This kit can be used for quantitative analysis of residual DNA of Escherichia coli in biopharmaceuticals.
Escherichia coli residual DNA quantification kitIt is a molecular detection reagent based on fluorescence quantitative PCR (qPCR), used to detect residual Escherichia coli DNA in biological products. This kit is reliable and fast for detection, capable of achieving sensitive (as low as 15 pg DNA/mL sample detection) and specific quantification of Escherichia coli DNA.
Meanwhile, biopharmaceutical companies can also use online testing methods to confirm the rationality and effectiveness of pure chemical Yida. The fluorescence quantitative PCR method is becoming the gold standard for residual E. coli DNA detection, which can detect FG level residual DNA of E. coli within 40 minutes while maintaining excellent specificity and sensitivity. This kit adopts the Taqman probe method for specific design and a strong anti inhibition PCR reaction system to ensure the authenticity and effectiveness of experimental results.
The host cell DNA residue quantification reagent can detect different levels of host cell DNA residue in biopharmaceutical products. The system provides:
• Adopting tested real-time quantitative PCR technology for high-sensitivity quantitative detection
Using 5 reference quantitative standards at 10 times the standard, create a standard curve with a lower limit of quantification of 300fg
• PCR reaction can be completed in fast mode within 40 minutes
Using UNG to decontaminate PCR premix, reducing the probability of contamination
The detection reagent contains VIC/HEX fluorescent labeled internal amplification control IPC, ruling out PCR inhibited amplification failure
Maintain good consistency of results within the expected range of DNA fragment sizes
Accurate, reliable, and reproducible results

