Black gum worm removal culture medium

Black gum worms "and their decomposition complexes are common cellular pollutants that coexist with cells and are passaged with them. Antibiotics are usually ineffective against them. Black gum worms compete with cells for growth, which is detrimental to cell growth and can lead to cell death in severe cases. At present, many cells are contaminated by "black glue worms", which has a great impact on cell culture and subsequent experiments.

The common characteristics of cells contaminated with "black glue worms" are as follows:（1）The culture medium is not turbid, but when observing cells under a microscope, there are many "small black spots" around the cells and in the culture medium, which gradually increase with the extension of fine culture time. Changing the culture medium or washing the cells cannot remove them; （2）Cells contaminated with "small black spots" consume nutrients quickly and require frequent replacement of culture media; （3）The cells contaminated with "black glue worms" grow slowly, have poor cell state, severe vacuolization, and may even lead to changes in cell morphology.

Black gum worm removal culture mediumOur company isBiomocinThe new generation of products developed on the basis of the product contains special ingredients for removing "black gum worms". This product has been tested on over a hundred types of cells and verified through long-term experiments. It is harmless to cells and has a significant effect on removing "black glue insect" pollutionIt can effectively kill and inhibit the "black glue bug".

Product Information

Product usage instructions

1According to the characteristics of the cultured cells, extract the black fungusPreparation of clearing culture mediumCreate the corresponding culture medium.

2, will50～100Spread thousands of cells60 mmIn the cell culture dish, add4 mlThe cells can be cultured in the same medium as the normal cell culture method, and the cells can also be incubated in a culture vessel directly after the removal of the "black gum worm".

3The next day, replace the culture medium with fresh "black glue worms" and replace the culture vessels with new ones; Afterwards, every2～3Day replacement1Wash the cells with sterile balanced salt solution when changing the secondary culture medium1～2Secondly; If the cell density is too high during the process, please maintain an appropriate cell density (adherent cells may need to be digested with trypsin).

4Use "black glue worms" to remove the culture medium3Clear effect will be visible after the day, continuous use12～14The "black glue bug" can be removed in a timely manner. If the pollution caused by the "black glue bug" is severe, the treatment can be extended3～5Oh my god.

5As there may still be sources of pollution in the environment, to avoid further contamination of cells by "black glue worms", it is recommended that you continue to use the "black glue worm" removal culture medium after the "black glue worm" has been removed to achieve the preventive effect.

Precautions

1、This product has been0.22μmFilter and sterilize, when using this product, attention should be paid to aseptic operation to avoid contamination;

2To maintain the optimal performance of this product,It is not advisable to place it in a room temperature or high temperature environment for a long time;

3This product is for research or further production use only and is not intended for diagnosis or treatment.