small molecules, oligonucleotides, etc.

• Provide full process support for sample desalination, buffer replacement, deconvolution data processing, etc.

• Provide standardized reports, including TIC plots, m/z spectra, deconvolution results, etc.

• Can be combined with protein N-terminal/C du ā nc è x ù, glycosylation modification analysis and other service linkage.

We are committed to providing customers with mass spectrometry data that is not only "usable" but also "easy to use", to help your scientific research and product development progress more efficiently.

LC-MS molecular weight analysis has become an accurate, efficient, and scalable tool in current life science research and industrial applications. Its performance in various molecular types and complex sample backgrounds far exceeds the limitations of traditional methods. LC-MS is an important guarantee for improving data reliability and experimental efficiency, whether used for scientific research validation or quality control in new drug development. If you are looking for high-throughput molecular weight analysis services, please feel free to contact Baitai Paike Biotechnology. We will provide you with high-quality technology platforms and data interpretation capabilities to safeguard your scientific research.

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Baitai Paike Biotechnology Characteristic Project

1、 Protein sequencing

Biotec Biotech uses Thermo's newly launched Obitrap Fusion Lumos mass spectrometer and Shimadzu's Edman degradation sequencing system to analyze the d à nb á izh ì sequence, providing mass spectrometry based protein sequencing analysis services, including amino acid composition analysis of d à nb á izh ì, Ndu ā nc è x ù, Cdu ā nc è x ù, and full sequence analysis, as well as d à nb á izh ì N-terminal sequence analysis services based on Edman degradation. For unknown theoretical sequences, we provide de novo sequencing services based on de novo sequencing to analyze protein sequences.

※ Service advantages:

1. Adopting the world's advanced mass spectrometer Obitrap Fusion Lumos;

2. It can achieve 100% coverage of the measured target protein sequence;

3. It can determine up to 70 amino acid sequences at the N-terminus of proteins;

4. Can measure various forms of samples: protein solution, PVDF protein bands;

5. Low sample usage: Protein samples only require 5-10ug to complete detection;

6. Sequencing is not affected by N-terminal modifications such as blocking, PEC, and glycosylation.

2、 D à nb á izh ì omics

Biotec Biotech uses Thermo Fisher's Orbitrap Fusion Lumos mass spectrometry platform combined with Nano LC to provide a variety of d à nb á izh ì omics analysis services, including quantitative d à nb á izh ì omics, targeted d à nb á izh ì omics, proteomics, post-translational modified proteomics, and more. In addition, Baitai Paike Biotechnology has launched a 4DD à nb á izh ì omics service based on timsTOF Pro, which helps with research in micro sample proteomics, large sample population medicine, and high-throughput modification genomics.

※ Service advantages:

1 . High throughput quantitative protein analysis: Large scale experimental analysis with multiple control groups, discovering new biomarkers;

2. Multiple in vitro and in vivo labeling methods are suitable for analyzing various samples such as tissues, cells, and blood;

3. High sensitivity of mass spectrometry analysis and high repeatability of experimental results;

4. Can detect low abundance proteins with a wide linear range;

5. Bioinformatics analysis is more systematic and accurate.

3、 Single cell mass spectrometry flow cytometry analysis

Baitai Paike Biotechnology uses the Fluidigm mass spectrometry flow cytometry system for single-cell mass spectrometry analysis. Metal element markers (usually metal element labeled specific antibodies) are used to label molecules on the surface and inside of cells. Then, individual cells are separated using flow cytometry principles, and the atomic mass spectrum of individual cells is analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The atomic mass spectrum data is then converted into signal molecule expression levels on the cell surface and inside.

※ Service advantages:

1. Advanced technology, filling technological gaps

The use of metal labeled antibody technology avoids the problem of few and easily interacting channels in traditional flow cytometry fluorescence. At the single-cell level, multiple indicators can be characterized simultaneously, and Baitai Paike Biotechnology can detect 51 target proteins simultaneously.

2. Large analysis quantity and low cost

Single cell RNAseq is limited by factors such as cost, and the total number of analyzed cells for all samples is generally around 2x10 ^ 4. However, flow cytometry technology can analyze at least 10 ^ 5 cells in one sample, achieving an order of magnitude increase, and the cost is not higher than that of single-cell RNAseq.

3. Great application prospects

① The results of flow cytometry can provide changes in cell subpopulations, which have great research prospects in clinical diagnosis, disease mechanism research, and other fields;

② Combining metal tag technology with other technologies will lead to new application directions. In addition to conventional proteins, mass spectrometry flow cytometry technology can also be used for post-translational modification of proteins;

③ Can detect cell survival rate, cell size, mRNA transcript expression level, DNA synthesis rate, and protease activity.

4、 Immunopeptide omics analysis and novel antigen discovery based on high-precision mass spectrometry

The one-stop solution for high-precision mass spectrometry based immunopeptidomic analysis and new antigen discovery from Biotec Biotech includes our proprietary, highly sensitive immunopeptide enrichment and identification scheme. We can assist you in identifying and recognizing over 10000 type I peptides and over 10000 type II peptides. Through our optimized high-throughput immunopeptidomic analysis platform, immunopeptidomic analysis can be performed with reproducible identification and quantification from small sample materials. This service can be applied to large-scale research, aiming to assist researchers in finding solutions for cancer, immune diseases, and infectious diseases, and to explore unknown targets in depth.

5、 Characterization of Biopharmaceuticals

Baitai Parker is based on high-resolution mass spectrometry technology, MALDI TOF， High performance chromatography separation technology provides a range of biological drug analysis solutions, from amino acid composition and primary structure analysis of biological products such as d à nb á izh ì, peptides, antibodies, vaccines, to product variability and purity analysis. Intended to provide high-quality biopharmaceutical analysis services and assist biopharmaceutical manufacturers in improving the quality of biopharmaceuticals.

Baitai Paike Biotechnology's Seven Major Testing Platforms

Baitai Paike Biotechnology - High quality service provider for characterization of biological products and multi omics of biological mass spectrometry

Beijing Baitai Paike Biotechnology Co., Ltd. is committed to providing quality control testing and project validation services for the biological/pharmaceutical and medical device industries. The company's laboratory follows regulations and guidelines such as NMPA, ICH, FDA, and EMA, and has obtained CNAS/ISO9001 dual quality system certification. It has established a complete quality system, including data cold and hot/remote backup, regular equipment measurement/periodic verification, software audit tracking, and provides customers with integrated solutions and technical services to support new drug research and development, drug application registration, and production release.

1. The company adopts the ISO9001 quality control system and provides CRO detection and analysis services based on mass spectrometry;

2. Recognized by the National CNAS Laboratory, we provide customers with drug quality research services that comply with global pharmaceutical regulations;

3. The business scope covers omics, proteomics, metabolomics, biopharmaceutical characterization, single-cell analysis, single-cell mass spectrometry flow cytometry, bioinformatics cloud analysis, and multi omics integrated analysis of biological mass spectrometry;

4. Seven major quality control testing platforms to meet your one-stop service needs;

5. Providing services to over 3000 enterprises and 10000+customers;

6. Committed to providing you with high-quality biological mass spectrometry analysis services!

What is LC-MS molecular weight analysis? Applicable subjectsIntroduction to Methods and Their Advantages and Disadvantages Comparison Testing Technology Service Details