HEK293 serum-free basic culture medium

HEK293 serum-free basic culture medium is a cell culture medium independently developed and produced by the Ponosi R&D team without animal derived component free (ADCF). It can support rapid cell adaptation in various 293 cell lines (293, 293T, 293A, etc.), with the characteristics of high cell viability, long maintenance time, high transfection efficiency, and high product yield.

Applicable cell lines

HEK293 serum-free basic culture mediumIt is a cell culture medium without Animal Derived Component Free (ADCF) independently developed and produced by the Ponosi R&D team. It can support rapid cell adaptation in various 293 cell lines (293, 293T, 293A, etc.), with the characteristics of high cell viability, long maintenance time, high transfection efficiency, and high product yield.

HEK293 serum-free basic culture mediumcharacteristic

1. This product does not contain any animal derived component free (ADCF).

2. This product contains amino acids, vitamins, glucose, lipids, inorganic salts, growth factors, hydrolysates, and trace elements, but does not containPolypeptides.

culture conditions

1. The cultivation temperature is 37 ℃. In batch and batch fed culture processes, the recommended initial cell seeding density is0.5-1×106cells/mL。

2. Under non automatic pH control conditions, it is recommended to CO2The concentration is controlled at 5%.

3. Process conditions such as pH, DO, and temperature can be set according to the results of process development or platform process parameters.

Medium adaptation (recommended)

1. Direct adaptation: In the initial cultivation stage, cells are cultured at an initial density of 0.5-1 × 106Inoculate cells/mL and directly replace the culture medium withHEK 293 serum-free basic medium was used for cell culture. After 2-3 generations of cell culture and stable cell growth, subsequent experiments can be conducted.

2. Indirect adaptation:HEK293 serum-free basic culture mediumFollow the sequence of (75:25, 50:50, 25:75, 0:100) with the original cell culture mediumProportional mixing is used for gradual passage, and it is recommended to pass 2-3 passages of each mixture, which can be adjusted according to the growth status of the cells; When the culture medium is completely replacedHEK293After serum-free basic culture medium, continue to passage 3-5 times until cell growth stabilizes before conducting subsequent experiments.

(Note: The specific cultivation process can be adjusted according to the actual project requirements)

data support

Cell cryopreservation

Use the frozen culture medium made from this product for cell cryopreservation, it is recommended to add 10% DMSO, and the cell cryopreservation density should be 1.0-2.0 × 107cells/mL。

Storage conditions

2-8 ℃, stored away from light, with a shelf life of 12 months.