Trypsin solution

Trypin is a serine hydrolase that cleaves the carboxyl side segments of lysine and arginine residues in polypeptide chains, hydrolyzing proteins between cells, disrupting intercellular connections, and causing tissue or adherent cells to disperse into individual cells. The activity of pancreatic enzymes in dispersing cells is related to the characteristics of tissues or cells, the concentration, temperature, and duration of action of pancreatic enzymes. At pH 8.0 and 37 ℃, the ability of pancreatic enzymes to act is *. Therefore, when using pancreatic enzymes, the concentration, temperature, and duration should be carefully controlled to avoid excessive digestion and cell damage. Trypsin solution

Trypsin（Trypin）It is a serine hydrolase that can cleave the carboxyl side segments of lysine and arginine residues in polypeptide chains, hydrolyze proteins between cells, break intercellular connections, and disperse tissues or adherent cells into individual cells. The activity of trypsin dispersing cells is related to the characteristics of tissues or cells, trypsin concentration, temperature, and duration of actionPH 8.0and37At ℃, the ability of pancreatic enzymes to act *, so when using pancreatic enzymes, the concentration, temperature, and time should be carefully controlled to avoid excessive digestion and cell damage.

The working concentration of commonly used pancreatic enzymes is0.25%However, semi adherent cells or cells sensitive to pancreatic enzymes often use low concentrations（0.05%）Pancreatic enzymes are used for cellular digestion. Due toEDTACapable of chelationCa²⁺andMg²⁺To disrupt cell connections and promote cell dissociation, a certain amount of trypsin solution is often addedEDTAMixed use to enhance dissociation effect.

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Precautions

1Due to the varying reactions of different tissues or cells to pancreatic enzymes, operators should determine the optimal digestion time based on the actual situation; The digestion time of cells should not be too long, otherwise it will affect the cell adhesion and growth status;

2When using this product, attention should be paid to aseptic operation to avoid contamination;

3Not suitable for prolonged storage at room temperature or4Long term storage at ℃;

4The2℃～8Thaw at ℃, shake well before use, avoid repeated freezing and thawing, and it is recommended to pack and store in small quantities.

5Due toD-Hank'sBalanced salt solutionNaHCO₃Low content（0.35g/L）Therefore, this typeTrypsin solutionNot for use5% CO₂The environment, if placedCO₂The culture phase and solution will quickly become acidic, so attention should be paid when using it;

6This product is only used for scientific research or further research purposes and is not intended for diagnosis and treatment.