Vibrio cholerae detection kit (colloidal gold method)

Common name:Vibrio cholerae assay kit(Colloidal gold method)

01 group test strips: 25 servings/box; 0139 group test strips: 25 servings/box

This product is used forRegarding fecesSample processing01 and 0139 Vibrio choleraeQualitative testing.

【 Principle of Inspection 】

This reagent kit uses colloidal gold immunochromatographic double antibody sandwich method to detect the presence of antibodies in specimensVibrio cholerae01, 0139 group antigens（Ag）， Using colloidal gold as a marker and nitrocellulose membrane as a coating carrier. The nitrocellulose membrane is coated with two types of antibodies, one of which is antiVibrio cholerae01 or 0139 groupMonoclonal antibodies (Ab) are used to capture antigens in specimens; Another type is sheep anti mouse multi antibody, used for quality control of this test strip. Combined with other raw materials, assemble into test strips. During the detection process, the specimen is moved by the chromatographic action of the microporous membrane, and antigen antibody reactions occur during the movement. If the specimen containsVibrio cholerae01 or 0139 groupFirstly, it binds with colloidal gold labeled antibody (Au Ab) to form an Au Ab Ag complex. When the Au Ab Ag complex encounters the antibody coated on the membrane, it forms an Au Ab Ag Ab double antibody sandwich complex, presenting a visible red band.

If not present in the specimen01 or 0139 Vibrio choleraeThis area will not show color. Sheep anti mouse multi antibody zone, regardless of whether it is present in the sample or not01 or 0139 Vibrio choleraeAll display red, which serves as a marker for determining whether the test strip has failed or not.

Main components

Vibrio cholerae01 Group detection test stripThere are detection and quality control lines on the nitrocellulose membrane of the test strip.

Testing Line: Coating Resistance01 Vibrio cholerae groupMonoclonal antibody;

Quality control line: coated sheep anti mouse multi antibody;

Colloidal gold labeled antibody: anti01 Vibrio cholerae groupMonoclonal antibody.

Vibrio cholerae0139 group detection test stripsThere are detection and quality control lines on the nitrocellulose membrane of the test strip.

Testing Line: Coating ResistanceVibrio cholerae group 0139Monoclonal antibody;

Quality control line: coated sheep anti mouse multi antibody;

Colloidal gold labeled antibody: antiVibrio cholerae group 0139Monoclonal antibody.

Storage conditions and expiration date

Store at room temperature (4-30 ℃) and have a shelf life of 18 months.

Sample type:feces.

Sample processing:

• Direct testing: Water samples are directly tested.2. Bacterial enrichment detection

Water sample stool:Inoculate 1-2 ml in 8-9 ml alkaline peptone water and incubate at 37 ℃ for 6-8 hours or overnight.

Solid fecal specimens: Inoculate 1-2g in 8-9ml alkaline peptone water, mix well, and incubate at 37 ℃ for 6-8 hours or overnight.

1. Before testing, the sample and test strip should be equilibrated to room temperature;

2. Place the test strip on a clean and dry workbench;

3. Take 2-3 drops of the sample using the pipette provided in this kit or a clean sample dispenser, and add them to the sample addition end of the test strip (white end of the test strip) to start timing;

4. Observation results for 2-15 minutes, reading results for 15 minutes or more is invalid.

Explanation of Inspection Results

Negative: A red line appears, indicating the presence of a red line at the position of the quality control line;

Positive: Two red lines appear, indicating the presence of red lines at both the detection and quality control lines;

invalid

• No red lines appear at the positions of the detection line and quality control line.

• The quality control line has not been released, only the detection line shows a red line.

• Vibrio cholerae detection kit (colloidal gold method)