Valley Biomedical/BioIVT Human AB Serum

Background Introduction

Normal human AB serum is collected using AB blood type (the latest and rarest blood type discovered, accounting for only about 5% of the total population), and is made from normal human blood through sterile collection, separation, and microporous filtration. This product is free from mycoplasma, viruses, and phages, with an endotoxin content of less than 1EU/ml. It is suitable for cell and tissue organ culture, cell strain preservation, monoclonal antibody development, and T cells. It is one of the important culture media for hospitals, research institutions, animal vaccines, and biopharmaceutical manufacturers. It is also applicable to animal immunity, serum IgG preparation, pathological histological blockade, ELISA, and so on. The difference between plasma and serum is that serum does not contain fibrinogen, etc. Plasma is the liquid component of blood, in which blood cells are suspended.

The human body contains 2750-3300 milliliters of plasma, accounting for approximately 55% of the total blood volume. The vast majority of plasma is water (90% by volume), and the dissolved substances are mainly plasma proteins, as well as glucose, inorganic salt ions, hormones, and carbon dioxide. The main function of plasma is to transport blood cells, and it is also the main medium for transporting secreted products. Centrifuge fresh blood to allow blood cells to settle, and the upper layer of pale yellow clear liquid is plasma. Serum is a gelatinous liquid in plasma that does not contain fibrinogen, and has the function of maintaining normal viscosity, acidity, and osmotic pressure of blood.

It is mainly composed of water and various chemical components, including albumin, alpha 1, alpha 2, beta, gamma globulin, triglycerides, total, alanine aminotransferase, etc. Serum contains various plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc. These substances achieve physiological balance in promoting cell growth or inhibiting growth activity. During the melting process, it is necessary to shake evenly to reduce the production of protein precipitation.

Characteristic: Lack of antibodies against A and B blood group antigens in human AB serum, commonly used to reduce immune response to a very low level. It has been confirmed that human AB serum can increase the growth rate of most human derived cells, and the concentration used is lower than that of mixed serum. More suitable for cell, tissue and organ culture, cell line preservation, and monoclonal antibody development.

Product Description

Valley Biomedical human AB plasma was collected from healthy male donors at FDA approved facilities located within the United States. These individual facility units are collected and converted into serum, which is then collected, filtered, bottled, and cell cultured for mycoplasma, endotoxin, and sterility testing. Test each individual facility unit using FDA approved methods (HBsAg, HIV-1, HIV-2, HCV, HIV-1 Ag or HIV-1 NAT, ALT, and syphilis) to confirm that all necessary viral markers are negative.

The Valley Biomedical Heat Inactivation Protocol involves raising the temperature of the serum to 56 degrees Celsius and maintaining it for 30 minutes. Some precipitates may be obvious, and if necessary, they can be removed by filtration.

*0.1 µ m sterile filtration

*Dry ice transportation. -Frozen storage at 20 to -10 ° C

*Can be thermally inactivated

*Can be irradiated with gamma rays