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Shanghai Guyan Biotechnology Co., Ltd
Tumor suppressor gene LPP2 antibody
NegotiableUpdate on 05/06
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Overview
Tumor suppressor gene LPP2 antibody related products: tumor susceptibility candidate gene 5 antibody coenzyme antibody $r $n centromyosin gamma 1/enhanced PI3K protein antibody coenzyme YP3A antibody $r $n cell division cycle related protein JPO2 antibody coenzyme YP2D1 antibody $r $n cysteine protease-2 antibody coenzyme PA6 antibody $r $n cardiac cadherin antibody coenzyme P4501B1 antibody
Product Details
Tumor suppressor gene LPP2 antibody
English name |
VANGL1 |
goods number |
GOY-01K2009 |
specification |
50ulThe100ulThe200ul |
sex form |
Liquid |
Antibody source |
Rabbit |
thick degree |
1mg/ml |
Clone type |
Polyclonal |
Asia type |
IgG |
Don't Name;Loop tail protein 2 homolog; Loop-tail protein 2 homolog; LPP2; MGC5338; STB2; STBM2; Strabismus 2;
Research field; tumor Neurobiology signal transduction stem cell
Source of antibodies;Rabbit
Clone type;Polyclonal
Cross reactivity;(predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Product application;WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200(Paraffin sections require antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight;60 kDa
Cell localization; cell membrane
sex State;Liquid
thick Degree;1mg/ml
exempt epidemic Original;KLH conjugated synthetic peptide derived from human VANGL1/LPP2: 151-250/524
Asia Type;IgG
Purification method;affinity purified by Protein A
Preservation conditions;Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
matters needing attention;This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
1. Prepare antigens.
2. Select experimental animals.
3. Animal immunity.
4. Take blood samples for testing to see if immunization is successful.
5. If successfully immunized, kill the experimental animals and collect all serum.
6. Purify the antibody.
7. Identify antibodies. Including purity and specificity.
1） Antigen preparation
Ig is an immunoglobulin that is a good antigenic substance for heterologous animals. Can stimulate heterologous animals to produce anti Ig serum,
After appropriate extraction, anti Ig antibodies, also known as secondary antibodies or anti antibodies, are produced. In most indirect labeling reactions, preparation is required
Anti Ig antibody.
（2） Materials and reagents
1. Extracted animal Ig
2. Freund's adjuvant and Freund's non adjuvant
3. Experimental animal rabbit
4. Other materials and reagents
The Vang family of proteins are integral membrane proteins that are homologues of the Drosophila tissue polarity gene strabismus. The gene encoding for Van Gogh-like protein 1 (Vangl1), also designated Strabismus 2 (STB2),
SduI (Bsp1286I)Specifications500 units
SmaISpecifications1200 units
SmaISpecifications5x1200 units
SmaI, HCSpecifications6000 units
SmaISpecifications2000 units
TaqISpecifications3000 units
TaqISpecifications5x3000 units
XbaISpecifications1500 units
XbaISpecifications5x1500 units
XbaI, HCSpecifications7500 units
XbaISpecifications3000 units
XhoISpecifications2000 units
XhoISpecifications5x2000 units
XhoI, HCSpecifications10000 units
XhoISpecifications5000 units
Bst1107I (BstZ17I)Specifications500 units
Van91I (PflMI)Specifications400 units
Van91I (PflMI)Specifications2000 units
BclISpecifications1000 units
BclISpecifications5000 units
BclISpecifications3000 units
Mph1103I (NsiI)Specifications1000 units
Mph1103I (NsiI)Specifications5000 units
CpoI (RsrII)Specifications200 units
CpoI (RsrII)Specifications1000 units
MunI (MfeI)Specifications300 units
MunI (MfeI)Specifications1500 units
Psp5II (PpuMI)Specifications500 units
SspISpecifications500 units
1. Specific immunogens
The type of immunogen used to produce antibodies significantly affects the specificity of antibodies to target proteins. Choosing antibodies produced against unsuitable or low-quality immunogens will result in unreliable and potentially irreproducible outcomes. Attention should be paid to selecting antibodies that target fully characterized immunogens.
2. Peptides
Synthetic peptide immunogens rarely fold into natural protein like tertiary structures. In addition, they do not exhibit any post-translational modifications unless added to the peptide during the synthesis process. Therefore, antibodies produced using peptides can be used as useful reagents for Western blotting, as target proteins have linearized epitopes due to denaturation and reduction during sample processing. When antibodies need to distinguish between two proteins with highly conserved amino acid sequences, peptides can also be used as immunogens. In these cases, the production of peptides to differential regions increases the likelihood of producing differential antibodies.
3. Natural protein
If purified proteins are used as immunogens, antibodies produced against them may not recognize the linear epitopes of denatured proteins in Western blotting. Some antibodies produced using natural proteins can only recognize natural oxidative conformational epitopes on the protein surface. This is especially true for monoclonal antibodies.