HEK 293T Host Cell Residual DNA Quantification Kit

The HEK 293T cell residual DNA quantification kit is a molecular detection reagent based on fluorescence quantitative PCR (qPCR), used to detect residual DNA in 293T cells used in biological products. This kit is reliable and fast for detection, capable of achieving sensitive (as low as 15 pg DNA/mL sample detection) and specific quantification of human cell DNA containing 293T.

Meanwhile, biopharmaceutical companies can also use online testing methods to confirm the rationality and effectiveness of pure chemical Yida. The fluorescence quantitative PCR method is becoming the gold standard for residual host cell DNA detection, which can detect fg level residual DNA of human cell lines within 40 minutes while maintaining excellent specificity and sensitivity. This kit adopts the Taqman probe method for specific design and a strong anti inhibition PCR reaction system to ensure the authenticity and effectiveness of experimental results.

The host cell DNA residue quantification reagent can detect different levels of host cell DNA residue in biopharmaceutical products. The system provides:

• Adopting tested real-time quantitative PCR technology for high-sensitivity quantitative detection

Using 5 reference quantitative standards at 10 times the standard, create a standard curve with a lower limit of quantification of 300fg

• PCR reaction can be completed in fast mode within 40 minutes

Using UNG to decontaminate PCR premix, reducing the probability of contamination

The detection reagent contains VIC/HEX fluorescent labeled internal amplification control IPC, ruling out PCR inhibited amplification failure

Maintain good consistency of results within the expected range of DNA fragment sizes

Accurate, reliable, and reproducible results

*Note: Due to the high similarity between genomes, there may be cross PCR amplification between primate species such as monkeys (Vero), chimpanzees, and orangutans.