This reagent kit is specifically developed for the identification of the authenticity and adulteration of ingredients in traditional Chinese medicine pills.
Traditional Chinese medicine pills are spherical or quasi spherical solid preparations made of fine powder or extract of medicinal materials plus appropriate adhesive or auxiliary materials, and are one of the oldest dosage forms of traditional Chinese patent medicines and simple preparations.PCRofDNAThe testing scheme is becoming the gold standard for the authenticity identification of traditional Chinese medicine, but the processed Chinese medicine pills have complex ingredients, including adhesives and excipients, which can affect their authenticityPCRThe reaction has an inhibitory effect, leading toDNAThe detection method cannot be effectively applied in this field. This test kit is developed independentlyDNAExtraction methods, for somePCRInhibiting ingredients for effective cleaning, thereby achieving effectiveDNAExtract purity. extractedDNAdirectly applicablePCRThe reaction provides a reliable detection scheme for the authenticity identification and adulteration detection of medicinal materials.
This reagent kit adopts the extraction scheme of magnetic bead method, and after multiple washings, the inhibition is removedPCRRelated components of the reaction, extractedDNAdirectly applicablePCROr fluorescence quantificationPCRTesting technology. For deeply processed foodsThis experiment can also be usedBox extractionDNAUsed for the detection of animal and plant components, genetically modified organisms, and allergens.