Bambanker serum-free cell cryopreservation solution

Cryopreservation of mammalian cells is highly valuable and widespread in biological research. In order to prevent cell contamination due to technical reasons (cultivation operation) or physical reasons (incubator failure) that could jeopardize long-term cultivation efforts. In addition, long-term and repeated cultivation and passage can lead to genetic aging and differentiation of cells, which is the primary strategy considered after receiving or generating new cell lines, which is cryopreservation of cells. Cryopreservation is extremely important for maintaining the genetic characteristics of some special cell lines. The basic principle of deep low-temperature preservation of cells is that when the temperature is below -70 ℃, the enzyme activity inside the cell has stopped, that is, metabolism is in a stopped state, so it can be stored for a long time. The key to low-temperature preservation of cells lies in the treatment process from 0 ℃ to -20 ℃. Within this temperature range, ice crystals are needle shaped and highly susceptible to severe cell damage.

How to reduce or avoid ice crystal formation, similar to glycerol used for bacterial cryopreservation, DMSO (dimethyl sulfoxide) is a permeability protectant that can lower the freezing point of cells, reduce ice crystal formation, alleviate free radical damage to cells, and alter the permeability of biofilms to electrolytes, drugs, toxins, and metabolites. The addition of cryoprotectant can lower the freezing point of the solution, and under slow freezing conditions, the water inside the cells gradually seeps out, thereby reducing the formation of ice crystals and avoiding cell damage. This is an important reason why slow freezing is used when freezing cells at low temperatures.

Once transferred from the growth medium to the frozen medium, a slow freezing process must be performed. Although freezing cell lines is a common procedure, problems can arise when there is no suitable freezing equipment, or when additional washing or improper handling is required due to the presence of serum. Of course, this issue was only discovered after cell recovery (rapid melting). Most or all of the recovered cells were found to be dead, and there were even cases of cell differentiation caused by improper freezing processes that were not easily detected. All these issues and concerns can be addressed through Bambaker^TMTo solve the problem of cell freezing culture medium reagent family. Bambanker^TMThe ingredients allow for direct cryopreservation of cells at -80 ° C or liquid nitrogen, thereby avoiding the need for additional equipment and also avoiding time-consuming and complex gradient freezing schemes.

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Product Features

Can freeze cells for a long time at temperatures of -80 ° C or -196 ° C

• High viability after cell recovery

Ready to use freezing medium - no need for program or sequential cooling freezing

• Serum free - no contamination risk

•Can be used for all known cell lines

• Long shelf life

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Save time, effort, and cells（Save time while saving your cells）

Cell cryoculture medium Bambanker^TMAllowing cell cryopreservation at -80 ° C (or liquid nitrogen) avoids the need for additional equipment and avoids time-consuming and complex controlled freezing schemes. Simply (1) centrifuge to harvest cells, (2) aspirate the culture medium, and (3) resuspend the cells in Bambanker^TMMix well in (4) and transfer to a freezing tube (5) for storage at -80 ° C. No need for program cooling or sequential cooling! Bambanker^TMIt is a serum-free cryopreservation medium that can be used immediately and can be stored in the refrigerator for up to two years. Provide 20 milliliter packaging for Bambaker^TMFrozen culture medium is very suitable for independent use by various members of the laboratory. Nowadays, this innovative cell freezing culture medium Bambanker^TMIt has become a market in Japan, characterized by the publication of many articles involving highly sensitive cell lines.

Serum adds variation to long-term storage

All Bambankers^TMThe products do not contain serum. Cryopreservation media containing serum have the disadvantages of fluctuating recovery rates and uncertain composition. The experimental reproducibility of cells frozen in serum containing culture medium may be affected by serum differences between batches, as the composition and concentration of proteins and other biomolecules vary with each batch of serum. Thawing and using cells in this serum containing culture medium may cause problems. Because of Bambaker^TMEach component of is precisely defined, so you can rest assured that cells stored at different times will have consistent efficiency.

Bambanker^TMCan prevent unexpected differentiation (Bambaker)^TMprevents undesired differentiation）

Unexpected cell differentiation may also be a problem for long-term preservation of frozen cells. All Bambanker reagents are designed to reduce this issue. For particularly troublesome cells, Bambanker's DMSO free formula is specially formulated to prevent potential issues related to DMSO containing cryopreservation reagents. Therefore, please join the growing selection of laboratories and use Bambanker to protect their cell lines!

Cell viability and ALP staining of pluripotent stem cells

Upper row: A large number of cells were detected after thawing for two days. After thawing, there is no morphological change in the cells

Bottom row: Bambaker^TMIt will not cause cell differentiation, as all frozen stem cells still produce high levels of alkaline phosphatase, which is the reporter gene for pluripotent stem cells

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Family of Bamboo Cell Cryoprotectants^TM）